A5102887436- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Neurological disorders and treatments
- Amyotrophic Lateral Sclerosis Research
- Dementia and Cognitive Impairment Research
- Cerebrospinal fluid and hydrocephalus
- Neurogenetic and Muscular Disorders Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Prion Diseases and Protein Misfolding
- Genetic Neurodegenerative Diseases
- Botulinum Toxin and Related Neurological Disorders
- Nuclear Receptors and Signaling
- Trace Elements in Health
- Cholinesterase and Neurodegenerative Diseases
- Cellular transport and secretion
- Sarcoidosis and Beryllium Toxicity Research
- Intracerebral and Subarachnoid Hemorrhage Research
- Fetal and Pediatric Neurological Disorders
- Cerebrovascular and genetic disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA regulation and disease
- Spinal Dysraphism and Malformations
- Computational Drug Discovery Methods
- Functional Brain Connectivity Studies
National Institutes for Quantum Science and Technology
2020-2025
Kyoto Prefectural University of Medicine
2014-2024
Jikei University School of Medicine
2024
John Wiley & Sons (United States)
2024
Hudson Institute
2024
University Hospital Kyoto Prefectural University of Medicine
2023
Boston University
2021
Japan Agency for Medical Research and Development
2016-2020
Chiba Hospital
2020
Lancaster University
2020
Among the various disorders manifesting dementia, gait disturbance, and urinary incontinence in elderly population, idiopathic normal pressure hydrocephalus (iNPH) is becoming of great importance. After publication first edition Guidelines for Management Idiopathic Normal Pressure Hydrocephalus 2004 (the English version was published 2008), clinical awareness iNPH has risen dramatically, number shunt surgeries increased rapidly across Japan. Clinical basic research on significantly, more...
To date, there is no accepted clinical diagnostic test for Parkinson disease (PD) that based on biochemical analysis of blood or CSF. The discovery mutations in the SNCA gene encoding α-synuclein familial parkinsonism and accumulation PD brain suggested a critical role this protein etiology.We investigated total oligomers levels CSF from patients clinically diagnosed with PD, progressive supranuclear palsy (PSP), Alzheimer (AD), age-matched controls, using ELISA developed our laboratory.The...
Parkinson disease (PD) is a highly prevalent and incurable neurodegenerative associated with the accumulation of misfolded α-synuclein (αSyn) aggregates. An important problem in this lack sensitive, specific, noninvasive biochemical diagnosis to help clinical evaluation, monitoring progression, early differential from related diseases.To develop novel assay high sensitivity specificity detect small quantities αSyn aggregates circulating cerebrospinal fluid (CSF) patients affected by PD...
Among the various disorders that manifest with gait disturbance, cognitive impairment, and urinary incontinence in elderly population, idiopathic normal pressure hydrocephalus (iNPH) is becoming of great importance. The first edition these guidelines for management iNPH was published 2004, second 2012, to provide a series timely, evidence-based recommendations related iNPH. Since last edition, clinical awareness has risen dramatically, basic research efforts on have increased significantly....
There is accumulating evidence that soluble amyloid-beta (Abeta) oligomers, rather than amyloid fibrils, are the principal pathogenic species in Alzheimer disease (AD). Here, we have developed a novel enzyme-linked immunosorbent assay (ELISA) specific for high-molecular-weight (HMW) Abeta oligomers. Analysis of oligomers derived from synthetic 1-42, by size-exclusion chromatography (SEC), revealed our ELISA specifically detected HMW 40-200 kDa. Using this ELISA, significantly higher...
There is still a substantial unmet need for less invasive and lower-cost blood-based biomarkers to detect brain Alzheimer's disease (AD) pathology. This study aimed determine whether quantification of plasma tau phosphorylated at threonine 181 (p-tau181) informative in the diagnosis AD. We have developed novel ultrasensitive immunoassay quantify p-tau181, measured levels p-tau181 three cohorts. In first cohort composed 20 AD patients 15 age-matched controls, were significantly higher than...
Despite decades of intensive research, to date, there is no accepted diagnosis for Parkinson's disease (PD) based on biochemical analysis blood or CSF. However, neurodegeneration in the brains PD patients begins several years before manifestation clinical symptoms, pointing serious flaw/limitations this approach. To explore potential use alpha-synuclein (α-syn) species as candidate biomarkers PD, we generated specific antibodies directed against wide array α-syn species, namely total-,...
The inheritance of the apolipoprotein E (apoE) epsilon4 allele is a prevailing risk factor for sporadic and familial Alzheimer's disease (AD). ApoE isoforms bind directly to amyloid beta (Abeta) peptides both in vitro vivo. Recent studies suggest that association apoE with lipids may modulate its interaction Abeta. We examined binding lipid-associated delipidated apoE3 apoE4 Abeta utilizing solid-phase assay estimated dissociation constants various species. Using native from stably...
<h3>Background</h3> The clinical diagnosis of dementia with Lewy bodies (DLB) is made on the basis consensus criteria; however, sensitivity criteria relatively low. There are no generally accepted biomarkers to distinguish DLB from other dementias. Here utility quantification α-synuclein, β-amyloid42 (Aβ42) and tau in CSF patients DLB, Alzheimer9s disease (AD) dementias was examined. <h3>Methods</h3> 86 were divided into three age sex matched groups: (n=34), AD (n=31) (n=21). Two α-synuclein...
Background and Purpose Dementia with Lewy bodies (DLB) needs to be distinguished from Alzheimer’s disease (AD) because of important differences in patient management outcome. Severe cardiac sympathetic degeneration occurs DLB, but not AD, offering a potential system for biological diagnostic marker. The primary aim this study was investigate the accuracy, ante-mortem differentiation probable DLB imaging ligand 123I-meta-iodobenzylguanidine (MIBG) which binds noradrenaline reuptake site,...
ABSTRACT Background Parkinson's disease (PD) diagnosis is mainly based on clinical criteria, with a high risk of misdiagnosis. The identification reliable biomarkers for and progression has key role developing disease‐modifying therapies. In this article, we investigated the longitudinal changes CSF α‐synuclein species in early PD patients explored potential use these as surrogate progression. Methods We used our newly developed enzyme‐linked immunosorbent assay systems measuring different...
Cerebral amyloid angiopathy is commonly associated with normal aging and Alzheimer's disease it also the principal feature of hereditary cerebral hemorrhage amyloidosis Dutch type, a familial condition to point mutation G C at codon 693 β (Aβ) precursor protein gene resulting in Glu Gln substitution position 22 Aβ (E22Q). The patients carrying AβE22Q variant usually present lobar hemorrhages before 50 years age. A different described several members three Italian kindred who presented...
TAR DNA binding protein of 43 kDa (TDP-43) is likely to be the major pathogenetic in amyotrophic lateral sclerosis (ALS). A previous study has shown that levels TDP-43 CSF measured by an ELISA are significantly higher for ALS patients than controls. The aim this was investigate whether elevated specific ALS, and associated with clinical profiles patients. We same 27 50 neurodegenerative or inflammatory disease controls such as Parkinson's disease, multiple sclerosis, Guillain-Barré syndrome.
Abstract Objectives There have been no nationwide epidemiological studies of idiopathic normal pressure hydrocephalus ( iNPH ) in Japan. Therefore, a epidemiologic survey was performed to determine the number cases and clinical characteristics by sex diagnostic level. Methods The first examined numbers that met criteria those who underwent shunt operations 2012. second gathered patients' details clarify their background characteristics. Results estimated meeting 2012 12,900, with 6,700...
α-Synuclein is the main component of Lewy bodies, intraneuronal inclusion bodies characteristic Parkinson's disease. Although α-synuclein accumulation caused by inhibition proteasome and autophagy-lysosome, degradation inclusions still unknown. Formation body-like can be replicated in cultured cells introducing fibrils generated vitro. We used this cell culture model to investigate autophagy impaired mitochondria. The intracellular immediately underwent phosphorylation ubiquitination....
Alzheimer's disease (AD) is the most dominant neurodegenerative disorder that causes dementia, and no effective treatments are available. To study its pathogenesis develop therapeutics, animal models representing pathologies needed. Although many species senile plaques (SP) composed of amyloid-β (Aβ) proteins identical to those found in humans, none them exhibit neurofibrillary tangles (NFT) subsequent neurodegeneration, which integral parts pathology AD. The present shows Aβ accumulation,...
Chronic Traumatic Encephalopathy (CTE) is a tauopathy that affects individuals with history of repetitive mild traumatic brain injury, such as American football players.Initial neuropathologic changes in CTE include perivascular deposition phosphorylated microtubule-associated protein tau (p-tau) neurofibrillary tangles and other aggregates neurons, astrocytes cell processes an irregular pattern often at the depths cortical sulci.In later stages, p-tau depositions become widespread...