A5069428984- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Veterinary Equine Medical Research
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Antifungal resistance and susceptibility
- Thyroid Cancer Diagnosis and Treatment
- Microbial Metabolism and Applications
- Ruminant Nutrition and Digestive Physiology
- Mycobacterium research and diagnosis
- Meat and Animal Product Quality
- Biosensors and Analytical Detection
- Reproductive Physiology in Livestock
- Bacterial biofilms and quorum sensing
- Biochemical and Structural Characterization
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 detection and testing
- Cancer survivorship and care
- BRCA gene mutations in cancer
- Aquaculture disease management and microbiota
- S100 Proteins and Annexins
- Insect and Pesticide Research
- Renal and Vascular Pathologies
- Enterobacteriaceae and Cronobacter Research
Millennium Initiative for Collaborative Research on Bacterial Resistance
2019-2025
Clínica Alemana
2019-2025
Universidad del Desarrollo
2019-2025
University of Castilla-La Mancha
2023
Universidad del Desarrollo del Estado de Puebla
2023
International Flavors & Fragrances (France)
2023
University of La Salle
2022
Universidad de Antioquia
1989-2021
Millennium Science Initiative
2019-2021
Children's Mercy Hospital
2019
The impact of coronavirus disease 2019 (COVID-19) on antimicrobial use (AU) and resistance has not been well evaluated in South America. These data are critical to inform national policies clinical care.
Abstract Background The global spread of methicillin-resistant Staphylococcus aureus (MRSA) is associated with distinct clones predominating in specific geographical regions. Available evidence suggests that the Chilean-Cordobes clone (ChC), an ST5-SCCmecI lineage, has predominated Chilean hospitals ever since its first description 1998. However, we recently reported a gradual decrease this lineage over time, frequencies dropping from 94% (2000) to 52% (2016). To understand clonal...
Abstract Background Accurate estimates of gut colonization with drug-resistant gram-negative bacilli (GNB) are key to combat antimicrobial resistance. Data on best practices assess is scarce, limiting the comparability and feasibility screening efforts. Building upon ongoing projects studying carbapenem-resistant (CR), carbapenemase-producing (CP-CR) extended-spectrum beta-lactamase-producing (ESBL) GNB in Chile, we compared 3 different strategies assessed impact time between swab collection...
Abstract Background Penicillin (PEN) and ampicillin (AMP) resistance is rare in E. faecalis, may arise via increased expression of penicillin-binding protein 4 (PBP4), mutations affecting PBP4’s affinity to β-lactams, rarely β-lactamase production. We recently identified 23 vanA vancomycin-resistant faecalis (VREfs), recovered from 19 patients hospitals Chile (2020-2023) that exhibited PEN unusually elevated AMP MICs (4-16 µg/mL). Here, we characterize the molecular basis reduced β-lactam...
ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is a major public health menace. The global spread of MRSA characterized by successive waves epidemic clones dominating specific geographical regions. acquisition genes encoding resistance to heavy metals (HMRGs) thought be key feature in the geographic divergence MRSA. However, cause-effect relationship between presence HMRGs and remains clarified. In this study, we assessed role that may have played evolutionary ST5-SCC mec I...
In most of the world, diagnostic surgery remains frequent approach for indeterminate thyroid cytology. Although several molecular tests are available testing in centralized commercial laboratories United States, there no kits local laboratory testing. The aim this study was to develop a prototype vitro (IVD) gene classifier further characterization nodules with an cytology.In first stage, expression 18 genes determined by quantitative polymerase chain reaction (qPCR) broad histopathological...
The siderophore-cephalosporin cefiderocol (FDC) presents a promising treatment option for carbapenem-resistant (CR)
The global dissemination of methicillin-resistant Staphylococcus aureus (MRSA) is associated with the emergence and establishment clones in specific geographic areas. Chilean-Cordobes clone (ChC) (ST5-SCC
// Soledad Urra 1 , Martin C. Fischer José R. Martínez Loreto Véliz 2 Paulina Orellana Antonieta Solar 3 Karen Bohmwald 4 Alexis Kalergis 4, 5 Claudia Riedel 6 Alejandro H. Corvalán 7 Juan Roa Rodrigo Fuentealba 8 Joaquin Cáceres 9 Marcelo López-Lastra Augusto León Nicolás Droppelmann and Hernán E. González 1, Department of Surgical Oncology, Faculty Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile Physiology, Biological Sciences, Pathology, Millennium Institute on...
The siderophore-cephalosporin cefiderocol(FDC) presents a promising treatment option for carbapenem-resistant(CR) P. aeruginosa (PA). FDC circumvents traditional porin and efflux mediated resistance by utilizing TonB-dependent receptors (TBDRs) to access the periplasmic space. Emerging has been associated with loss of function mutations within TBDR genes or regulatory controlling expression. Further, difficulties antimicrobial susceptibility testing (AST) unexpected negative clinical...
Background:Cortisol is indicative of adaptation to physiological factors, disease and environmental stress in equines.Cortisol recently gained attention for its prognostic value critical as an indicator fi tness athletic performance.Objective: To determine baseline serum cortisol (SC) morning concentrations the Colombian creole horse (CCH).Methods: Blood was taken from 62 CCH both genders with average age 8.8 years (± 5.3), which were clinically healthy had diff erent performance...
Ceftazidime/Avibactam (CAZ/AVI) is frequently used to treat KPC-producing Pseudomonas aeruginosa (KPC-PA) and Enterobacterales. CAZ/AVI resistance driven by several mechanisms. In P. this mainly occurs through alteration of AmpC, porins, and/or efflux pump overexpression, whereas in Enterobacterales it D179Y substitution the active site KPC enzyme. This aminoacid change abolishes AVI binding site, hence inhibition impaired. However, also decreases KPC-mediated carbapenems ("see-saw" effect)....
Background: Equine gastric ulcer syndrome (EGUS) has been associated with duration of confinement, grain supplementation, exercise, stress, use non-steroidal anti-inflammatory drugs, and the microbiome. On other hand, limited information is available on risk factors for EGUS in Colombia. Objective: To determine frequency presentation several municipalities Antioquia. Methods: A total 103 male female horses were evaluated. Endoscopic samples from different portions stomach taken subjected to...
Identify molecular mechanisms responsible for the in vitro non-susceptibility to ceftolozane/tazobactam (TOL) a group of 158 clinical isolates Pseudomonas aeruginosa from five Latin American countries collected before introduction TOL into practice.Clinical P. (n = 504) were between January 2016 and October 2017 20 hospitals located Argentina, Brazil, Chile, Colombia, Mexico. Minimum inhibitory concentrations (MICs) determined by standard broth microdilution interpreted according CLSI...
Purpose: Colistin resistance mechanisms involving mutations in chromosomal genes associated with LPS modification are not completely understood. Mutations coding for the MgrB regulator frequently account colistin Klebsiella pneumoniae , whereas PhoPQ and PmrAB frequent E. coli . Our aim was to perform a genetic analysis of colistin-resistant (MIC ≥ 4 μg/mL) clinical isolates K. (n = 8) 7) different STs. Methods: Isolates were obtained 3-year period university hospital Santiago, Chile....
Background/Objective: The use of new technologies in rehabilitation to evaluate and improve occupational performance quality life is increasing. Technological applications the health field could help meet needs patients, including those women breast cancer survivors. main aim this study was design a mobile phone application "MAIA" focused on perceived who have had achieve optimal their daily lives meaningful way. Methods: A cross-sectional usability using an online questionnaire designed....
equine gastric ulcer syndrome (EGUS) has a multifactorial nature and it affects both the squamous glandular mucosa of stomach. Multiple therapeutic strategies involving large periods medication are used to treat this condition. Objective: evaluate effects administering corn oil (CO) horses with induced ulcers, describe mechanism action mucosal repair. Methods: fifteen divided into three groups were used. A combination confinement phenylbutazone was during ulcer-induction phase. After...
Ceftaroline (CPT) is a broad-spectrum agent with potent activity against methicillin-resistant Staphylococcus aureus (MRSA). The sequence type 5 (ST5) Chilean-Cordobés clone, associated CPT nonsusceptibility, dominant in Chile, region high rates of MRSA infections. Here, we assessed the vitro collection isolates collected between 1999 and 2018 from nine hospitals (n = 320) community settings 41) Santiago, evaluated performance across testing methodologies. We found that our...
Ceftolozane/tazbactam (C/T) is a potent anti-pseudomonal agent that has clinical utility against infections caused by non-carbapenemase, producing-carbapenem-resistant Pseudomonas aeruginosa (non-CP-CR-PA). Accurate, precise, and reliable antimicrobial susceptibility testing (AST) crucial to guide decisions. However, studies assessing the performance of different AST methods non-CP-CR-PA (the main niche for C/T), are lacking. Here, we evaluated gradient strips (Etest MIC test strip [MTS],...
Abstract Background The MRSA Chilean-Cordobes (ChC) clone belongs to the clonal complex 5 (CC5) and typically carries SCCmec I. ChC predominated widely throughout several countries of Latin America (LA), but during mid-2000s a CA-MRSA CC8 LA variant (USA300-LV) quickly replaced in Colombia Ecuador. Most notably, this replacement was not observed Peru or Chile. Here, we aimed understand phylogenomic relatedness CC5 obtained from different LA. Methods We sequenced analyzed genomes 115 isolates...
Carbapenem-resistant Pseudomonas aeruginosa (CRPA) is one of the pathogens that urgently needs new drugs and alternatives for its control. The primary strategy to combat this bacterium combining treatments beta-lactam with a beta-lactamase inhibitor. most used combinations against P. are ceftazidime/avibactam (CZA) ceftolozane/tazobactam (C/T). Although mechanisms leading CZA C/T resistance have already been described, among which resistance-nodulation-division (RND) efflux pumps, role these...
Carbapenemase-producing carbapenem-resistant Enterobacterales (CP-CRE) represent a significant global threat. The emergence of dual CP-CRE is particularly alarming, as they can potentially compromise the efficacy newer antibiotics, further decreasing therapeutic alternatives. Herein, we report multiple species recovered from invasive infections in Chile that simultaneously harbor blaKPC and blaNDM provide an in-depth genomic characterization these worrisome pathogens. We collected (CRE)...