The apoptosis-inducing effect of fucoxanthin on human leukemic HL-60 cells was investigated. Fucoxanthin, which obtained from the brown alga Undaria pinnatifida, inhibited proliferation and induced DNA fragmentation agarose gel electrophoresis, a typical characteristic feature apoptotic cells. results sandwich ELISA using anti-biotin-antibody anti-DNA-antibody also demonstrated in accordance with increase concentration incubation time. Since level did not after 24 h incubation, appeared to...

Alzheimer's disease (AD) is a common neurodegenerative disorders, in which oxidative stress plays an important role. The present study investigated the effect of eicosapentaenoic acid-enriched phospholipids (EPA-enriched PL) from sea cucumber Cucumaria frondosa on injury PC12 cells induced by hydrogen peroxide (H2O2) and tert-butylhydroperoxide (t-BHP). We also studied EPA-enriched PL learning memory functions senescence-accelerated prone mouse strain 8 (SAMP8) vivo. Pretreatment with...

Summary We isolated Phe–Leu as an angiotensin I‐converting enzyme (ACE) inhibitor from hydrolysate of chum salmon muscle. The IC 50 value this peptide was 13.6 μ m , and it showed non‐competitive inhibition. reverse sequence dipeptide Leu–Phe also ACE inhibitory activity. However, is much less than with 383.2 . In addition, the inhibition mode competitive. To investigate relationship between properties, we further measured activity mechanism using six Trp‐containing dipeptides, which had...

Abstract In fishery industries, large amounts of byproducts are discarded during processing; therefore, their use as a source n‐3 polyunsaturated fatty acids (PUFAs) is beneficial. this study, we evaluated the potential anti‐allergic effect lysophosphatidylcholine (Lyso‐PtdCho) derived from squid skin phosphatidylcholine (PtdCho) by measuring production leukotriene (LT) B 4 and C , which chemical mediators produced mast cells in immediate allergic reactions. Squid Lyso‐PtdCho, with...

Two novel bromophenols (1 and 2) were isolated from an extract of a red alga, Odonthalia corymbifera, together with known bromophenol (3). The determined as 4-bromo-2, 3-dihydroxy-6-hydroxymethylphenyl 2, 5-dibromo-6-hydroxy-3-hydroxymethylphenyl ether (1) bis(2, 3-dibromo-4,5-dihydroxybenzyl) (2), spectroscopic evidence. Compounds 1-3 found to be inactivators alpha-glucosidase.

We studied changes in mechanical strength during postmortem aging of bovine semitendinosus muscle using intramuscular connective tissue (IMCT) preparations from stored at 4 degrees C. In the preparation, fiber elements were removed, leaving a virtually intact structure endomysium and perimysium. The shear-force value IMCT preparation remained unchanged up to 10 d decreased linearly thereafter. yield perimysial fraction, which was measured as an indicator properties perimysium, almost 14...

Polyunsaturated fatty acids (PUFAs) exhibit beneficial biological functions in carcinogenic processes. We examined the effects of PUFAs acid and phospholipid forms on three colon cancer cell lines (HT-29, Caco-2, DLD-1). Docosahexaenoic (DHA) eicosapentaenoic (EPA) both showed growth inhibition experimental lines. But these had strongest growth-inhibitory effect HT-29 than Caco-2 DLD-1. Combined application sodium butyrate (NaBt) increased inhibition. Growth was apparently caused by lipid...

Phospholipase D (PLD)-mediated transphosphatidylation of squid skin lecithin with L-serine was examined to prepare docosahexaenoic acid-containing phosphatidylserine (DHA-PS). When a biphasic system organic solvent and 0.2 M acetate buffer (pH 5.5) used, PS synthesis significantly affected by the amount 3.4 L-serine-containing buffer. L-Serine concentration in choice were also crucial. In typical reaction 0.8 unit PLD (Streptomyces sp.), 2.5 mL ethyl substrate solution containing 30 mg...

The present study was undertaken to examine the effect of cerebrosides derived from sea cucumber Acaudina molpadioides and starfish Asterias amurensis on anti-tumor activity in vitro vivo. results indicated that both (AMC) (AAC) exhibited an inhibitory cell proliferation through induction apoptosis S180 cells. Moreover, administration AMC AAC (50 mg/kg BW) tumor bearing mice reduced weight by 45.24 % 35.71 %, respectively. In ascites model, treatment a significant fluid growth inhibition...

Neurodegenerative disorders are a class of diseases that have been linked to apoptosis induced by elevated levels reactive oxygen species (ROS). The present study was undertaken explore the effect sea cucumber cerebrosides (SCC) and starfish (SFC) on hydrogen peroxide (H2O2) tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in PC12 cells. Cell viability, leakage lactate dehydrogenase (LDH), (ROS) level superoxide dismutase (SOD) activity were determined for their damage. Quantitative...

Mushroom (Agaricus blazei Murill) extract has been reported to possess antitumor effects through immune activation. Here, we investigated the beneficial of combining A. with marine phospholipids in comparison alone on myeloma sp2 tumor suppression when orally administrated. The experimental groups designed for bearing BALB/c nu/nu mice were drinks of: (1)control; (2)1.0 mg/mL squid phospholipid liposome alone; (3)0.5 Murill water (4)1.0 0.5 form those simple mixture; and (5)1.0 partially...

The objective of this study was to investigate the effect dietary supplementation with 5-aminolevulinic acid (5-ALA) on immune system, inflammatory response, and growth performance broiler chickens. levels cluster differentiation 3 (CD3) mRNA in spleens chickens gradually increased 5-ALA concentration, while expression interleukin (IL)-2 decreased. Mitogen-induced proliferation splenic mononuclear cells blood cell phagocytosis fed 0.001 0.01% 5-ALA-supplemented diets were significantly...

EPA-PL rescues the cancer-associated cachexia<italic>via</italic>inhibiting lipolysis.

1. Changes in plasma alpha 1-acid glycoprotein (AGP) concentration and immune responses following Escherichia coli lipopolysaccharide (LPS) injection were studied broiler chickens. 2. Higher AGP concentrations observed from 12 to 48 h after a single of LPS. 3. The highest was on day 2 followed by gradual decrease chicks injected with 150 micrograms/kg body weight LPS every for 13 d. 4. Plasma daily at 900 d increased 2, decreased 4 the found before injection. again 10. 5. interleukin-1...

Phospholipids are fascinating in terms of important bio-functional compounds. The present work investigated the effect polyunsaturated phosphatidylcholine (PC) and phosphatidylserine (PS) on butyrate-induced growth inhibition, differentiation apoptosis using Caco-2 cells. Growth inhibition cells became apparent 24 h after addition PC while it took 48 with PS. Alkaline phosphatase activity increased combined or PS sodium butyrate (NaBT) at 72 h, indicating that enhanced cell presence NaBT. An...

Five bromophenols isolated from three Rhodomelaceae algae (Laurencia nipponica, Polysiphonia morrowii, Odonthalia corymbifera) showed inhibitory effects against glucose 6-phosphate dehydrogenase (G6PD). Among them, the symmetric bromophenol dimer (5) highest activity G6PD.