A5049234367- Genomics and Phylogenetic Studies
- Parasitic Infections and Diagnostics
- Antimicrobial Resistance in Staphylococcus
- Bacterial Infections and Vaccines
- Bacillus and Francisella bacterial research
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Antifungal resistance and susceptibility
- RNA modifications and cancer
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
Houston Methodist
2022-2025
Methodist Hospital
2022-2023
Texas Center for Infectious Disease
2022
The University of Texas Health Science Center at Houston
2022
Rice University
2021
Multiple displacement amplification (MDA) outperforms conventional PCR in long fragment and whole-genome amplification, making it attractive to couple MDA with long-read sequencing of samples limited quantities DNA obtain improved genome assemblies. Here, we explore the efficacy limits for efficient low-cost sequence assembly using Oxford Nanopore Technologies (ONTs) rapid library preparations minION sequencing. We successfully generated almost complete sequences all organisms examined,...
Patients with recalcitrant enterococcal bloodstream infections are at greater risk of adverse outcomes. We identified patients in the 2016-2022 Vancomycin-Resistant Enterococcal Bacteremia Outcomes Study (VENOUS) cohort experiencing for further clinical and genomic characterization. episodes were considered "persistent" if there was a lack clearance on day four while receiving ≥ 48 hours active therapy recurrent during hospitalization subsequent positive culture (collectively, "recalcitrant"...
P. aeruginosa is one of the most common causative agents for ventilator-associated pneumonia and nosocomial bacteremia a leading cause death in patients with cystic fibrosis. Pandrug-resistant strains are increasingly identified clinical samples show resistance to virtually all major classes antibiotics, including aminoglycosides, cephalosporins, carbapenems.
ABSTRACT Multiple Displacement Amplification (MDA) outperforms conventional PCR in long fragment and whole genome amplification which makes it attractive to couple with long-read sequencing of samples limited quantities DNA obtain improved assemblies. Here, we explore the efficacy limits MDA for sequence assembly using Oxford Nanopore Technologies (ONT) rapid library preparations minION sequencing. We successfully generated almost complete sequences all organisms examined, including...
Abstract Background Candida spp. colonization is a risk factor for candidemia in intensive care unit (ICU) patients. Recent data suggest that the epidemiology of infections shifting away from C. albicans, but prospective on gut are limited. Methods We conducted cohort study adult patients admitted to an ICU at tertiary hospital. Patients had stool samples collected twice weekly up four weeks or until discharge. A convenience sample was plated CHROMagar Plus 48 hours. Colonies different...