A5053724465- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Hepatitis B Virus Studies
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Phytochemicals and Antioxidant Activities
- RNA and protein synthesis mechanisms
- Connexins and lens biology
- Virus-based gene therapy research
- Melanoma and MAPK Pathways
- Liver Disease Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Natural product bioactivities and synthesis
- Cytokine Signaling Pathways and Interactions
- Biochemical effects in animals
- Epigenetics and DNA Methylation
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Computational Drug Discovery Methods
- Antimicrobial Peptides and Activities
- Cancer-related gene regulation
- Metabolism, Diabetes, and Cancer
University of Campania "Luigi Vanvitelli"
2016-2025
Creative Research Enterprises (United States)
2018
University of Catania
2018
University of Naples Federico II
1998-2014
Stazione Zoologica Anton Dohrn
2005
MicroRNAs are small non-coding RNAs that modulate gene expression at post-transcriptional level, playing a crucial role in cell differentiation and development. Recently, some reports have shown limited number of mammalian microRNAs also involved anti-viral defense. In this study, the analysis hepatitis B virus (HBV) genome by computer program MiRanda led to identification seven sites potential targets for human liver microRNAs. These were found be clustered 995-bp segment within viral...
Hepatocellular carcinoma (HCC) is the third cause of cancer-related deaths worldwide. Sorafenib only approved drug for patients with advanced HCC but has shown limited activity. microRNAs (miRs) have been involved in several neoplasms including suggesting their use or targeting as good tools treatment. The purpose this study was to identify novel approaches sensitize cells sorafenib through miRs. miR-423-5p validated positive regulator autophagy cell lines by transient transfection miR and...
To study in HBsAg chronic carriers the expression of liver hsa-miR-125a-5p and its correlation with HBV-DNA values clinical presentation, 27 consecutive Caucasian, HBsAg/anti-HBe/HBV-DNA-positive patients who were naive to nucleos(t)ide analogues interferon therapy had no marker HCV, HDV or HIV infection history alcohol intake enrolled. For each patient, HBV DNA quantified by real-time PCR relation β-globin RNU6B, respectively. Liver fibrosis necroinflammation graded applying Ishak's scoring...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel RNA virus affecting humans, causing form of pulmonary disorder named COVID-19, declared pandemic by the World Health Organization. MicroRNAs (miRNA) play an emerging and important role in interplay between viruses host cells. Although impact miRNAs on SARS-CoV-2 infection has been predicted, experimental data are still missing. This study started bioinformatics prediction cellular potentially targeting viral RNAs; then,...
Abstract Osteosarcoma (OS) is the most prevalent and fatal type of bone tumor. It characterized by great heterogeneity genomic aberrations, mutated genes, cell types contribution, making therapy patients management particularly challenging. A unifying picture molecular mechanisms underlying disease could help to transform those challenges into opportunities. This review deeply explores occurrence in OS large-scale RNA regulatory networks, denominated “competing endogenous network” (ceRNET),...
Sorafenib is an antitumor drug for treatment of advanced hepatocellular carcinoma (HCC). It acts as a multikinase inhibitor suppressing cell proliferation and angiogenesis. Human microRNA‐125a‐5p (miR‐125a) endowed with similar activities frequently downregulated in HCC. Looking potential microRNA‐based mechanism action the drug, we found that sorafenib increases cellular expression miR‐125a cultured HuH‐7 HepG2 HCC cells. Upregulation microRNA inhibited by suppression sirtuin‐7,...
The discovery of bioactive compounds from natural sources entails an extremely lengthy process due to the timescale and complexity traditional methodologies. In our study, we used a rapid NMR based metabolomic approach as tool identify secondary metabolites with anti-proliferative activity against panel human colorectal cancer cell lines different mutation profiles. For this purpose, fourteen Fabaceae species Mediterranean vegetation were investigated using double screening method: 1H...
Hepatocellular carcinoma (HCC) is among the most aggressive and lethal human tumors. Many functional studies have demonstrated role of non-coding RNAs (ncRNA), particularly microRNAs (miRNA), in regulation hepatocarcinogenesis driving pathways. MiR-125a-5p (miR-125a) has been consistently reported as an oncosuppressive miRNA, vivo vitro. However, its HCC relevant targets molecular mechanisms are still largely unknown. Here, a genome-wide perspective whole miR-125a targetome achieved. In...
ABSTRACT Male fertility declines during aging. This process mainly affects spermatogonia and Sertoli cells, leading to impaired spermatogenesis poor‐quality sperm production. Circular RNAs (circRNAs) are covalently closed RNA molecules produced by backsplicing. In the field of male reproduction, circRNAs boast great potential in regulation morpho‐functional skills. However, their role age‐related reproductive anomalies remains largely elusive. Here, we analyzed phenotype aged mouse...
Oxidative stress has been proposed to be an important factor in the pathogenesis of Alzheimer's disease (AD), playing a central role amyloid β-protein (Aβ) generation and neuronal apoptosis. damage directly correlates with presence Aβ deposits. oxidative jointly induce death, deposits, gliosis, memory impairment AD. In order counteract AD neurodegeneration, inhibition vicious cycle oxidation is attractive therapeutic strategy, antiamyloidogenic antioxidant herbal drugs could represent...
Human microRNA-125a-5p (miR-125a) is expressed in most tissues where it downregulates the expression of membrane receptors or intracellular transductors mitogenic signals, thus limiting cell proliferation. Expression this miRNA generally increases with differentiation whereas downregulated several types tumors, such as breast, lung, ovarian, gastric, colon, and cervical cancers, neuroblastoma, medulloblastoma, glioblastoma, retinoblastoma. In study, we focused on hepatocellular carcinoma...
This study investigated the protective effects of intravitreal Resolvin D1 (RvD1) against LPS-induced rat endotoxic uveitis (EIU). RvD1 was administered into right eye at a single injection 5 μL volume containing 10-100-1000 ng/kg 1 h post-LPS (200 μg, Salmonella minnesota) thefootpad Sprague-Dawley rats. 24 later, enucleated and examined for clinical, biochemical, immunohistochemical evaluations. significantly dose-dependently decreased clinical score attributed to EIU, starting from dose...
This study aims to explore the complex role of cannabinoid type 1 receptor (CB1) signaling in gastrocnemius muscle, assessing physiological processes both CB1+/+ and CB1−/− mice. The primary focus is enhance our understanding how CB1 contributes mitochondrial homeostasis. At tissue level, mice exhibit a substantial miRNA-related alteration muscle fiber composition, characterized by an enrichment oxidative fibers. absence induces significant increase capacity supported elevated in-gel...
The discovery of siRNAs as the mediators RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into optimize potency, stability and pharmacokinetic properties vivo. Here, we synthesize test effects RNA-3'-PNA chimeras on siRNA functioning stability. We demonstrate that chemical compatible with machinery, because all PNA-modified can efficiently mediate specific gene silencing mammalian cells. Furthermore, find modification...