A5020772093- Thyroid Disorders and Treatments
- Congenital heart defects research
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- T-cell and B-cell Immunology
- Renal and related cancers
- Growth Hormone and Insulin-like Growth Factors
- RNA and protein synthesis mechanisms
- Effects and risks of endocrine disrupting chemicals
- RNA Research and Splicing
- RNA modifications and cancer
- Genetic Syndromes and Imprinting
- Thyroid Cancer Diagnosis and Treatment
- FOXO transcription factor regulation
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Birth, Development, and Health
- Genomics and Chromatin Dynamics
- Genetics and Neurodevelopmental Disorders
- Toxic Organic Pollutants Impact
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Molecular Biology Techniques and Applications
- Animal testing and alternatives
- Craniofacial Disorders and Treatments
University of Naples Federico II
2015-2024
Institute for Experimental Endocrinology and Oncology
2019-2024
Federico II University Hospital
2015-2024
National Research Council
2019-2021
Texas A&M University
2020
Biogem
2006-2018
Stazione Zoologica Anton Dohrn
1996-2007
Agrobioinstitute
2007
Ceinge Biotecnologie Avanzate (Italy)
2005-2006
Icahn School of Medicine at Mount Sinai
2002
TTF-1, a homeodomain-containing transcription factor, which is required for the specific expression of thyroglobulin and thyroperoxidase gene promoters in differentiated thyroid cell lines, expressed at very beginning rat differentiation. TTF-1 mRNA detected endodermal cells rudiment embryo precedes two known target genes by 5 days. No delay observed between appearance protein, shows clear nuclear localization. In adult thyroid, present only endoderm-derived follicular cells. Two additional...
The thyroid-stimulating hormone/thyrotropin (TSH) is the most relevant hormone in control of thyroid gland physiology adulthood. TSH effects on are mediated by interaction with a specific receptor (TSHR). We studied role TSHTSHR signaling morphogenesis and differentiation mouse embryo using lines deprived either (pit(dw)pit(dw)) or functional TSHR (tshr(hyt)tshr(hyt) TSHR-knockout lines). results reported here show that absence TSHR, develops to normal size, whereas expression...
Congenital hypothyroidism (CH) is a common endocrine disorder with an incidence of 1:3000-4000 at birth. In 80-85% cases, CH caused by defects in thyroid organogenesis, resulting absent, ectopically located, and/or severely reduced gland [thyroid dysgenesis (TD)]. Mutations genes controlling development have demonstrated that few TD Mendelian trait. However, accumulating evidence supports the view genetics are complex, possibly polygenic/multifactorial basis. A higher prevalence congenital...
Background Although the mouse is animal model most widely used to study pathogenesis and treatment of human diseases, reference values for biochemical parameters are scanty or lacking frequently strains. We therefore evaluated these in C57BL/6J, 129SV/EV C3H/HeJ mice. Methodology/Principal Findings measured by dry chemistry 26 analytes relative electrolyte balance, lipoprotein metabolism, muscle/heart, liver, kidney pancreas functions, automated blood counter 5 hematological 30 animals (15...
Congenital hypothyroidism with thyroid dysgenesis (TD) is a frequent human condition characterized by elevated levels of TSH in response to reduced hormone levels. genetically heterogeneous disease. In the majority cases studied, no causative mutations have been identified and very often disease does not show Mendelian transmission. However, approximately 5% cases, it can be consequence genes encoding receptor or transcription factors TITF1, FOXE1, PAX8. We report here that mouse models,...
The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing protein implicated in the activation of thyroid-specific gene expression. Here we report that TTF-1 capable activating from thyroglobulin and, to lesser extent, thyroperoxidase promoters nonthyroid cells. Full transcriptional promoter by requires presence at least two binding sites. activates via functionally redundant domains as suggested competition experiments, could use common intermediary factor....
Transformation of the thyroid cell line FRTL-5 results in loss or reduction differentiation as measured by expression thyroglobulin and thyroperoxidase, two proteins whose genes are exclusively expressed follicular cells. The biochemical mechanisms leading to this phenomenon were investigated three lines obtained transformation cells with Ki-ras, Ha-ras, polyomavirus middle-T oncogenes. With ras oncogenes, leads undetectable thyroperoxidase genes. However, responsible for extinction...
Abstract Titf2/foxe1 is a forkhead domain‐containing gene expressed in the foregut, thyroid, and cranial ectoderm of developing mouse. Titf2 null mice exhibit cleft palate either sublingual or completely absent thyroid gland. In humans, mutations encoding for transcription factor‐2 (TTF‐2) result Bamforth syndrome, characterized by agenesis, palate, spiky hair, choanal atresia. Here, we report detailed expression pattern TTF‐2 protein during mouse embryogenesis show its presence structures...
In the thyroid, H 2 O is produced at apical pole of thyrocytes by one or two NADPH oxidases (NOX), Duox1/2 proteins. The onset Duox expression was analysed immunohistochemistry in developing mouse thyroid parallel with thyroglobulin (Tg) iodination and other differentiation markers. proteins were found embryonic day (E) 15.5 mainly localised thyrocytes. Tg detected 1 before (E14.5) concomitant both Na + /I − symporter (NIS; E15.5). role TSH regulating accumulation evaluated thyroids adult...
Abstract Epidemiologic and experimental studies have associated changes of blood glucose homeostasis to Bisphenol A (BPA) exposure. We took a toxicogenomic approach investigate the mechanisms low-dose (1 × 10 −9 M) BPA toxicity in ex vivo cultures primary murine pancreatic islets hepatocytes. Twenty-nine inhibited genes were identified none exposed Although their expression was slightly altered, impaired cellular level, as whole, resulted specific phenotypic changes. Damage mitochondrial...
Gastric cancer (GC) is a leading cause of cancer-related deaths in the world. Molecular heterogeneity major determinant for clinical outcomes and an exhaustive tumor classification currently missing. Histologically normal tissue adjacent to (NAT) commonly used as control studies, nevertheless recently published paper described unique characteristics NAT several types. Little known about global gene expression profile gastric (gNAT) which could be effective tool more realistic definition GC...
The phosphorylation of thyroid transcription factor-1 (TTF-1), a homeodomain-containing factor that is required for thyroid-specific expression the thyroglobulin and thyroperoxidase gene promoters, has been studied. Phosphorylation occurs on maximum seven serine residues are distributed in three tryptic peptides. Mutant derivatives TTF-1, with alanine replacing serines sites, have constructed used to assess functional relevance TTF-1 phosphorylation. DNA binding activity appears be...
Transformation of the thyroid cell line FRTL-5 results in loss or reduction differentiation as measured by expression thyroglobulin and thyroperoxidase, two proteins whose genes are exclusively expressed follicular cells. The biochemical mechanisms leading to this phenomenon were investigated three lines obtained transformation cells with Ki-ras, Ha-ras, polyomavirus middle-T oncogenes. With ras oncogenes, leads undetectable thyroperoxidase genes. However, responsible for extinction...
In Italy, the surveillance of congenital hypothyroidism (CH) is performed by Italian National Registry Infants with CH (INRICH). Up to now, about 3600 infants are recorded in INRICH, and a high number twins included.Our objective was estimate risk multiple single deliveries compare neonatal features from general population.The population INRICH 1989-2000 investigated.A more than 3-fold higher frequency found population, for first time, it possible incidence (10.1 10,000) (3.2 10,000 live...
The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of embryonic foregut. When how organ specific programs regulate development into structures distinct shapes, positions functions is incompletely understood. To characterize, at least in part, genetic basis these events, we have employed laser capture microdissection microarray analysis to define gene expression mouse lung primordia E10.5. By comparing transcriptome each that whole embryo well other, broadly...