A5021632580- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Respiratory viral infections research
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Bacterial biofilms and quorum sensing
- Breast Implant and Reconstruction
- Bacterial Infections and Vaccines
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Probiotics and Fermented Foods
- Berberine and alkaloids research
- Reconstructive Facial Surgery Techniques
- Gut microbiota and health
- Surgical site infection prevention
- Pediatric health and respiratory diseases
- Viral Infections and Immunology Research
- Neutropenia and Cancer Infections
- Tuberculosis Research and Epidemiology
- Aquaculture disease management and microbiota
- Plant-based Medicinal Research
The University of Texas Health Science Center at Houston
2019-2024
CNR de la Résistance aux Antibiotiques
2021
Ochsner Medical Center
2016-2017
Translational Research Institute
2016
Louisiana State University
2015
Abstract Though rare, breast implant-associated anaplastic large cell lymphoma (BIA-ALCL), a CD30+ T-cell associated with textured implants, has adversely impacted our perception of the safety implants. Its etiology unknown, one hypothesis suggests an initiating inflammatory stimulus, possibly infectious, triggers BIA-ALCL. We analyzed microbiota breast, skin, implant and capsule in BIA-ALCL patients (n = 7), controls via culturing methods, 16S rRNA microbiome sequencing,...
Background. Patients with recalcitrant enterococcal bloodstream infections are at greater risk of adverse outcomes. We identified patients in the 2016-2022 Vancomycin-Resistant Enterococcal Bacteremia Outcomes Study (VENOUS) cohort experiencing for further clinical and genomic characterization. Methods. episodes were considered persistent if there was a lack clearance on day four while receiving least 48 hours active therapy recurrent during hospitalization subsequent positive culture...
Vancomycin-resistant enterococci (VRE) are major therapeutic challenges. Prospective contemporary data characterizing the clinical and molecular epidemiology of VRE bloodstream infections (BSIs) lacking.
Daptomycin (DAP) is often used as a first-line therapy to treat vancomycin-resistant
The siderophore-cephalosporin cefiderocol (FDC) presents a promising treatment option for carbapenem-resistant (CR)
Polymyxin resistance is an increasing problem worldwide. Currently, determining susceptibility to polymyxins problematic and lengthy. Polymyxins diffuse poorly into agar, potentially giving inaccurate disk diffusion Etest results. A rapid screening test (2 h) for the detection of polymyxin in Enterobacteriaceae, developed by P. Nordmann L. Poirel (rapid NP test) 2016, detects glucose metabolization presence E (PE) PB via pH-induced color change. The sensitivity specificity were 99.3 95.4%,...
The siderophore-cephalosporin cefiderocol(FDC) presents a promising treatment option for carbapenem-resistant(CR) P. aeruginosa (PA). FDC circumvents traditional porin and efflux mediated resistance by utilizing TonB-dependent receptors (TBDRs) to access the periplasmic space. Emerging has been associated with loss of function mutations within TBDR genes or regulatory controlling expression. Further, difficulties antimicrobial susceptibility testing (AST) unexpected negative clinical...
ABSTRACT Bacterial infection is the most common complication following staged post-mastectomy breast reconstruction initiated with a tissue expander (TE). To limit bacterial infection, antibiotic irrigation of surgical site commonly performed despite little high-quality data to support this practice. We prospective randomized control trial compare impact saline alone triple regimen (1 g cefazolin, 80 mg gentamicin, and 50,000 units bacitracin in 500 mL saline) for implant surgery. The...
Vibrio vulnificus, an inhabitant of marine and estuarine environments around the world, is leading cause reported seafood-related deaths in United States. Disease caused by opaque colony-forming strains that produce capsular polysaccharide, loss which results unencapsulated translucent phenotype with diminished virulence potential. Rugose a third phenotypic variant V. produces separate exopolysaccharide dry, wrinkled appearance ability to form profuse biofilms. Phase variation among these...
Abstract Background Penicillin-binding protein 4 (PBP4) is a low affinity PBP that has been associated with decreased susceptibility to penicillins in Enterococcus faecalis (Efs). In vitro data have shown changes the promoter region leading increased pbp4 gene expression and amino acid resulting active site remodeling contribute this phenotype. There limited on prevalence of these strains United States. We investigated β-lactam trends association variations PBP4 (allotypes) upstream region....
Abstract Background Non–Enterococcus faecium, non–E. faecalis (NFF) enterococci are a heterogeneous group of clinically pathogenic that include species with intrinsic low-level vancomycin resistance. Patients cancer at increased risk for bacteremia NFF enterococci, but their clinical and molecular epidemiology have not been extensively described. Methods We conducted retrospective review all patients (n = 70) from 2016 to 2022 major center. The main outcomes assessed were 30-day mortality,...
ABSTRACT Daptomycin (DAP) is often used as a first line therapy to treat vancomycin-resistant Enterococcus faecium (VR Efm ) infections but emergence of DAP non-susceptibility threatens the effectiveness this antibiotic. Moreover, current methods determine MICs have poor reproducibility and accuracy. In enterococci, resistance mediated by LiaFSR cell membrane stress response system deletion liaR encoding regulator results in hypersusceptibility antimicrobial peptides. The main genes...
Abstract Background Penicillin-binding protein 4 (PBP4) is a low affinity PBP that has been associated with decreased susceptibility to penicillins in Enterococcus faecalis (Efs). Changes the promoter region leading increased expression of pbp4 gene contribute this phenotype. There limited data on clinical outcome patients infected these strains U.S. We investigated outcomes Efs bacteremia stratified by PBP4 type and piperacillin MIC. Methods Index bloodstream isolates from 167 were selected...
In the United States, vanB-mediated resistance in enterococci is rare. We characterized three sequence type (ST) 6, vancomycin-resistant Enterococcus faecalis isolates causing bacteremia unique patients spatiotemporally distinct settings. Isolates were recovered between 2018 and 2020 two cities States (Houston, TX; Miami, FL). The harbored vanB operon on a chromosomally located Tn1549 transposon, epidemiological data suggested multiple introductions of gene cluster into ST6 E. faecalis.
Abstract Background Cefiderocol (FDC) is an iron siderophore cephalosporin with activity against multi-drug resistant (MDR) Gram-negative bacteria. Despite high rates of in vitro susceptibility, treatment failure the emergence resistance still a concern. Heteroresistance, or presence sub-population strain that tests susceptible by standard methods, has been suggested as potential mechanism for this observation. Previously, we showed MDR Pseudomonas aeruginosa (PA) changes transporters (TBDR)...
Abstract Background Cefiderocol (FDC) is a novel siderophore cephalosporin that retains activity against MDR gram-negative bacteria. In P. aeruginosa (PA), FDC utilizes TonB-dependent receptors (TBDR) PirA, PiuA, or PiuD to enter the periplasmic space. We recently reported clinical PA isolate developed elevated MICs associated with mutations in genes encoding TBDRs absence of prior exposure FDC. this study, we investigated frequency TBDR not cefiderocol among stains recovered from 1999 2018...
Abstract Background Vancomycin-resistant enterococci (VRE) are a major cause of nosocomial bloodstream infections. Enterococci exhibit remarkable genomic plasticity and can recombine through the acquisition genetic material via mobile elements (MGEs), including resistance genes. The accessory genome plays role in evolution within human host. Thus, dissecting entire (pan-genome) is paramount importance to characterize population structure causing disease. Methods VENOUS an ongoing...
Abstract Background The Biofire® FilmArray Meningitis Encephalitis (FAME) is a multiplex polymerase chain reaction (PCR) test can rapidly detect up to 14 pathogens that cause meningitis and encephalitis. impact on preventing repeat lumbar punctures obtain more diagnostic studies currently unknown. Methods Patients admitted Memorial Hermann Hospital (MHH) between July 2018-February 2019 with community-acquired symptoms of or encephalitis, CSF white blood cell count >5 cells/mm3, leftover...
Abstract Background E. faecalis (Efc) isolates are usually susceptible to ampicillin (AMP). AMP-based regimens the standard of care for enterococcal infections, although other antibiotics often used as definitive treatment. We thus compared outcomes patients with cancer and Efc bacteremia treated AMP-containing (ACR) non-AMP-containing antibiotic (NACR). Methods A multicenter, prospective, observational cohort study conducted at MD Anderson Cancer Center, Henry Ford Hospital, Memorial...
Abstract Background The majority of adults and children with meningitis encephalitis have unknown etiologies fostering universal admission, prolonged length stay, empirical antibiotic antiviral therapies. A qualitative multiplexed nucleic acid-based in vitro diagnostic test (FilmArray) is helping clinicians identify organisms improve clinical outcomes. Methods Patients presenting between July 5, 2018, to April 26, 2021, or encephalitis, cerebrospinal fluid (CSF) WBC >5 cells/mm3,...