A5067632638- Hedgehog Signaling Pathway Studies
- Sphingolipid Metabolism and Signaling
- Biomedical Research and Pathophysiology
- Natural Compounds in Disease Treatment
- Genetic and Kidney Cyst Diseases
- Cancer, Lipids, and Metabolism
- Protease and Inhibitor Mechanisms
- Neonatal Health and Biochemistry
- Peptidase Inhibition and Analysis
- Autophagy in Disease and Therapy
- Kruppel-like factors research
- Drug Transport and Resistance Mechanisms
- Hemoglobin structure and function
- Porphyrin Metabolism and Disorders
- Immune Cell Function and Interaction
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Acute Lymphoblastic Leukemia research
- Caveolin-1 and cellular processes
- Cancer Research and Treatments
- Plant-derived Lignans Synthesis and Bioactivity
- Tannin, Tannase and Anticancer Activities
- Photodynamic Therapy Research Studies
- Atherosclerosis and Cardiovascular Diseases
- Axon Guidance and Neuronal Signaling
Medical University of South Carolina
2011-2025
MUSC Hollings Cancer Center
2012-2024
Istanbul Medipol University
2020-2024
Ludwig-Maximilians-Universität München
2019
Üsküdar University
2016-2019
Abdullah Gül University
2016
Fatih University
2010-2013
McGill University
2012
Inonu University
2003-2006
Abstract Mechanisms that alter protein phosphatase 2A (PP2A)‐dependent lung tumour suppression via the I2PP2A/SET oncoprotein are unknown. We show here suppressor ceramide binds selectively in nucleus and including its K209 Y122 residues as determined by molecular modelling/simulations site‐directed mutagenesis. Because was found overexpressed, whereas downregulated tumours, a sphingolipid analogue drug, FTY720, identified to mimick for binding targeting I2PP2A/SET, leading PP2A...
Ceramide forms complexes with Smad7 to inhibit cell migration–associated TGF-β–sonic hedgehog signaling in primary cilia.
Abstract Histone deacetylases (HDACs) and microRNAs (miRs) have pro‐survival roles, but the mechanism behind this is unclear. Repression of ceramide synthase 1 ( CerS1 ), altering C 18 ‐ceramide generation, was linked to drug resistance metastasis. Here we report that promoter repressed by HDAC1‐dependent inhibition Sp1 recruitment two specific GC‐boxes spanning −177 −139 region. Moreover, an alternatively spliced variant mRNA CerS1‐2 ) detected mainly in cancer cells or primary tumour...
Allogeneic hematopoietic cell transplantation (allo-HCT) is an effective immunotherapy for a variety of hematologic malignances, yet its efficacy impeded by the development graft-versus-host disease (GVHD). GVHD characterized activation, expansion, cytokine production, and migration alloreactive donor T cells. Hence, strategies to limit are highly desirable. Ceramides known contribute inflammation autoimmunity. However, their involvement in T-cell responses alloantigens undefined. In current...
Abstract Immunotherapy has transformed cancer treatment, yet durable responses to immune checkpoint blockade (ICB) are limited a subset of patients. Identifying tumor-intrinsic resistance mechanisms is critical improving ICB efficacy and outcomes. We recently identified crosstalk between lipid metabolism via the downregulation ceramide synthase 4 (CerS4) internalized programmed death-ligand 1 (PD-L1) signaling, which promotes metastasis impairs response. Our data also demonstrated that...
Abstract Sonic hedgehog (SHh) signaling is important in the pathogenesis of various human cancers, such as medulloblastomas, and it has been identified a valid target for anticancer therapeutics. The SHh inhibitor cyclopamine induces apoptosis. bioactive sphingolipid ceramide mediates cell death response to chemotherapeutic agents; however, ceramide's roles/mechanisms cyclopamine-induced apoptosis are unknown. Here, we report that generation selectively via induction neutral sphingomyelin...
Oxidative stress is linked to increased risk of gastric cancer and matrix metalloproteinases (MMPs) are important in the invasion metastasis cancer. We aimed analyze effect accumulation oxidative MKN-45 23132/87 cells following hydrogen peroxide (H2O2) exposure on expression patterns MMP-1, MMP-3, MMP-7, MMP-9, MMP-10, MMP-11, MMP-12, MMP-14, MMP-15, MMP-17, MMP-23, MMP-28, β-catenin genes.The mRNA transcripts were determined by RT-PCR. Following H2O2 exposure, viable was analyzed...
The production of L-asparaginase, an enzyme widely used in cancer chemotherapy, is mainly regulated by carbon catabolite repression and oxygen. This study was carried out to understand how different sources Vitreoscilla hemoglobin (VHb) affect the this Pseudomonas aeruginosa its VHb-expressing recombinant strain (PaJC). Both strains grown with various showed a distinct profile activity. Compared no carbohydrate supplemented medium, glucose caused slight L-asparaginase P. aeruginosa, while it...
Abnormal Wnt signaling is often observed in human cancers. Wnt5a a representative ligand that can activate both β-catenin-dependent canonical and β-catenin-independent noncanonical pathways. However, the role of carcinogenesis controversial. This study was designed to understand whether pathway its key downstream molecules such as MMP-7 β-catenin are involved gastric cancers.We analyzed expressions Wnt5a, genes 40 primary normal tumor biopsies by RT-PCR subcellular localization...
Reactive oxygen species are involved in tumor progression but how they function is not well understood. In this study, we investigated and compared the effects of hydrogen peroxide on survival, apoptosis, accumulation oxidative stress, matrix metalloprotein-7 gene expression human gastric carcinoma MKN-45 23132/87 cells.The cell lines were exposed to a dose- time-dependent manner. The surviving cells detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. stress was...
Bioactive sphingolipid ceramide is a second messenger in cell membrane response to inflammation and stress. Recent studies indicate that species with different fatty‐acid chain lengths play diverse biological functions various cellular processes, highlighting the importance of synthases (CerS) these processes. Migration mobility, part also are effected by metabolism. However, molecular mechanism CerS/ceramide involved unknown. Here, we investigated effect CerS/and on migration its related...
Abstract Programmed death-ligand 1, PD-L1 (CD274), is an immune checkpoint protein upregulated by cancer cells to facilitate evasion. also performs oncogenic functions within cells, but the underlying mechanisms of internalization and signaling remain largely unknown. Here, we report a new mechanism whereby altered ceramide metabolism via synthase 4 (CerS4) drives potentiate TGFβ/Shh/β-catenin axis drive triple-negative breast (TNBC) migration metastasis. In response metabolism, observed...
Programmed death ligand 1, PD-L1 (CD274), facilitates immune evasion and exerts pro-survival functions in cancer cells. Here, we report a mechanism whereby internalization of response to alterations bioactive lipid/ceramide metabolism by ceramide synthase 4 (CerS4) induces sonic hedgehog (Shh) transforming growth factor β receptor signaling enhance tumor metastasis triple-negative breast cancers (TNBCs), exhibiting immunotherapy resistance. Mechanistically, data showed that internalized...
Following the rapid spread and evolution of novel Corona virus starting in December 2019, lack a vaccine or medication that proved to be effective for Covid-19 was addressed as major concern by World Health Organization (WHO), Center Disease Control Prevention (CDC), U.S. Food Drug Administration (FDA) [1]. Accordingly, physicians from countries like China Korea rushed provide some potential treatment their experience treating patients Coronavirus - they used antiviral medications lopinavir,...