A5005397460- Digital Holography and Microscopy
- Spectroscopy Techniques in Biomedical and Chemical Research
- Optical Coherence Tomography Applications
- Alkaloids: synthesis and pharmacology
- 3D Printing in Biomedical Research
- Optical measurement and interference techniques
- Advanced Fluorescence Microscopy Techniques
- Electrical and Bioimpedance Tomography
- Phytochemistry and Biological Activities
- Advanced X-ray Imaging Techniques
- Cell Image Analysis Techniques
- Fern and Epiphyte Biology
- Bacterial Identification and Susceptibility Testing
- Thermal and Kinetic Analysis
- Laser-induced spectroscopy and plasma
- Image Processing Techniques and Applications
- CAR-T cell therapy research
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Energetic Materials and Combustion
- Laser Material Processing Techniques
- Laser Design and Applications
- Luminescence Properties of Advanced Materials
- Carbon and Quantum Dots Applications
- Chemical Thermodynamics and Molecular Structure
Warsaw University of Technology
2021-2024
National Research Tomsk State University
2019
Tomsk Polytechnic University
2019
Yokukansan (YKS) is a traditional Japanese herbal medicine that increasingly being studied for its effects on neurodegenerative diseases. In our study, we presented novel methodology multimodal analysis of the YKS nerve cells. The measurements 3D refractive index distribution and changes performed by holographic tomography were supported with an investigation Raman micro-spectroscopy fluorescence microscopy to gather complementary morphological chemical information about cells influence. It...
Low-level laser therapy (LLLT) is a therapeutic tool that uses the photobiochemical interaction between light and tissue. Its effectiveness controversial due to strong dependence on dosimetric parameters. In this work, we demonstrate digital holographic microscopy an effective label-free imaging technique analyze effects of LLLT biological cells, propose full methodology create correct synthetic aperture phase maps for further extensive, highly accurate statistical analysis. The proposed has...
Three-dimensional, quantitative imaging of biological cells and their internal structures performed by optical diffraction tomography (ODT) is an important part biomedical research. However, conducting analysis ODT images requires performing 3D segmentation with high accuracy, often unattainable available methods. Therefore, in this work, we present a new semi-automatic method, called ODT-SAS, which combines several non-machine-learning techniques to segment 2 types organelles: nucleoli...
The approach utilizing digital holographic microscopy (DHM) for analysing the status of T cells activation, important part CAR immunotherapy, is presented. preliminary research reveals usability DHM in this field.
In this work we present an interferometric approach to quantifying the bacterial growth medium diffusion through a biofilm pretreated with tested antibacterial agent. The effect of agents on Pseudomonas aeruginosa PAO1 mature was by digital holographic microscopy and laser interferometry compared biological tests (spectrofluorimetric measurement pyocyanin pyoverdine production). It is shown that multimodal provides better determination cell eradication and/or degradation matrix forming biofilm.
Transmission optical coherence tomography (tOCT) is gaining interest for imaging biological samples; however, so far, quantitative phase information has not been directly obtained. This study introduces SOPHAST-OCT, an approach integrated 2D recovery in Off-Axis Full-Field Swept-Source tOCT. It uses phase-shifting to leverage OCT’s inherent changes between recorded interferograms. SOPHAST-OCT demonstrates promising agreement with the simulated ground truth phase. represents a first step...
Transmission optical coherence tomography (OCT) enables analysis of biological specimens in vitro through detection forward scattered light. Up to now, transmission OCT was considered as a technique that cannot directly retrieve quantitative phase and is thus qualitative method. In this paper, we present qtOCT, novel Unlike existing approaches, qtOCT allows for direct, easy, fast rigorous retrieval 2D integrated information from full-field swept-source measurements. Our method based on...
Tomographic phase microscopy (TPM) is one of the most widely used quantitative imaging techniques. It a non-invasive, label-free technique that allows high-resolution imaging. enables morphology living cell to be captured without chemical treatment, by using refractive index (RI) as contrast parameter image internal structures. In this paper, we demonstrate due fact biological cells are highly heterogeneous structures, it crucial use full volumetric data calculate average RI values samples...
Bortezomib is one of the most researched proteasome inhibitor drug in cancer cell research. Studying its effects, measuring and monitoring treatment response effectiveness a widely developing area The introduction non-invasive measurement tools into this research very important desirable development, as it promising alternative to existing chemical tests. In our work we presented multimodal methodology connecting multiple label-free techniques study effects bortezomib on RPMI8226 cells. We...
The spectral, kinetic, spatial, and amplitude characteristics of emissions pressed samples PETN (pentaerythritole tetranitrate) by the first harmonic a neodymium laser (1064 nm, 12 ns) are studied. It is found that at moment irradiation pulses, hot spots formed on surface samples. energy density radiation varies in range 0.5–3 J/cm2. determined absorbing inhomogeneities parameters radiation. Multi-pulse excitation leads to annealing irregularities increased threshold optical breakdown....
The results of quantitative, label-free measurements SHSY-5Y cells under Low-Level Laser Therapy treatment are presented. time-lapse investigations performed by means digital holographic microscopy focused on dry mass density changes after light exposure.