A5066879089- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Phytochemicals and Antioxidant Activities
- Algal biology and biofuel production
- Mycorrhizal Fungi and Plant Interactions
- Essential Oils and Antimicrobial Activity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Botanical Research and Applications
- Sirtuins and Resveratrol in Medicine
- Fungal Biology and Applications
- Plant Stress Responses and Tolerance
- Marine and coastal ecosystems
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Postharvest Quality and Shelf Life Management
- Plant Disease Management Techniques
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Genomics, phytochemicals, and oxidative stress
- Food composition and properties
- Plant-Microbe Interactions and Immunity
- Nuts composition and effects
Center of Biotechnogy of Borj Cédria
2007-2024
Washington State University
2014-2021
Centre National de la Recherche Scientifique
2009-2010
Université de Bordeaux
2010
Chimie et Biologie des Membranes et des Nanoobjects
2009
Research and Technology Center of Energy
2008
Summary Drastic alteration in macronutrients causes large changes gene expression the photosynthetic unicellular alga Chlamydomonas reinhardtii . Preliminary data suggested that cells follow a biphasic response to this change hinging on initiation of lipid accumulation, and we hypothesized drastic repatterning metabolism also followed modality. To test hypothesis, transcriptomic, proteomic, metabolite occur under nitrogen (N) deprivation were analyzed. Eight sampling times selected covering...
Microalgae-based biofuels are promising sources of alternative energy, but improvements throughout the production process required to establish them as economically feasible. One most influential would be a significant increase in lipid yields, which could achieved by altering regulation biosynthesis and accumulation. Chlamydomonas reinhardtii accumulates oil (triacylglycerols, TAG) response nitrogen (N) deprivation. Although few important regulatory genes have been identified that involved...
Abstract The accumulation of carbon storage compounds by many unicellular algae after nutrient deprivation occurs despite declines in their photosynthetic apparatus. To understand the regulation and roles photosynthesis during this potentially bioenergetically valuable process, we analyzed structure function nitrogen model alga Chlamydomonas reinhardtii. Transcriptomic, proteomic, metabolite, lipid profiling microscopic time course data were combined with multiple measures function. Levels...
To dissect differences in both lipid accumulation and composition the role of these modifications during drought stress, four grapevine cultivars exhibiting differential tolerance to were subjected water shortage. Tolerant cultivars, Kahli Kerkennah Cardinal, exhibited higher leaf potential (Ψw), lower peroxidation compared sensitive Guelb Sardouk Superior Seedless stress. Total amounts increased stress only leaves tolerant cultivars. Drought induced increases ratios...
Recent microbiome research has shown that soil fertility, plant-associated and crop production can be affected by abiotic environmental parameters. The effect of aridity gradient on rhizosphere-soil (rhizosphere) endosphere-root (endosphere) prokaryotic structure diversity associated with Cacti remain poorly investigated understood. In the current study, next-generation sequencing approaches were used to characterize composition bacteria archaea rhizosphere endosphere Opuntia ficus-indica...
Date palm cultivation has thrived in the Gulf Cooperation Council region since ancient times, where it represents a vital sector agricultural and socio-economic development. However, climate change conditions prevailing for decades this area, next to rarefication of rain, hot temperatures, intense evapotranspiration, rise sea level, salinization groundwater, intensification cultivation, contributed increase salinity soil as well irrigation water seriously threaten date sustainability. There...
Together with leucoanthocyanidin reductase, anthocyanidin reductase (ANR) is one of the two enzymes flavonoid-biosynthesis pathway that produces flavan-3-ol monomers required for formation proanthocyanidins or condensed tannins. It has been shown to catalyse double reduction anthocyanidins form 2R,3R-flavan-3-ols, which can be further transformed 2S,3R isomers by non-enzymatic epimerization. ANR from grape (Vitis vinifera) was expressed in Escherichia coli and purified. Unexpectedly,...
Abstract Anthocyanidin reductase (ANR) from Vitis vinifera catalyzes an NADPH-dependent double reduction of anthocyanidins producing a mixture (2S,3R)- and (2S,3S)-flavan-3-ols. At pH 7.5 30°C, the first hydride transfer to anthocyanidin is irreversible, no intermediate released during catalysis. ANR reverse activity was assessed in presence excess NADP + . Analysis products by phase chiral HPLC demonstrates that acts as flavan-3-ol C 3 -epimerase under such conditions, but this only...
Nutrient deprivation causes significant stress to the unicellular microalga, Chlamydomonas reinhardtii, which responds by significantly altering its metabolic program. Following N deprivation, accumulation of starch and triacylglycerols (TAGs) is altered following massive reprogramming cellular metabolism. One protein that was found change dramatically early this TAB2, a photosystem I (PSI) translation initiation factor, whose transcript levels increased after only 30 min deprivation. A...
Most of elite cultivated grapevine varieties (Vitis vinifera L.), conventionally grafted on rootstocks, are becoming more and affected by climate changes, such as increase salinity. Therefore, we revisited the valuable genetic resources wild grapevines (V. sylvestris) to elaborate strategies for a sustainable viticulture. Here, compared physiological biochemical responses two salt-tolerant species: genotype "Tebaba" from our previous studies conventional rootstock "1103 Paulsen"....
Soil salinity is progressively impacting agriculture, including viticulture. Identification of genetic factors rendering grapevine (Vitis vinifera L.) resilience that can be introgressed into commercial varieties necessary for safeguarding viticulture against the consequences global climate change. To gain insight physiological and metabolic responses enabling salt tolerance, we compared a salt-tolerant accession Vitis sylvestris from Tunisia, "Tebaba", with "1103 Paulsen" rootstock widely...