A5020839545- Microfluidic and Bio-sensing Technologies
- Micro and Nano Robotics
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Bacterial biofilms and quorum sensing
- Microfluidic and Capillary Electrophoresis Applications
- Microbial Inactivation Methods
- Advanced Polymer Synthesis and Characterization
- DNA Repair Mechanisms
- Lipid Membrane Structure and Behavior
- Pickering emulsions and particle stabilization
- Cell Image Analysis Techniques
- Microbial Metabolism and Applications
- Polymer Surface Interaction Studies
- Click Chemistry and Applications
- Single-cell and spatial transcriptomics
- Medicinal Plants and Neuroprotection
- Scientific Computing and Data Management
- Advanced biosensing and bioanalysis techniques
- Electrohydrodynamics and Fluid Dynamics
University of Freiburg
2022
Swiss Nanoscience Institute
2019
University of Basel
2013-2019
Center for NanoScience
2019
Bacterial surface attachment is mediated by filamentous appendages called pili. Here, we describe the role of Tad pili during colonization Caulobacter crescentus Using an optical trap and microfluidic controlled flow conditions to mimic natural environments, demonstrated that undergo repeated dynamic cycles extension retraction. Within seconds after establishing contact, pilus retraction reorients cells into upright position, promoting walking-like movements against medium flow....
The ATRP-catalyzing enzyme horseradish peroxidase was encapsulated into the protein cage thermosome resulting in an all-protein nanoreactor system for controlled radical polymerizations.
The stochastic nature of biological systems makes the study individual cells a necessity in biology. Yet, handling and disruption single analysis relatively low concentrations their protein components still challenges available techniques. Transmission electron microscopy (TEM) allows for proteins at single-molecule level. Here, we present system single-cell lysis under light observation, followed by rapid uptake cell lysate. Eukaryotic were grown on conductively coated glass slides observed...
Abstract Seeing physiological processes at the nanoscale in living organisms without labeling is an ultimate goal life sciences. Using X-ray ptychography, we explored situ dynamics of unstained, fission yeast Schizosaccharomyces pombe cells natural, aqueous environment nanoscale. In contrast to previous imaging studies on biological matter, this work eukaryotic were alive even after several ptychographic scans, which allowed us visualize chromatin motion as well autophagic cell death induced...
On-chip lysis is required in many lab-on-chip applications involving cell studies.
Abstract Background New experimental methods must be developed to study interaction networks in systems biology. To reduce biological noise, individual subjects, such as single cells, should analyzed using high throughput approaches. The measurement of several correlative physical properties would further improve data consistency. Accordingly, a considerable quantity acquired, correlated, catalogued and stored database for subsequent analysis. Results We have openBEB (open Biological...
ABSTRACT Bacterial surface attachment is mediated by rotary flagella and filamentous appendages called pili. Here, we describe the role of Tad pili during colonization Caulobacter crescentus . Using an optical trap microfluidic controlled flow conditions as a mimic natural environments, demonstrate that undergo repeated cycles extension retraction. Within seconds after establishing contact, reorient cells into upright position promoting walking-like movements against medium flow....
Abstract Surface attachment of bacteria is the first step biofilm formation and often mediated coordinated by extracellular appendages, flagellum pili. The model organism Caulobacter crescentus undergoes an asymmetric division cycle, giving rise to a motile “swarmer cell” sessile “stalked cell”, which attached surface. In highly polarized predivisional cell, pili flagellum, are assembled at pole opposite stalk, both activated before during process cell separation. We explored interplay...