A5065469279- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Postharvest Quality and Shelf Life Management
- Light effects on plants
- Genetics and Plant Breeding
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- Plant responses to elevated CO2
- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Crop Yield and Soil Fertility
- Plant Micronutrient Interactions and Effects
- Plant Physiology and Cultivation Studies
- Plant pathogens and resistance mechanisms
- Weed Control and Herbicide Applications
Universitat Politècnica de València
2014-2024
Consejo Superior de Investigaciones Científicas
2014-2024
Instituto de Biología Molecular y Celular de Plantas
2015-2024
Universidad Autónoma de Baja California Sur
2002-2019
Planta
2016
University of Bonn
2016
Polytechnic University of Puerto Rico
2008
Centro Nacional de Biotecnología
1997-2006
Universidad Autónoma de Madrid
1997-2006
University of Fribourg
2004
Hydrogen peroxide induced the accumulation of free benzoic acid (BA) and salicylic (SA) in tobacco (Nicotiana tabacum L. cv Xanthi-nc) leaves. Six hours after infiltration with 300 mM H2O2, levels BA SA leaves increased 5-fold over detected control The was preceded by rapid activation 2-hydroxylase (BA2H) H2O2-infiltrated tissues. This enzyme catalyzes formation from BA. Enzyme could be reproduced vitro addition H2O2 or cumene hydroperoxide to assay mixture. most effective when applied at 6...
Summary Flowering relies on signaling networks that integrate endogenous and external cues. Normally, plants flower at a particular season, reflecting day length and/or temperature However, can surpass this seasonal regulation show precocious flowering under stress environmental conditions. Here, we UV‐C light activates the transition to in Arabidopsis thaliana through salicylic acid (SA). Moreover, SA also regulates time non‐stressed plants, as SA‐deficient are late flowering. The of by...
Nitric oxide (NO) is an important signaling compound in prokaryotes and eukaryotes. In plants, NO regulates critical developmental transitions stress responses. Here, we identify a mechanism for sensing that coordinates responses throughout development based on targeted degradation of plant-specific transcriptional regulators, the group VII ethylene response factors (ERFs). We show N-end rule pathway proteolysis targets these proteins destruction presence NO, establish them as regulators...
Exposure of Arabidopsis thaliana to ozone results in the expression a number defense-related genes that are also induced during hypersensitive response. A potential common link between activation defense gene response and by treatment is production active oxygen species accumulation hydrogen peroxide. Here we report salicylic acid accumulation, which can be peroxide required for both systemic acquired resistance, induction some, but not all, ozone-induced mRNAs examined. In addition, show...
Salicylic acid (SA) is a likely endogenous regulator of localized and systemic disease resistance in plants. During the hypersensitive response Nicotiana tabacum L. cv Xanthi-nc to tobacco mosaic virus (TMV), SA levels rise dramatically. We studied biosynthesis healthy TMV-inoculated by monitoring its precursors extracts leaves cell suspensions. In leaves, stimulation accumulation accompanied corresponding increase benzoic acid. 14C-Tracer studies with suspensions mock-or indicate that label...
Pathways of salicylic acid (SA) biosynthesis and metabolism in tobacco have been recently identified. SA, an endogenous regulator disease resistance, is a product phenylpropanoid formed via decarboxylation trans-cinnamic to benzoic its subsequent 2-hydroxylation SA. In mosaic virus-inoculated leaves, newly synthesized SA rapidly metabolized O-beta-D-glucoside methyl salicylate. Two key enzymes involved metabolism: 2-hydroxylase, which converts UDPglucose:SA glucosyltransferase (EC 2.4.1.35),...
Active oxygen species have been postulated to perform multiple functions in plant defense, but their exact role resistance diseases is not fully understood. We recently demonstrated H2O2-mediated disease transgenic potato (Solanum tuberosum) plants expressing a foreign gene encoding glucose oxidase. In this study we provide further evidence that the effective against broad range of pathogens. investigated mechanisms underlying plants. The constitutively elevated levels H2O2 induced...
Summary One of the mechanisms involved in chromatin remodelling is so‐called ‘histone replacement’. An example such a mechanism substitution canonical H2A histone by variant H2A.Z. The ATP‐dependent complex SWR1 responsible for this action yeast. We have previously proposed existence an SWR1‐like Arabidopsis demonstrating genetic and physical interaction components SEF, ARP6 PIE1, which are homologues yeast Swc6 Arp6 proteins core ATPase Swr1, respectively. Here we show that H2A.Z, but not...
Salicylic acid (SA) is a likely endogenous signal in the development of systemic acquired resistance (SAR) some dicotyledonous plants. In tobacco mosaic virus (TMV)-resistant Xanthi-nc tobacco, SA levels increase systemically following inoculation single leaf with TMV. To determine extent to which increases result from export inoculated leaf, produced TMV-inoculated or healthy leaves was noninvasively labeled 18O2. Spatial and temporal distribution 18O-SA indicated that most detected tissues...
Abstract Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. Despite its reported regulatory functions, it remains unclear how NO is synthesized in plants. We have generated triple nia1nia2noa1-2 mutant that impaired nitrate reductase (NIA/NR)- and Oxide-Associated1 (AtNOA1)-mediated biosynthetic pathways. content roots nia1nia2 noa1-2 plants was lower than wild-type below the detection limit NIA/NR- AtNOA1-mediated biosynthesis were thus...
Abstract Plant response to mechanical injury includes gene activation both at the wound site and systemically in nondamaged tissues. The model developed for wound-induced of proteinase inhibitor II (Pin2) potato (Solanum tuberosum) tomato (Lycopersicon esculentum) establishes involvement plant hormones abscisic acid jasmonic (JA) as key components signal transduction pathway. To assess Arabidopsis thaliana role these regulating expression, we isolated wound- JA-inducible genes by...
Summary Plants react to mechanical damage by activating a set of genes, the products which are thought serve defensive functions. In solanaceous plants, cell wall‐derived oligosaccharides and plant hormones jasmonic acid ethylene participate in signalling network for wound‐induced expression proteinase inhibitors other defence‐related both locally damaged systemic non‐damaged leaves. Here we show that Arabidopsis thaliana , these components interact novel ways activate distinct responses....
Benzoic acid 2-hydroxylase (BA2H) catalyzes the biosynthesis of salicylic from benzoic acid. The enzyme has been partially purified and characterized as a soluble protein 160 kDa. High-efficiency in vivo labeling with 18O2 suggested that BA2H is an oxygenase specifically hydroxylates ortho position was strongly induced by either tobacco mosaic virus inoculation or infiltration leaves it inhibited CO other inhibitors cytochrome P450 hydroxylases. activity immunodepleted antibodies raised...
Nitration of tyrosine (Y) residues proteins is a low abundant post-translational modification that modulates protein function or fate in animal systems. However, very little known about the vivo prevalence this and its corresponding targets plants. Immunoprecipitation, based on an anti-3-nitroY antibody, was performed to pull-down potential Y nitration Arabidopsis thaliana proteome. Further shotgun liquid chromatography-mass spectrometry (LC-MS/MS) proteomic analysis immunoprecipitated...
Abstract Salinity is one of the major limitations to wheat production worldwide. This study was designed evaluate level genetic variation among 150 internationally derived genotypes for salinity tolerance at germination, seedling and adult plant stages, with aim identifying new resources desirable adaptation characteristics breeding programmes further studies. In all growth genotype salt treatment effects were observed. Salt stress caused 33 %, 51 % 82 reductions in germination vigor, shoot...
Nitric oxide may limit ABA signaling in plants by rapid inactivation of the receptors through tyrosine nitration.
Recently, we described the ubiquitylation of PYL4 and PYR1 by RING E3 ubiquitin ligase RSL1 at plasma membrane Arabidopsis thaliana This suggested that ubiquitylated abscisic acid (ABA) receptors might be targeted to vacuolar degradation pathway because such is usually an internalization signal for endocytic route. Here, show FYVE1 (previously termed FREE1), a recently component endosomal sorting complex required transport (ESCRT) machinery, interacted with RSL1-receptor complexes recruited...
Wounded leaves of Arabidopsis thaliana show transient immunity to Botrytis cinerea, the causal agent grey mould. Using a fluorescent probe, histological staining and luminol assay, we now that reactive oxygen species (ROS), including H2O2 O2−, are produced within minutes after wounding. ROS formed in absence enzymes Atrboh D F can be prevented by diphenylene iodonium (DPI) or catalase. was shown protect plants upon exogenous application. accumulation resistance B. cinerea were abolished when...
Abstract The transition from etiolated to green seedlings involves a shift hypocotyl growth-promoting conditions growth restraint. These changes occur through complex light-driven process involving multiple and tightly coordinated hormonal signaling pathways. Nitric oxide (NO) has been lately characterized as regulator of plant development interacting with hormone signaling. Here, we show that Arabidopsis (Arabidopsis thaliana) NO-deficient mutant hypocotyls are longer than those wild-type...
Successful emergence from the soil is essential for plant establishment in natural and farmed systems. It has been assumed that absence of light preeminent signal perceived during early seedling development, leading to a distinct morphogenic plan (skotomorphogenesis) [1Wu S.H. Gene expression regulation photomorphogenesis perspective central dogma.Annu. Rev. Plant Biol. 2014; 65: 311-333Crossref PubMed Scopus (78) Google Scholar], characterized by traits providing an adaptive advantage until...
Plants are able to adapt the environment dynamically through regulation of their growth and development. Excess copper (Cu(2+)), a toxic heavy metal, induces morphological alterations in plant organs; however, underlying mechanisms still unclear. With this mind, multiple signalling functions nitric oxide (NO) cells its possible regulatory role relationship with auxin were examined during Cu(2+)-induced responses.Endogenous distribution was determined by microscopic observation X-Gluc-stained...
Abstract Plants are very plastic in adapting growth and development to changing adverse environmental conditions. This feature will be essential for plants survive climate changes characterized by extreme temperatures rainfall. Although require molecular oxygen (O2) live, they can overcome transient low-O2 conditions (hypoxia) until return standard 21% O2 atmospheric (normoxia). After heavy rainfall, submerged flooded lands undergo hypoxia water recedes normoxia is recovered. The accumulated...
Transcription factors (TFs) are key regulators of gene expression in all organisms. In eukaryotes, TFs often represented by functionally redundant members large families. Overexpression might prove a means to unveil the biological functions TFs; however, constitutive overexpression frequently causes severe developmental defects, preventing their functional characterization. Conditional strategies help overcome this problem. Here, we report on TRANSPLANTA collection Arabidopsis lines, each...