A5004282818- Platelet Disorders and Treatments
- Inflammatory Myopathies and Dermatomyositis
- Systemic Sclerosis and Related Diseases
- Systemic Lupus Erythematosus Research
- Blood groups and transfusion
- Eosinophilic Disorders and Syndromes
- Autoimmune Bullous Skin Diseases
- Angiogenesis and VEGF in Cancer
- Nutrition and Health in Aging
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Dysphagia Assessment and Management
- Helicobacter pylori-related gastroenterology studies
- Lymphatic System and Diseases
- Immunodeficiency and Autoimmune Disorders
- Connective Tissue Growth Factor Research
- Immune Cell Function and Interaction
- Neuroscience and Neural Engineering
- Mesenchymal stem cell research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Child Nutrition and Feeding Issues
- Trauma and Emergency Care Studies
- Photoreceptor and optogenetics research
- Genomics and Rare Diseases
National Center For Child Health and Development
2018-2025
Kochi Prefectural Education Center
2025
Nippon Medical School
2015-2024
Okayama Prefectural University
2022
University of Tsukuba
2014-2022
National Disaster Medical Center
2022
National Hospital Organization
2022
Community Link
2020
Saiseikai Central Hospital
2020
Tokyo Medical and Dental University
2020
Abstract Circulating CD14+ monocytes are precursors of phagocytes, such as macrophages and dendritic cells. Here we report primitive cells with a fibroblast-like morphology derived from human peripheral blood that can differentiate into several distinct mesenchymal cell lineages. We named this population monocyte-derived progenitor (MOMP). MOMPs were obtained in vitro mononuclear cultured on fibronectin the presence fetal bovine serum alone source growth factors. had unique molecular...
Abstract We have recently reported that in patients with chronic immune thrombocytopenic purpura (IMTP), circulating T and B cells are responsive to gpIIb-IIIa can induce anti-platelet autoantibody production. In this study, the frequencies activation status of gpIIb-IIIa-reactive were evaluated peripheral blood spleen obtained from nine IMTP undergoing splenectomy. There was no difference cell between (6.4 ± 2.6 vs 5.2 2.4 per 105 cells), as determined by limiting dilution analysis, but...
Immune thrombocytopenia purpura (ITP) is a bleeding disorder in which platelet-specific autoantibodies cause loss of platelets. In subset patients with ITP and infected Helicobacter pylori, the number platelets recovers after eradication H. pylori. To examine role pylori infection pathogenesis ITP, response 34 to treatment standard regimen, irrespective whether they were was evaluated. Eradication achieved all pylori–positive patients, significant increase observed 61% these patients. By...
We previously reported a unique CD14(+)CD45(+)CD34(+) type I collagen(+) cell fraction derived from human circulating CD14(+) monocytes, named monocyte-derived multipotential cells (MOMCs). This primitive population contains progenitors capable of differentiating along the mesenchymal and neuronal lineages. Here, we investigated whether MOMCs can also differentiate endothelial lineage. treated with angiogenic growth factors for 7 days changed morphologically adopted caudate appearance...
To evaluate upstream and downstream regulators leading to macrophage activation subsequent cytokine storm in patients with anti-melanoma differentiation-associated gene 5 (MDA5) antibody-associated interstitial lung disease (ILD).We conducted an integrated miRNA-mRNA association analysis using circulating monocytes from 3 anti-MDA5-associated ILD healthy controls identified pathways a regulator effect network by Ingenuity Pathway Analysis (IPA). The expression of relevant genes proteins was...
Thrombocytopenia is a common manifestation in patients with liver cirrhosis (LC), but its underlying mechanism remains controversial. This study examined the role of anti–platelet autoimmunity cirrhotic thrombocytopenia by determining autoantibody response to GPIIb–IIIa, major platelet surface autoantigen recognized antibodies idiopathic thrombocytopenic purpura (ITP). Circulating B cells producing anti–GPIIb–IIIa as well platelet–associated and plasma were 72 LC, 62 ITP, 52 healthy...
Abstract Objective To evaluate whether atorvastatin can increase bone marrow–derived circulating endothelial precursors (CEPs) and improve the vascular symptoms in patients with systemic sclerosis (SSc; scleroderma). Methods The study was designed as an open‐label, prospective involving 14 SSc who received 10 mg/day of for 12 weeks were followed up subsequent 4 weeks. CEPs quantified at 0 (pretreatment), 4, 8, (during treatment), 16 (posttreatment) by cell sorting 3‐color flow cytometry....
To determine whether autoantibodies to two platelet-specific antigens, glycoprotein IIb/IIIa (GPIIb/IIIa) and thrombopoietin receptor (TPOR), contribute thrombocytopenia in patients with systemic lupus erythematosus (SLE).Circulating B cells producing anti-GPIIb/IIIa antibodies serum anti-TPOR were measured 32 SLE thrombocytopenia, 30 without 92 idiopathic 60 healthy controls. The megakaryocyte density bone-marrow smears from all the was evaluated.Anti-GPIIb/IIIa antibody responses more...
To clarify the role of pentraxin 3 (PTX3), a multifunctional pattern recognition protein that can suppress fibroblast growth factor 2 (FGF-2), in systemic sclerosis (SSc)-related vasculopathy.We assessed 171 SSc patients and 19 age- sex-matched healthy control subjects. Circulating PTX3 FGF-2 levels were measured by enzyme immunoassay, CD34+CD133+CD309+ endothelial progenitor cells (EPCs) counted flow cytometry. Correlations between presence or future development vascular manifestations,...
Thrombocytopenia is a common manifestation of liver cirrhosis (LC), but its underlying mechanism not fully understood. The purpose the present paper was to evaluate platelet kinetics in LC patients by examining several non-invasive convenient markers.Fifty-seven patients, 32 with idiopathic thrombocytopenic purpura (ITP), 12 aplastic anemia (AA), and 29 healthy individuals were studied. Plasma thrombopoietin measured enzyme-linked immunosorbent assay. Absolute reticulated (RP) count plasma...
Abstract Introduction Behçet's disease (BD) is a multisystem inflammatory disorder, in which T-helper 1 (Th1)-polarized immune response plays major role the pathogenic process. We evaluated regulatory of natural killer (NK) cells Th1-biased responses patients with BD. Methods studied 47 BD, including 10 active (aBD) and 37 inactive (iBD), 29 healthy controls. The activation status cytotoxic activity NK were examined by flow cytometry. levels mRNAs for modulatory molecules determined...
ABSTRACT Lythrum anceps (Koehne) Makino (Japanese common name: “Misohagi”) is an edible plant belonging to the Lythraceae family. It mainly distributed in Asia, Northern Africa, and Europe. Plants of genus exhibit a broad range biological activities including anti‐inflammatory antimicrobial activities. Because this, plants are used traditional medicine treat hemorrhage, infected wounds, dysentery. The activation peroxisome proliferator‐activated receptor‐gamma (PPARγ) effective target for...
ABSTRACT Background Whole exome sequencing (WES) aids in diagnosing monogenic diseases, yet > 50% of all cases remain undiagnosed. We aimed to improve diagnostic precision by developing an effective WES‐based strategy for detecting congenital anomalies. Methods Initially, 128 probands with anomalies were assessed using trio‐WES and copy number variation analysis—variant interpretation was exons splice sites. Thereafter, we reanalyzed the sequence data undiagnosed following methods. First,...
Purpose: Fetal cells in maternal blood are a pure source of fetal genomic DNA for noninvasive prenatal genetic testing (NIPT), if successfully isolated. We assessed whether single-cell genome and transcriptome sequencing (G&T-seq), can isolate nucleated red (fNRBCs) suitable testing. Methods: Using umbilical cord as model, we isolated 165 single NRBC candidate from four samples, 12 lymphocytes controls one sample. G&T-seq was used to estimate the maturation stage each data integrity...
Tumor necrosis factor receptor-associated 3 (TRAF3) is an anti-inflammatory molecule that negatively regulates the non-canonical nuclear factor-κB pathway. Although TRAF3 haploinsufficiency (TRAF3 HI) can influence innate and adaptive immune cells, its effect on inflammatory bowel disease (IBD) development remains unclear. Here, we report first case of severe early-onset IBD with a novel variant leading to HI, successfully treated ustekinumab. A 6-year-old girl recurrent parotitis, otitis...
Abstract Objective To examine the prevalence, clinical associations, and pathogenic role of autoantibodies to c‐Mpl, thrombopoietin (TPO) receptor, in patients with systemic lupus erythematosus (SLE). Methods Sera from 69 SLE patients, 84 idiopathic thrombocytopenic purpura (ITP), 60 healthy individuals were screened for anti–c‐Mpl antibodies by enzyme‐linked immunosorbent assay using recombinant c‐Mpl as an antigen. Clinical findings, autoantibody profiles, serum TPO levels compared between...
Abstract Objective To detect and characterize the autoreactive CD8+ T cells to major histocompatibility complex class I chain–related gene A (MICA), a stress‐inducible antigen preferentially expressed on epithelium endothelium, in patients with Behçet's disease (BD). Methods candidate for antigenic MICA peptide was selected based its predicted binding affinity HLA–B51 proteasomal cleavage sites. Peripheral blood from 14 BD 15 healthy controls were repeatedly stimulated peptide, specific cell...
Abstract Introduction Altered phenotypes of circulating monocytes patients with systemic sclerosis (SSc) have been reported, but the role these alterations in pathogenesis SSc remains unclear. This study was undertaken to identify molecules that are preferentially expressed by monocytes, and investigate roles pathogenic process SSc. Methods We analyzed CD14 + isolated from 36 32 healthy control subjects. The monocytes' gene expression profiles were assessed Oligo GEArray ® (SABiosciences,...