A5014997750- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Chemistry and Stereochemistry Studies
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Copper Interconnects and Reliability
- Biochemical Analysis and Sensing Techniques
- Photonic and Optical Devices
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Data Visualization and Analytics
Universität Hamburg
2019-2021
Institute of Cell Biophysics
1997
Abstract ARGONAUTE-2 and associated miRNAs form the RNA-induced silencing complex (RISC), which targets mRNAs for translational degradation as part of RNA interference pathway. Despite essential nature this process cellular function, there is little information on role RISC components in human development organ function. We identify 13 heterozygous mutations AGO2 21 patients affected by disturbances neurological development. Each identified single amino acid result impaired shRNA-mediated...
Across phyla, the ribosomes-the central molecular machines for translation of genetic information-exhibit an overall preserved architecture and a conserved functional core. The natural heterogeneity ribosome periodically phases debate on their specialization tissue-specific variations ribosomal protein (RP) pool. Using sensitive differential proteomics, we performed thorough quantitative inventory composition ribosomes from 3 different mouse brain tissues, i.e., hippocampus, cortex,...
In hippocampal neurons of ground squirrels and neuroblastoma culture the ribosome state was analyzed by staining with acridine orange (AO), labeling radioactive amino acids, electron microscopy. Electron microscopy indicated that extent to which ribosomes associated in polysomes varied from 25% brain cells torpid up 93% growing cells. control rat neurons, it 75%. The fluorescence AO-rRNA complex changed polysome/monosome ratio. red AO-single-stranded rRNA as well association decreased...
In translation, G•U mismatch in codon-anticodon decoding is an error hotspot likely due to transition of from wobble (wb) Watson-Crick (WC) geometry, which governed by keto/enol tautomerization (wb-WC reaction). Yet, effects the ribosome on wb-WC reaction and its implications for mechanism remain unclear. Employing quantum-mechanical/molecular-mechanical umbrella sampling simulations using models ribosomal site (A site) we determined that endoergic open, but weakly exoergic closed A-site...
Abstract G○U(T) mismatch has the highest contribution to error rate of base pair recognition in replication, as well codon-anticodon decoding translation. Recently, this effect was unambiguously linked keto-enol tautomerization, which enables Watson-Crick (WC) geometry pair. Structural studies ribosome revealing G○U WC closed state A-site challenge canonical induced-fit model and currently lack a physicochemical explanation. Using computational theoretical methods, we address effects...