A5091053991- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Advanced Sensor and Control Systems
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Genomics, phytochemicals, and oxidative stress
- Hematopoietic Stem Cell Transplantation
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- Extracellular vesicles in disease
- Gene Regulatory Network Analysis
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- Bioinformatics and Genomic Networks
- Advanced Measurement and Detection Methods
- Seismic Performance and Analysis
- Non-Destructive Testing Techniques
- interferon and immune responses
- Anesthesia and Neurotoxicity Research
- Structural Response to Dynamic Loads
- Neuroscience and Neuropharmacology Research
- Structural Behavior of Reinforced Concrete
The Eighth Hospital of Xi'an
2025
Beijing University of Agriculture
2024
Stanford University
2011-2024
Collaborative Innovation Center of Chemistry for Energy Materials
2022-2024
University of Science and Technology of China
2022-2024
Qingdao Municipal Hospital
2020-2024
Qingdao University
2020-2024
Shijiazhuang Tiedao University
2021-2024
Ministry of Agriculture and Rural Affairs
2024
Second Affiliated Hospital of Nanchang University
2023
Recent studies suggest a link between mitochondria and proinflammatory cytokine generation. We previously demonstrated that overexpression of mitochondrial chaperone glucose-regulated protein75 (Grp75/mortalin) protects mitochondria. In this study we investigated the modulation lipopolisaccharide (LPS)-induced inflammatory response microglial BV-2 cells by Grp75. demonstrate LPS-induced activation promotes significant metabolic changes suppressing function increasing glycolysis....
The Hippo pathway controls metazoan organ growth by regulating cell proliferation and apoptosis. Many components have been identified, but our knowledge of the composition structure this is still incomplete. Using existing as baits, we generated mass spectrometry a high-confidence Drosophila protein-protein interaction network (Hippo-PPIN) consisting 153 proteins 204 interactions. Depletion 67% RNA interference regulated transcriptional coactivator Yorkie (Yki) either positively or...
Following transient forebrain ischemia, astrocytes play a key role in determining whether or not neurons the hippocampal CA1 sector go on to die delayed fashion. MicroRNAs (miRNAs) are novel class of RNAs that control gene expression at post‐transcriptional level and miR‐29 family is highly expressed astrocytes. In this study we assessed levels hippocampus following ischemia found after short periods reperfusion, miR‐29a significantly increased resistant dentate gyrus, but decreased...
Reactive gliosis is a hallmark of brain pathology and the injury response, yet extent to which astrocytes proliferate, whether this central astrogliosis still controversial. We determined fraction mature that proliferate in mouse stroke model using unbiased stereology as function distance from infarct edge. Cumulatively 11.1±1.2% Aldh1l1+ within 400 µm cortical penumbra incorporate BrdU first week following stroke, while overall number does not change. The proliferating fell sharply with...
Interactome mapping and functional genomics in Drosophila reveal common specific components of a mitogen-activated protein kinase pathway.
Insulin regulates an essential conserved signaling pathway affecting growth, proliferation, and metabolism. To expand our understanding of the insulin pathway, we combine biochemical, genetic, computational approaches to build a comprehensive Drosophila InR/PI3K/Akt network. First, map dynamic protein-protein interaction network surrounding core using bait-prey interactions connecting 566 proteins. Combining RNAi screening phospho-specific antibodies, find that 47% interacting proteins...
Brain ischemia causes immediate and delayed cell death that is exacerbated by inflammation. Recent studies show hypocretin-1/orexin-A (Hcrt-1) reduces ischemic brain injury, Hcrt-positive neurons modulate infection-induced Here, we tested the hypothesis Hcrt plays a protective role against modulating inflammation.Orexin/ataxin-3 (AT) mice, transgenic strain in which Hcrt-producing degenerate early adulthood, wild-type mice were subjected to transient middle cerebral artery occlusion (MCAO)....
MicroRNA (miR)-200c increases rapidly in the brain after transient cerebral ischemia but its role poststroke injury is unclear. Reelin, a regulator of neuronal migration and synaptogenesis, predicted target miR-200c. We hypothesized that miR-200c contributes to from by targeting reelin.Brain infarct volume, neurological score levels miR-200c, reelin mRNA, protein were assessed mice subjected 1 hour middle artery occlusion with or without intracerebroventricular infusion antagomir, mimic,...
In the present study we assessed efficacy of exosomes harvested from human and mouse stem cell cultures in protection primary astrocyte neuronal following vitro ischemia, against ischemic stroke vivo. Cell media was collected neural (NSC) or induced pluripotent cell-derived cardiomyocyte (iCM) cultures. Exosomes were extracted purified by polyethylene glycol complexing centrifugation, exosome size concentration determined with a NanoSiteTM particle analyzer. applied to cortical prior to,...
The transcriptional complexity of mammalian cells suggests that they have broad abilities to respond specific environmental stimuli and physiologic contexts. These were not apparent a priori from the structure genomes, but been identified through detailed transcriptome analyses. In this study, we examined transcriptomes innate immune system, human neutrophils, using RNA sequencing (RNAseq).We sequenced poly-A nine individual samples corresponding phenotypes: three children with active,...
Metabolic syndrome is a chronic metabolic disorder that has turned into severe health problem worldwide. A previous study reported large yellow tea exhibited better anti-diabetic and lipid-lowering effects than green tea. Nevertheless, the potential mechanisms are not yet understood. In this study, we examined prevention of water extract (LWE) on using leptin receptor knockout (Lepr−/−) rats. Seven-week-old male Lepr−/− wild type (WT) littermate rats were divided control group (KO) (n = 5),...
Escape from metastable states in self-assembly of colloids is an intractable problem. Unlike the commonly adopted approach thermal annealing, recently developed enthalpy-mediated strategy provided a different option to address this dilemma dynamically controllable manner at room temperature. However, it required complex catalytic-assembly DNA strand-displacement circuitry mediate interaction between multiple components. In work, we present simple but effective way achieve DNA-functionalized...
One of the pharmacological effects celastrol (Cel) is amelioration acute liver injury. In this study, we explored mechanism Cel underlying alleviation injury induced by traumatic hemorrhagic shock (THS). The THS model was developed from Sprague-Dawley rats through transverse fractures, blood loss and fluid infusion. Then, were intraperitoneally injected with 0.5, 1, 1.5 mg/kg Cel. in tail vein lentivirus-mediated small interfering RNA (siRNA) negative control (siNC), siRNA targeting heat...
Microcystin-LR (MC-LR) is a potent heptapeptide hepatotoxin at high doses, but its underlying mechanism of promoting liver cell proliferation low doses unclear. The transcription factor nuclear erythroid 2-related 2 (Nrf2) key in mediating the protective antioxidant response against various environmental toxicants, emerging data suggest that constitutive activation Nrf2 contributes to malignant phenotype. purpose this study was characterize interactions and effects on induced by MC-LR...
X-linked congenital generalized hypertrichosis (Online Mendelian Inheritance in Man 307150) is an extremely rare condition of hair overgrowth on different body sites. We previously reported linkage a large Mexican family with cosegregating deafness and dental palate anomalies to Xq24-27. Using SNP oligonucleotide microarray analysis whole-genome sequencing, we identified 389-kb interchromosomal insertion at extragenic palindrome site Xq27.1 that completely cosegregates the disease. Among...
Elevated extracellular concentrations of the excitatory transmitter glutamate are an important cause neuronal death in a variety disorders nervous system. The and rates clearance production were measured medium primary cultures from mouse neocortex containing neurons, astrocytes, or both cell types. Measurements performed presence absence 2 mM glutamine with without injury caused by 5-h exposure to hypoxia 500 μM N-methyl-D-aspartate freeze-thaw cycle. High generation (0.5-0.8 μM/min 0.4-ml...