A5068299078- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- Mesenchymal stem cell research
- Orthopaedic implants and arthroplasty
- Spine and Intervertebral Disc Pathology
- Osteoarthritis Treatment and Mechanisms
- Nerve injury and regeneration
- Collagen: Extraction and Characterization
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- biodegradable polymer synthesis and properties
- MicroRNA in disease regulation
- Spinal Fractures and Fixation Techniques
- Additive Manufacturing and 3D Printing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Bone health and treatments
- Knee injuries and reconstruction techniques
- Tissue Engineering and Regenerative Medicine
- Hippo pathway signaling and YAP/TAZ
- Silk-based biomaterials and applications
- Medical Imaging and Analysis
- Ion Channels and Receptors
- Neurogenesis and neuroplasticity mechanisms
- Spinal Cord Injury Research
Tokyo Medical and Dental University
2012-2025
Wayne State University
2014
Neurological Surgery
2014
Olympus (Japan)
2014
China Medical University
2014
First Hospital of China Medical University
2014
Capital Medical University
2014
Soka University
2010-2012
Spinal Research
2011
Japan Science and Technology Agency
2006
Abstract Articular cartilage has a limited capacity for self‐renewal. This article reports the development of porous hydroxyapatite/collagen (HAp/Col) scaffold as bone void filler and vehicle drug administration. The consists HAp nanocrystals type I atelocollagen. purpose this study was to investigate efficacy HAp/Col impregnated with FGF‐2 repair large osteochondral defects in rabbit model. Ninety‐six cylindrical 5 mm diameter depth were created femoral trochlear groove right knee. Animals...
We evaluated whether dexamethasone augments the osteogenic capability of bone marrow-derived stromal cells (BMSCs) and muscle tissue-derived (MuSCs), both which are thought to contribute ectopic formation induced by morphogenetic protein-2 (BMP-2), determined underlying mechanisms. Rat BMSCs MuSCs were cultured in growth media with or without 10-7 M then differentiated under conditions BMP-2. The effects on cell proliferation differentiation, also BMP-2, analyzed. Dexamethasone affected not...
Background: Flap necrosis remains a major complication of reconstructive surgery. To improve skin flap survival, various treatments with vasodilators, antiplatelet drugs, or the local administration growth factors have been performed. However, sufficient prevention is not well established. Platelet-rich plasma has used as an autologous factor and includes factors. The authors evaluated whether platelet-rich can survival in experimental rat model. Methods: Cranially based dorsal cutaneous...
Transplantation of aggregates synovial mesenchymal stem cells (MSCs) enhanced meniscus regeneration in rats. Anatomy and biological properties the depend on animal species. To apply this technique clinically, it is valuable to investigate use animals genetically close humans. We investigated whether transplantation autologous MSCs promoted meniscal aged primates. Chynomolgus primates between 12 13 years old were used. After anterior halves medial menisci both knees removed, an average 14...
Porous hydroxyapatite/collagen composite (HAp/Col) is a bioresorbable bone substitute composed of nano-scale HAp and porcine type 1 collagen. In this study, the efficacy safety were assessed in comparison to commercially available porous β-tricalcium phosphate (β-TCP).Patients with defects caused by benign tumors, fractures, or harvesting autografts randomly allocated for implantation HAp/Col (n = 63) β-TCP 63). X-ray images scored used evaluate until 24 weeks after surgery. Blood tests...
Abstract This study investigated the effects of three‐dimensional (3‐D) pore structure a porous hydroxyapatite/collagen (HAp/Col) composite on their mechanical properties and in vivo tissue ingrowth. The unique 3‐D structure, comprising unidirectionally interconnected pores, was fabricated by unidirectional growth ice crystals using cooling stage subsequent freeze‐drying process. pores had spindle‐shaped cross section, size gradually increased from bottom to upper face. showed an elastic...
Gene therapy for neuropathic pain requires efficient gene delivery to both central and peripheral nervous systems. We previously showed that an adenoassociated virus serotype 9 (AAV9) vector expressing short-hairpin RNA (shRNA) could suppress target molecule expression in the dorsal root ganglia (DRG) spinal cord upon intrathecal injection. To evaluate therapeutic potential of this approach, we constructed AAV9 encoding shRNA against vanilloid receptor 1 (TRPV1), which is important acute...
A prospective, comparative study.We developed a hybrid graft (HBG) of porous β-tricalcium phosphate ceramics/percutaneously harvested bone sticks/autologous marrow aspirate for lumbar posterolateral fusion (PLF). The aim this study was to investigate the efficacy HBG as substitute conventional corticocancellous iliac autografts.Iliac crest (ICBG) has been traditionally used golden standard spinal fusion. significant complication rate associated with harvesting ICBG, however, encouraged...
Abstract Articular cartilage has a limited capacity for spontaneous repair, and an effective method to repair damaged articular not yet been established. The purpose of this study was evaluate the effect transplantation porous hydroxyapatite collagen ( HAp / C ol) impregnated with bone morphogenetic protein‐2 BMP ‐2). To characteristics ol as drug delivery carrier recombinant human ‐2 rhBMP ‐2), adsorption release kinetics were analyzed. Porous different amounts (0, 5, 25 μg) implanted into...
Abstract There are two types of carbonated apatite (cap): A‐type and B‐type, where carbonate ions substitute for hydroxyl sites in A‐CAp phosphate B‐CAp. Despite the known capacity CAp supporting activities bone cells, distinct potential B‐CAp as porous grafts is unclear. This study aimed to fabricate evaluate their biological vitro vivo compared stoichiometric hydroxyapatite (HAp). Gelatin‐casting freeze‐drying followed by sintering were used produce A‐ with high porosity ∼70%, pore sizes...
In the last decade, researchers and clinicians have reported that transplantation of bone marrow stromal cells (BMSCs) promotes functional recovery after brain or spinal cord injury (SCI). However, an appropriate scaffold designed for injured is needed to enhance survival transplanted BMSCs promote nerve regeneration. We previously tested a honeycomb collagen sponge (HC), which when applied transected allowed bridging gap with fibers. this study, we examined whether HC implant combined rat...
A three-dimensional porous hydroxyapatite/collagen (HAp/Col) composite with a random pore structure was fabricated using freeze-drying processes; the self-organized HAp/Col nanocomposite weight ratio of 80.5:19.5, freeze-dried, kneaded in 100 mM sodium phosphate buffer, frozen at −20°C and freeze-dried. The cross-linkage Col molecules introduced dehydrothermally 140°C vacuo. had porosity 94.7% sizes between 200 500 μm. compressive stress for wet buffer saline (PBS) gradually decreased during...
Reconstruction of large bone defects is a great challenge in orthopedic research. In the present study, we prepared composites marrow-derived stromal cells (BMSCs) and β-tricalcium phosphate (β-TCP) with three novel aspects: proliferation BMSCs continuous dexamethasone treatment, cell loading under low pressure, use autologous plasma as medium. The effectiveness resulting composite for bone-defect reconstruction was tested non-human primate model, union capability regenerated bones...
Recent advances in tissue engineering techniques have allowed porous biomaterials to be combined with osteogenic cells for effective bone regeneration. We developed a simple low-pressure cell-loading method using only syringes and stopcocks, examined the effect of this on osteogenesis when applied combination highly beta-tricalcium phosphate (beta-TCP) fresh autologous marrow. Both block granule beta-TCP scaffolds were used prepare implants three different ways: without marrow as control,...
Abstract In this study, the effects of gamma‐ray irradiation on mechanical properties, absorbability, and osteoconductivity porous hydroxyapatite/collagen (HAp/Col) were investigated. Porous HAp/Col was exposed to 16, 25, 35, or 50 kGy irradiation. The compressive elastic modulus showed dose‐dependence, with a particularly pronounced decrease in 50‐kGy treatment group. An vitro enzymatic digestion test that resulted accelerated degradation by collagenase. For vivo studies, transplanted into...
Osteomyelitis remains one of the most challenging disorders for orthopedic doctors despite advancement therapeutic techniques. The purpose this study was to investigate feasibility local antibiotic administration using hydroxyapatite/collagen (HAp/Col) as a drug delivery system. We hypothesized that higher adsorbability antibiotics onto HAp/Col will result in more efficacious activity and therefore, treatment osteomyelitis. Eight were examined study: amikacin, cefazolin, cefotiam,...