A5040366438- SARS-CoV-2 detection and testing
- Antifungal resistance and susceptibility
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Plant Pathogens and Fungal Diseases
- Fungal Infections and Studies
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 and COVID-19 Research
- Bacterial Identification and Susceptibility Testing
- Viral gastroenteritis research and epidemiology
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Infectious Diseases and Mycology
- Respiratory viral infections research
- Nursing care and research
- Enterobacteriaceae and Cronobacter Research
- Diphtheria, Corynebacterium, and Tetanus
- Amoebic Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Food Safety and Hygiene
- Medical research and treatments
- Otolaryngology and Infectious Diseases
- Antibiotic Use and Resistance
- Child Nutrition and Water Access
Clínica Alemana
2020-2024
Universidad del Desarrollo
2020-2024
Pontificia Universidad Católica de Chile
2012-2023
Universidad del Desarrollo del Estado de Puebla
2020
Hospital Clínico de la Universidad Católica
2020
University of Memphis
2018
University of London
2011-2016
London School of Hygiene & Tropical Medicine
2011-2016
Abstract In the context of Covid-19 pandemic, development and validation rapid easy-to-perform diagnostic methods are high priority. We compared performance four antigen detection tests for SARS-CoV-2 in respiratory samples. Immunochromatographic assays from RapiGEN, Liming bio, Savant, Bioeasy were evaluated using universal transport medium containing naso-oropharyngeal swabs suspected cases. The accuracy was determined comparison to RT-PCR. A total 111 samples included; 80 RT-PCR positive....
Background Active surveillance is necessary for improving the management and outcome of patients with candidemia. The aim this study was to describe epidemiologic clinical features candidemia in children adults tertiary level hospitals Chile. Methods We conducted a prospective, multicenter, laboratory-based survey 26 care Chile, from January 2013 October 2017. Results A total 780 episodes were included, median incidence 0.47/1,000 admissions. Demographic, microbiological information 384...
Real-Time Reverse-Transcription Polymerase Chain Reaction (RT-PCR) is currently the only recommended diagnostic method for SARS-CoV-2. However, rapid immunoassays SARS-CoV-2 antigen could significantly reduce COVID-19 burden weighing on laboratories around world.We evaluated performance of two fluorescence (FIAs), SOFIA SARS Antigen FIA (Quidel Corporation, San Diego, CA, USA) and STANDARD F Ag (SD Biosensor Inc., Gyeonggi-do, Republic Korea), which use an automated reader. The study used 64...
Abstract Background Healthcare workers (HCWs) are at high risk of exposure to SARS-CoV-2. Cross-sectional studies have provided variable rates seroprevalence in HCWs. Longitudinal assessments the serological response Covid-19 among HCWs crucial understanding infection and changes antibody titers over time. We aimed investigate factors associated with seroconversion a prospective cohort during peak first wave pandemic. Methods conducted longitudinal study 446 front-line HCWsin tertiary-care...
Infectious diarrhea can be caused by a large number of microorganisms including bacteria virus and parasites. The clinical syndromic approach has been traditionally used to guide therapy. aim this study was characterize the etiology acute FilmArray GI panel correlate it with its presentation in an adult population presenting emergency room developing country.Adult patients attending ER due were selected. All included had performed characteristics recorded.One hundred ninety-nine included....
Impact of mass spectrometry by MALDI-TOF MS for the rapid identification aerobic and anaerobic bacteria clinical importanceBackground: (Matrix Assisted Laser Desorption Ionization -Time Flight Mass Spectrometry) technology, recently introduced in microbiology laboratory has proven to be a precise method bacterial identification.Objective: To evaluate performance, costs associated turnaround time routine laboratory.Material Method: Five hundred sixty one isolates (281 aerobes 280 anaerobes)...
Global dissemination of mcr-like genes represents a serious threat to public health since it jeopardizes the effectiveness colistin, an antibiotic used as last-resort treatment against highly antibiotic-resistant bacteria. In 2017, mcr-1-positive isolate Escherichia coli was found in Chile for first time. Herein we report genetic features this strain (UCO-457) by whole-genome sequencing (WGS) and conjugation experiments. The UCO-457 belonged ST4204 carried 285 kb IncI2-type plasmid...
Abstract The evaluated SARS-CoV-2 antigen rapid fluorescence immunoassays reliably identified patients within the first 5 days of symptom onset, when respiratory secretions carried high viral loads. This performance suggests that these tests might play an important role for future PCR-independent strategies to detect early or infective cases.
Campylobacter spp. is an emerging cause of infectious diarrhea worldwide. In South American countries such as Chile, its prevalence underestimated due to inadequate detection methods. Gastrointestinal multiplex PCR panels (GMP) permit rapid and sensitive bacterial pathogens provide important epidemiological information. This study aimed analyze epidemiology using the results molecular methods compare those culture We performed a retrospective, descriptive analysis detected in clinical stool...
Background: Filamentous fungi are an emergent cause of severe infections in immunocompromised patients. Timely and accurate identification is crucial to initiate appropriate therapy. Traditional methods time-consuming, labor-intensive, operator-dependent. Ma-trix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry a rapid easy-to-perform method. This study evaluates the effectiveness commercial MALDI-TOF MS platform identify filamentous routine laboratory....
Background: In the context of COVID-19 pandemic, development and validation rapid easy-to-perform diagnostic methods are high priority to enable required testing scale up in most countries. We evaluated a novel antigen detection test (RDT) for SARS-CoV-2 respiratory samples.Method: sA new fluorescence immunochromatographic (Bioeasy Biotechnology Co., Shenzhen, China) was nasophayngeal (NP) oropharyngeal (OP) swabs from patients with suspected Santiago, Chile. Diagnostic accuracy determined...
Purpose: Colistin resistance mechanisms involving mutations in chromosomal genes associated with LPS modification are not completely understood. Mutations coding for the MgrB regulator frequently account colistin Klebsiella pneumoniae , whereas PhoPQ and PmrAB frequent E. coli . Our aim was to perform a genetic analysis of colistin-resistant (MIC ≥ 4 μg/mL) clinical isolates K. (n = 8) 7) different STs. Methods: Isolates were obtained 3-year period university hospital Santiago, Chile....
Invasive Candida spp. infections have been described more frequently.To characterize the epidemiological data of candidemia in recent years.A retrospective study adult patients a University Hospital Santiago, Chile, with 1 or documented episodes candidemia, from January 2000 to December 2013.One hundred and twenty were identified 120 patients, annual incidence 0.4 cases per 1000 discharges, 53.3% male 58.3% > 60 years, 77,5% had at least one co-morbidity. albicans was species most frequently...
Introducción: La enfermedad fúngica invasora (EFI) por hongos filamentosos es cada vez más frecuente.
Streptococcus pyogenes (group A Streptococcus) causes diseases ranging from mild pharyngitis to severe invasive infections. The N-terminal fragment of streptococcal M protein elicits protective antibodies and is an attractive vaccine target. However, this N- terminal hypervariable: there are more than 200 different types. In study, intranasal live bacterial comprising 10 strains Lactococcus lactis, each expressing one protein, has been developed. Live bacterial-vectored vaccines cost less...
La nocardiosis es una infección localizada o sistémica que afecta principalmente a pacientes inmunocomprometidos, siendo la localización pulmonar el sitio más frecuente. transmisión proviene de inhalación esporas mediante inoculación directa en piel y mucosa ocular. Se han descrito 90 especies Nocardia, éstas mitad son reconocidas como patógenos humanos. Las importancia médica conocidas Nocardia farcinica, abscessus, nova brasiliensis. En Chile hay casos publicados por asteroides N....
Recently it was described the plasmidial gene mcr-1 associated with colistin resistance. We screened by PCR and sequencing for in thirteen clinical isolates resistant to colistin. observed amplification one E. coli. To our knowledge, this is first report of presence Chile.
ABSTRACT Protein subunit vaccines are often preferred because of their protective efficacy and safety. Lactic acid bacteria expressing heterologous antigens constitute a promising approach to vaccine development. However, safety in terms toxicity bacterial clearance must be evaluated. Anti‐ Streptococcus pyogenes ( S. ) face additional concerns they may elicit autoimmune responses. The assessment toxicity, autoimmunity an anti‐streptococcal based on Lactococcus lactis L. 10 different M...
Introducción: La enfermedad fúngica invasora (EFI) se reconoce como causa importante de morbi-mortalidad en pacientes crÃticos. mayorÃa estas infecciones son provocadas por Candida spp. para cuyo diagnóstico existen importantes limitaciones.