A5076689609- Nerve injury and regeneration
- Pain Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Lysosomal Storage Disorders Research
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Signaling Pathways in Disease
- Galectins and Cancer Biology
- Neurological Disorders and Treatments
- Hereditary Neurological Disorders
- Neuropeptides and Animal Physiology
- Aldose Reductase and Taurine
- Biochemical effects in animals
- Advanced Glycation End Products research
- Proteoglycans and glycosaminoglycans research
- Trypanosoma species research and implications
- Botulinum Toxin and Related Neurological Disorders
- Axon Guidance and Neuronal Signaling
- Carbohydrate Chemistry and Synthesis
- Calpain Protease Function and Regulation
- Diabetes Treatment and Management
- Autophagy in Disease and Therapy
- Adipose Tissue and Metabolism
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
Tokyo Metropolitan Institute of Medical Science
2016-2025
RELX Group (United States)
2022
Toyota Central Research and Development Laboratories (Japan)
2019-2021
Niigata Institute of Technology
2018-2021
Hirosaki University
2018-2019
Kyushu University
2016
Maebashi Institute of Technology
2016
Shahid Beheshti University of Medical Sciences
2012
Tokyo Metropolitan Police Department
2011
Tokyo Institute of Psychiatry
2009-2010
Abstract Autophagy, an evolutionarily conserved cytoplasmic degradation system, has been implicated as a convergent mechanism in various longevity pathways. Autophagic activity decreases with age several organisms, but the underlying is unclear. Here, we show that expression of Rubicon, negative regulator autophagy, increases aged worm, fly and mouse tissues at transcript and/or protein levels, suggesting age-dependent increase Rubicon impairs autophagy over time, thereby curtails animal...
The involvement of reactive oxygen species (ROS) in an augmented sensitivity to painful stimuli (hyperalgesia) during inflammation has been suggested, yet how and where ROS affect the pain signaling remain unknown. Here we report a novel role for superoxide-generating NADPH oxidase development hyperalgesia. In mice lacking Nox1 ( −/ Y ), catalytic subunit oxidase, thermal mechanical hyperalgesia was significantly attenuated, whereas no change nociceptive responses heat or observed. dorsal...
Inherited defects in the degradation of glycosphingolipids (GSLs) cause a group severe diseases known as GSL storage disorders. There are currently no effective treatments for majority these We have explored new treatment paradigm, substrate deprivation therapy, by constructing genetic model mice. Sandhoff's disease mice, which abnormally accumulate GSLs, were bred with mice that blocked their synthesis GSLs. The simultaneous and longer accumulated had improved neurologic function, much life...
Various neurotransmitters, such as dopamine, stimulate adenylyl cyclase to produce cAMP, which regulates neuronal functions. Genetic disruption of the type 5 isoform led a major loss activity in striatum-specific manner with small increase expression few other isoforms. D1 dopaminergic agonist-stimulated was attenuated, and this accompanied by decrease receptor Gsα. D2 agonist-mediated inhibition also blunted. Type cyclase-null mice exhibited Parkinsonian-like motor dysfunction, i.e....
Various neurotrophic factors that promote axonal regeneration have been investigated in vivo, but the signals prompt neurons to send out processes peripheral nerves after axotomy are not well understood. Previously, we shown oxidized galectin-1 (GAL-1/Ox) promotes initial growth nerves. However, mechanism by which GAL-1/Ox remains unclear and is subject of present study. To identify possible target cells GAL-1/Ox, a fluorescently labeled recombinant human (rhGAL-1/Ox) was incubated with DRG...
Although previous reports have revealed the therapeutic potential of stem cell transplantation in diabetic polyneuropathy, effects on long-term polyneuropathy not been investigated. In this study, we investigated whether dental pulp cells (DPSCs) ameliorated streptozotocin (STZ)-induced rats. Forty-eight weeks after STZ injection, transplanted DPSCs into unilateral hindlimb skeletal muscles. Four DPSC (i.e., 52 injection) were assessed. STZ-induced rats showed significant reductions sciatic...
Recent studies advocate that omega-3 polyunsaturated fatty acids (ω-3 PUFAs) have direct anti-oxidative and anti-inflammatory effects in the vasculature; however, role of ω-3 PUFAs Schwann cells remains undetermined.Immortalized mouse (IMS32) were incubated with docosahexaenoic acid (DHA) eicosapentaenoic (EPA). The messenger ribonucleic levels several anti-oxidant enzymes (heme oxygenase-1 [Ho-1], nicotinamide adenine dinucleotide [phosphate] H quinone oxidoreductase 1, catalase, superoxide...
Abstract Pyruvate functions as a key molecule in energy production and an antioxidant. The efficacy of pyruvate supplementation diabetic retinopathy nephropathy has been shown animal models; however, its significance the functional maintenance neurons Schwann cells under conditions remains unknown. We observed rapid extensive cell death high-glucose (> 10 mM) pyruvate-starved conditions. Exposure to these led significant decrease glycolytic flux, mitochondrial respiration ATP production,...
Pyruvate serves as a key metabolite in energy production and an anti-oxidant. In our previous study, exogenous pyruvate starvation under high-glucose conditions induced IMS32 Schwann cell death because of the reduced glycolysis–tricarboxylic acid (TCA) cycle flux adenosine triphosphate (ATP) production. Thus, this study focused on poly-(ADP-ribose) polymerase (PARP) to investigate detailed molecular mechanism death. Rucaparib, PARP inhibitor, protected cells against decreased glycolysis but...
Dental pulp stem cells (DPSCs) can be easily obtained from teeth for general orthodontic reasons. We have previously reported the therapeutic effects of DPSC transplantation diabetic polyneuropathy. As abundant secretomes DPSCs are considered to play a central role in improvement polyneuropathy, we investigated whether direct injection DPSC-conditioned media (DPSC-CM) into hindlimb skeletal muscles ameliorates polyneuropathy rats.DPSCs were isolated dental Sprague-Dawley rats. Eight weeks...
It is suggested that activation of receptor for advanced glycation end products (RAGE) induces proinflammatory response in diabetic nerve tissues. Macrophage infiltration invoked the pathogenesis polyneuropathy (DPN), while association between macrophage and RAGE downstream effects macrophages remain to be fully clarified DPN. This study explored role DPN through modified macrophages. Infiltrating impaired insulin sensitivity, atrophied neurons dorsal root ganglion, slowed retrograde axonal...
Abstract We investigated the polyol pathway activity and gene expression profiles in immortalized adult mouse Schwann cells (IMS32) under normal (5.6 m ) high (30 56 glucose conditions for 7–14 days culture. Messenger RNA protein of aldose reductase (AR) intracellular sorbitol fructose contents were up‐regulated IMS32 compared with conditions. By employing DNA microarray subsequent RT–PCR/northern blot analyses, we observed significant up‐regulation mRNA expressions serum amyloid A3 (SAA3),...