A5101781347- Pain Mechanisms and Treatments
- Diabetes Treatment and Management
- Botulinum Toxin and Related Neurological Disorders
- Alzheimer's disease research and treatments
- Nerve injury and regeneration
- Parkinson's Disease Mechanisms and Treatments
- Mesenchymal stem cell research
- Additive Manufacturing Materials and Processes
- Advanced Glycation End Products research
- Additive Manufacturing and 3D Printing Technologies
- VLSI and FPGA Design Techniques
- Low-power high-performance VLSI design
- Cerebrovascular and genetic disorders
- Moyamoya disease diagnosis and treatment
- Advancements in Semiconductor Devices and Circuit Design
- Pharmacology and Obesity Treatment
- Biochemical effects in animals
- Pancreatic function and diabetes
- Dementia and Cognitive Impairment Research
- Retinal Diseases and Treatments
- Neurological Disorders and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Diabetes Management and Research
- Wound Healing and Treatments
- Apelin-related biomedical research
Kyoto Prefectural University of Medicine
2012-2025
Kyoto Prefectural Education Center
2024-2025
Aichi Medical University
2015-2024
San Francisco VA Medical Center
2024
University of California, San Francisco
2024
Nagoya University
2008-2023
University Hospital Kyoto Prefectural University of Medicine
2019-2023
National Cancer Centre Japan
2023
Chiba Cancer Center
2023
Kobe Central Hospital
2023
There is accumulating evidence that soluble amyloid-beta (Abeta) oligomers, rather than amyloid fibrils, are the principal pathogenic species in Alzheimer disease (AD). Here, we have developed a novel enzyme-linked immunosorbent assay (ELISA) specific for high-molecular-weight (HMW) Abeta oligomers. Analysis of oligomers derived from synthetic 1-42, by size-exclusion chromatography (SEC), revealed our ELISA specifically detected HMW 40-200 kDa. Using this ELISA, significantly higher...
There is still a substantial unmet need for less invasive and lower-cost blood-based biomarkers to detect brain Alzheimer's disease (AD) pathology. This study aimed determine whether quantification of plasma tau phosphorylated at threonine 181 (p-tau181) informative in the diagnosis AD. We have developed novel ultrasensitive immunoassay quantify p-tau181, measured levels p-tau181 three cohorts. In first cohort composed 20 AD patients 15 age-matched controls, were significantly higher than...
OBJECTIVE—Mesenchymal stem cells (MSCs) have been reported to secrete various cytokines that exhibit angiogenic and neurosupportive effects. This study was conducted investigate the effects of MSC transplantation on diabetic polyneuropathy (DPN) in rats. RESEARCH DESIGN AND METHODS—MSCs were isolated from bone marrow adult rats transplanted into hind limb skeletal muscles with an 8-week duration streptozotocin (STZ)-induced diabetes or age-matched normal by unilateral intramuscular...
<h3>Background</h3> Supported by compelling genetic data regarding early-onset familial Alzheimer disease (AD), the amyloid β-peptide (Aβ)–centric theory holds that Aβ is involved in pathogenesis of sporadic AD. Mutations precursor protein (<i>APP</i>), presenilin 1 (<i>PSEN1</i>), and 2 (<i>PSEN2</i>) genes lead to increased levels before symptoms arise. <h3>Objectives</h3> To evaluate pattern Pittsburgh Compound B (PiB) retention subjects with different autosomal dominant mutations...
We present a case of 32-year-old diabetic woman with Prader-Willi syndrome who developed severe ketoacidosis caused by sodium-glucose cotransporter 2 (SGLT2) inhibitor, novel class antihyperglycemic agents, during strict low-carbohydrate diet. At admission, serum glucose level 191 mg/dL was relatively low, though laboratory evaluations showed ketoacidosis. This is the first report SGLT2 inhibitor. It necessary to not only pay attention when using inhibitor in patients following diet, but...
OBJECTIVE The therapeutic potential of exendin-4, an agonist the glucagon-like peptide-1 receptor (GLP-1R), on diabetic polyneuropathy (DPN) in streptozotocin (STZ)-induced mice was investigated. RESEARCH DESIGN AND METHODS presence GLP-1R lumbar dorsal root ganglion (DRG) evaluated by immunohistochemical analyses. DRG neurons were dissected from C57BL6/J and cultured with or without Schwann cell–conditioned media absence GLP-1 (7–37) exendin-4. Then neurite outgrowth determined. In...
Relief from painful diabetic neuropathy is an important clinical issue. We have previously shown that the transplantation of cultured endothelial progenitor cells or mesenchymal stem ameliorated in rats. In this study, we investigated whether freshly isolated bone marrow-derived mononuclear (BM-MNCs) alleviates neuropathic pain early stage streptozotocin-induced Two weeks after STZ injection, BM-MNCs vehicle saline were injected into unilateral hind limb muscles. Mechanical hyperalgesia and...
Surrogate markers for the Alzheimer disease (AD)-associated 42-amino acid form of amyloid-beta (Abeta42) have been sought because they may aid in diagnosis AD and clarification pathogenesis. Here, we demonstrate that human cerebrospinal fluid (CSF) contains three APLP1-derived Abeta-like peptides (APL1beta) are generated by beta- gamma-cleavages at a concentration approximately 4.5 nM. These novel peptides, APL1beta25, APL1beta27 APL1beta28, were not deposited brains. Interestingly, most...
Recent studies advocate that omega-3 polyunsaturated fatty acids (ω-3 PUFAs) have direct anti-oxidative and anti-inflammatory effects in the vasculature; however, role of ω-3 PUFAs Schwann cells remains undetermined.Immortalized mouse (IMS32) were incubated with docosahexaenoic acid (DHA) eicosapentaenoic (EPA). The messenger ribonucleic levels several anti-oxidant enzymes (heme oxygenase-1 [Ho-1], nicotinamide adenine dinucleotide [phosphate] H quinone oxidoreductase 1, catalase, superoxide...
Accumulating data suggest that new cardiomyocytes in adults are generated from existing throughout life. To enhance the endogenous cardiac regeneration, we performed chemical screenings to identify compounds activate pro-proliferative YES-associated protein and transcriptional enhancer factor domain activities cardiomyocytes. We synthesized a novel fluorine-containing TT-10 (C11H10FN3OS2) biologically hit compound. promoted cardiomyocyte proliferation simultaneously exerted antioxidant...
Abstract Cancer cells employ glycolysis for their survival and growth (the “Warburg effect”). Consequently, surrounding including immune in the tumor microenvironment (TME) are exposed to hypoglycemic, hypoxic, low pH circumstances. Since effector T depend on functions, metabolically harsh TME established by cancer is unfavorable, resulting impairment of effective antitumor responses. By contrast, immunosuppressive such as regulatory (Treg) can infiltrate, proliferate, survive, exert...
Impaired vascularity and nerve degeneration are the most important pathophysiological abnormalities of diabetic polyneuropathy (DPN). Therefore, regeneration both vascular nervous systems is required for treatment DPN. The neural crest (NC) a transient embryonic structure in vertebrates that differentiates into vast range cells, including peripheral neurons, Schwann smooth muscle cells. In this study, we investigated ability transplantation NC-like (NCL) cells derived from aged mouse induced...
Down syndrome (DS) is the most prevalent chromosomal abnormality. Early-onset dementia with pathology of Alzheimer's disease (AD) frequently develops in DS. Reliable blood biomarkers are needed to support diagnosis for DS, since positron emission tomography or cerebrospinal fluid sampling burdensome, particularly patients Plasma t-tau one established AD, suggesting potential value as a biomarker The aim this study was assess and compare plasma levels adults DS an age-matched control...
Increased low-density lipoprotein levels are risk factors for diabetic neuropathy. Diabetes mellitus is associated with elevated metabolic stress, leading to oxidised formation. Therefore, it important investigate the mechanisms underlying pathogenesis of neuropathy in diabetes complicated by dyslipidaemia increased lipoprotein. Here, we examined effects hyperglycaemia and treatment on Schwann cell death its mechanisms. Immortalised mouse cells were treated under normo- or hyperglycaemic...
Background . Although pathological involvements of diabetic polyneuropathy (DPN) have been reported, no dependable treatment DPN has achieved. Recent studies shown that mesenchymal stem cells (MSCs) ameliorate DPN. Here we demonstrate a differentiation induced pluripotent (iPSCs) into MSC-like and investigate the therapeutic potential cell transplantation on Research Design Methods For induction cells, GFP-expressing iPSCs were cultured with retinoic acid, followed by adherent culture for 4...
Objective and Methods An SGLT2 inhibitor (ipragliflozin, dapagliflozin, luseogliflozin, tofogliflozin, or canagliflozin) was administered to 132 outpatients with type 2 diabetes mellitus without other antidiabetic drugs for 6 months evaluate its efficacy, the incidence of adverse events, influence on renal function.
Diabetic polyneuropathy (DPN) is one of the most prevalent diabetic complications. We previously demonstrated that exendin-4 (Ex4), a glucagon-like peptide-1 receptor agonist (GLP-1RA), has beneficial effects in animal models DPN. hypothesized GLP-1 signaling would protect neurons peripheral nervous system from oxidative insult Here, therapeutic potential GLP-1RAs on DPN was investigated depth using cellular model applied to dorsal root ganglion (DRG) neuronal cell line. Immortalized DRG...
Although nerve conduction study (NCS) using a standard electromyography system (EMGS) is considered to be the gold in evaluating diabetic polyneuropathy, this examination requires expensive equipment and well-trained technicians. We aimed validate point-of-care device, NC-stat/DPNCheck™, that has been developed for widespread use of NCS polyneuropathy.Diabetes patients underwent two kinds NCS: DPNCheck™ system. Inter-/intrarater reliability were also determined by intraclass correlation...
People with Down syndrome (DS) are at high risk of developing Alzheimer disease (AD) aging. The diagnosis and treatment trials hampered by a lack reliable blood biomarkers. Plasma neurofilament light chain (NfL) is one the established biomarkers AD, suggesting that it may be useful as an indicator dementia in DS patients. aims this study were: 1) to examine whether plasma levels NfL patients correlated decreased adaptive behavior scores year after sample collection, 2) compare adults...
Abstract Background Tako-tsubo syndrome(TTS) is a rarely reported complication of catheter ablation(CA) for atrial fibrillation(AF). Purpose To investigate the risk factors and characteristics patients who develop TTS after CA AF. Methods 481 consecutive cases underwent initial or multiple AF between May 2018 March 2024 were retrospectively studied. Pulmonary vein isolation(PVI) consisted point-by-point radiofrequency(RF) using 3.5 mm irrigated with contact force, cryoballoon(CB). All...