A5081837050- Parasitic Infections and Diagnostics
- Toxoplasma gondii Research Studies
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- MXene and MAX Phase Materials
- Fungal and yeast genetics research
- Extracellular vesicles in disease
- Amoebic Infections and Treatments
- Polyamine Metabolism and Applications
- Trypanosoma species research and implications
- Cytomegalovirus and herpesvirus research
- Zoonotic diseases and public health
- Parasitic Diseases Research and Treatment
- Peptidase Inhibition and Analysis
- Insects and Parasite Interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Child Nutrition and Water Access
- Heat shock proteins research
- Legionella and Acanthamoeba research
- Lysosomal Storage Disorders Research
- Parasites and Host Interactions
- Signaling Pathways in Disease
- Parasitic infections in humans and animals
Instituto de Investigación Médica Mercedes y Martín Ferreyra
2016-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2011-2025
Universidad Nacional de Córdoba
2016-2025
Extracellular vesicles (EVs) facilitate intercellular communication and are considered a promising therapeutic tool for the treatment of infectious diseases. These involve microvesicles (MVs) exosomes selectively transfer proteins, lipids, mRNAs, microRNAs from one cell to another. While MVs formed by extrusion plasma membrane, population endosomal origin that stored inside multivesicular bodies (MVBs) as intraluminal (ILVs) released when MVBs fuse with membrane. Biogenesis may be driven...
The genetically related assemblages of the intestinal protozoa parasite Giardia lamblia are morphologically indistinguishable and often derived from specific hosts. separated by large genetic distances, which might account for their relevant biological pathogenic differences. In this work, we analyzed RNAs cargo released into exosomal-like vesicles (ElVs) A B, differentially infect humans, assemblage E, infects hoofed animals. RNA sequencing analysis revealed that ElVs each contained...
Post-translational modifications of proteins provide cellular physiology with a broad range adaptability to the external environment flexibly and rapidly. In case protozoan parasite Giardia lamblia, study these has gained relevance in recent years, mainly focusing on methylation deacetylation proteins. This investigates significance acetylation this parasite. explores role G. lamblia through combination immunofluorescence assays, manipulation acetyltransferase enzymes, use garcinol, an...
Giardia lamblia, a eukaryotic intestinal parasite, produces small extracellular vesicles (sEVs) as conserved evolutionary mechanism. This study investigates the functional role of sEVs in transferring drug-resistance traits among parasites. derived from metronidazole (MTZ)-resistant clones are shown to modify expression enzymes involved MTZ metabolism and production reactive oxygen species (ROS) recipient wild-type These changes significantly alter drug sensitivity The transfer efficiency...
Introduction Colombian plants have a long history of use in traditional medicine and ethnopharmacology, particularly for treating stomach pain, digestive issues, diarrhea, other gastrointestinal disorders. Recent studies renewed interest their potential therapeutic properties. Methods This study evaluated the giardicidal activity 15 crude plant extracts native to Amazon against Giardia lamblia (genotype A, strain WB/1267). The MTT colorimetric assay was used determine effectiveness these at...
In Giardia, lysosome-like peripheral vacuoles (PVs) need to specifically coordinate their endosomal and lysosomal functions be able successfully perform endocytosis, protein degradation delivery, but how cargo, ligands molecular components generate specific routes the PVs remains poorly understood. Recently, we found that delivering membrane Cathepsin C soluble acid phosphatase (AcPh) is adaptin (AP1)-dependent. However, receptor links AcPh AP1 was never described. We have studied...
Abstract Background To date, eight assemblages of Giardia lamblia have been described, but only A and B are known to infect humans. Despite the fact that genomic, biological, clinical differences found between these two has raised possibility they may be considered different species, there is relatively limited information on their phenotypic differences. In present study, we developed monoclonal antibodies against alpha-1 beta giardin, immunodominant proteins produced during G. infection,...
is a flagellated protozoan parasite causing giardiasis, common intestinal infection characterized by diarrhea, abdominal cramps, and nausea. Treatments employed to combat this parasitic have remained unchanged for the past 40 years, leading emergence of resistant strains prompting search new therapeutic agents.
Abstract The accumulation of unfolded proteins within the Endoplasmic Reticulum (ER) activates a signal transduction pathway termed u nfolded p rotein response (UPR), which attempts to restore ER homeostasis. If homeostasis cannot be restored, UPR signalling ultimately induces apoptosis. Ca 2+ depletion in is potent inducer stress. Despite ubiquity as intracellular messenger, precise mechanism (s) by release affects remains unknown. Use genetically encoded indicator (GCamP6) that tethered...
Epsin N-terminal homology (ENTH) domains are present at the N-terminus of either epsin or epsin-related (epsinR) proteins. These proteins have been involved in clathrin-mediated trafficking and critical for membrane deformation site vesicle budding. While more than one type these described many eukaryotic cells, protozoa parasite Giardia lamblia contains only member this ENTH-protein family. In last two years, four works published showing that giardial protein might play diverse functions....