A5032365993- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Electrocatalysts for Energy Conversion
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Advanced battery technologies research
- Carbon and Quantum Dots Applications
- Laser-Ablation Synthesis of Nanoparticles
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
- Graphene and Nanomaterials Applications
- Advanced Photocatalysis Techniques
- RNA modifications and cancer
- Electrochemical Analysis and Applications
- Supercapacitor Materials and Fabrication
- Catalytic Processes in Materials Science
- Conducting polymers and applications
- Advanced Sensor and Energy Harvesting Materials
- PARP inhibition in cancer therapy
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
Institute for Basic Science
2017-2024
Korea Institute of Industrial Technology
2019-2023
Hanyang University
2019-2020
Ulsan National Institute of Science and Technology
2018-2019
Government of the Republic of Korea
2019
Howard Hughes Medical Institute
2010-2017
Massachusetts Institute of Technology
2010-2017
IIT@MIT
2013
Seoul National University
1998-2011
UNC Lineberger Comprehensive Cancer Center
1999
The origin recognition complex (ORC) specifies replication location. Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence binding. Using high-throughput sequencing to map binding and nucleosome positioning, we show that yeast origins characterized by an asymmetric pattern positioned nucleosomes flanking ACS. sequences sufficient...
The ex-situ incorporation of the secondary SiC reinforcement, along with in-situ tertiary and quaternary Mg3N2 Si3N4 phases, in primary matrix Mg2Si is employed order to provide ultimate wear resistance based on laser-irradiation-induced inclusion N2 gas during laser powder bed fusion. This substantialized both thermal diffusion- chemical reaction-based metallurgy Mg2Si–SiC/nitride hybrid composite. study also proposes a functional platform for systematically modulating functionally graded...
Abstract Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC RLCs cooperate to regulate PCNA loading unloading. Here, we show distinct or unloading activity each clamp loader. ATAD5-RLC possesses potent activity. ATPase motif collar domain ATAD5 are crucial for DNA structures did not affect ATAD5-RLC. could unload ubiquitinated PCNA. Through single molecule measurements,...
Graphene oxide quantum dots (GOQDs) are usually prepared using expensive carbon precursors such as nanotubes (CNT) or graphene under the strong acidic condition, which requires an additional purifying process. Here, we first develop a facile pulsed laser ablation in liquid (PLAL) technique for preparing GOQDs earth-abundant and low-cost coal precursor. Only ethanol used to produce with excellent optical properties. The exhibit optoelectronic properties can be successfully utilized bioimaging...
The triboelectric performance of the SMC-PDMS-based TENG was improved by enhancing capacitance device owing to synergistic effect superior dispersion degree and permittivity SMCs.
With respect to the detection of Fe
The ring-shaped MCM helicase is essential to all phases of DNA replication. complex loads at replication origins as an inactive double-hexamer encircling duplex DNA. Helicase activation converts this species two active single hexamers that encircle single-stranded (ssDNA). molecular details interactions during these events are unknown. We determined the crystal structure Pyrococcus furiosus N-terminal domain hexamer bound ssDNA and define a conserved MCM-ssDNA binding motif (MSSB)....
Eukaryotic replication origin licensing, activation and timing are influenced by chromatin but a mechanistic understanding is lacking. Using reconstituted nucleosomal DNA assays, we assessed the impact of nucleosomes on initiation. To generate distinct landscapes, different chromatin-remodeling enzymes (CREs) were used to remodel origin-DNA templates. Nucleosomal organization two steps initiation: licensing helicase activation. Origin assays showed that local nucleosome positioning enhanced...
Abstract The development of earth‐abundant and efficient oxygen evolution reaction (OER) electrocatalysts is necessary for green hydrogen production. preparation OER requires both the adsorption sites charge transfer on catalyst surface to be suitably engineered. Herein, design an electrocatalyst reported with significantly enhanced water oxidation performance via dual‐phase engineering, which displays a high number facile transfer. More importantly, simple chemical etching process enables...
R-loops are formed when replicative forks collide with the transcriptional machinery and can cause genomic instability. However, it is unclear how regulated at transcription-replication conflict (TRC) sites replisome proteins to prevent R-loop formation or mediate tolerance. Here, we report that ATAD5, a PCNA unloader, plays dual functions reduce both under normal replication stress conditions. ATAD5 interacts RNA helicases such as DDX1, DDX5, DDX21 DHX9 increases abundance of these...
Quaternary multimetallic LDHs have been developed through a facile metal–organic-framework derived electrochemical activation process. Ni–Fe–Al–Co quaternary show one of the best OER performances in alkaline and seawater electrolytes.
Triboelectric nanogenerators (TENGs) as energy harvesters have been extensively investigated due to their ability convert mechanical electricity through the effective coupling of triboelectrification and electrostatic induction. Herein, we introduce polydimethylsiloxane (PDMS)-based TENG prepared using ZnSnO3 (ZTO) nanostructure on surface-modified carbon nanotubes (SMCs), which shows high power density suitable different types practical applications in harvesting self-power system. with 0.3...
To tune the electronic and optoelectronic properties of graphene quantum dots (GQDs), heteroatom doping (e.g., nitrogen (N), boron (B), sulfur (S)) is an effective method. However, it difficult to incorporate S into carbon framework GQDs because atomic size much larger than that C atoms, compared N B. In this study, we report a simple one-step method for synthesis sulfur-doped (S-GQDs) via pulsed laser ablation in liquid (PLAL) process. The as-prepared S-GQDs exhibited enhanced fluorescence...
Maintaining stability of replication forks is important for genomic integrity. However, it not clear how replisome proteins contribute to fork under stress. Here, we report that ATAD5, a PCNA unloader, plays multiple functions at stalled including promoting its restart. ATAD5 depletion increases instability upon hydroxyurea treatment in cultured cells and mice. recruits RAD51 an ATR kinase-dependent manner by hydroxyurea-enhanced protein-protein interactions timely removes from recruitment....
The design of atomically dispersed single atom catalysts (SACs) must consider high metal-atom loading amount, effective confinement, and strong interactions with matrix, which can maximize their catalytic performance. Here reported is a promising method to synthesize SACs on highly conductive multiwall carbon nanotube (MWCNT) supports using pulsed laser confinement (PLC) process in liquid. Atomic cobalt (Co) phosphorus (P) density are homogeneously incorporated the outer wall MWCNT (Co–P SAC...
A hatching-distance-controlled lattice of 65.1Co28.2Cr5.3Mo is additively manufactured via laser powder bed fusion with a couple periodic and aperiodic arrangements nodes struts. Thus, the proposed has an amorphous-inspired structure in short- long-range orders. From structural perspective, artificial intelligence algorithm used to effectively align lattices various hatching distances. Then, metastable combination exhibits unexpectedly high specific compression strength that only slightly...
Graphene quantum dots (GQDs) and graphene oxide (GOQDs) can be used in different applications such as optoelectronic biomedical applications, respectively. Hence, the selective synthesis of GQDs GOQDs is highly desirable but challenging. Here, we present selectively prepared by an easy simple pulsed laser ablation liquid (PLAL) method controlling wavelength. The obtained showed a significantly nature mainly due to existence surface oxygen-rich functional groups (e.g. carboxyl or hydroxy...