A5057703412- Parasites and Host Interactions
- Immune Response and Inflammation
- Gut microbiota and health
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Circadian rhythm and melatonin
- Parasite Biology and Host Interactions
- Immune Cell Function and Interaction
- Advanced Glycation End Products research
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Retinal Development and Disorders
- Helicobacter pylori-related gastroenterology studies
- Eosinophilic Disorders and Syndromes
- Physiological and biochemical adaptations
- NF-κB Signaling Pathways
- Digestive system and related health
- Metallurgy and Material Science
- Eosinophilic Esophagitis
- Immune responses and vaccinations
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive System and Pregnancy
- T-cell and B-cell Immunology
- Animal Behavior and Welfare Studies
University of Manchester
2008-2024
Manchester Academic Health Science Centre
2019-2021
Institute of Immunology
2020
The large intestine is a major site of infection and disease, yet little known about how immunity initiated within this the role dendritic cells (DCs) in process. We used well-established model Trichuris muris to investigate innate response colonic DCs mice that are inherently resistant or susceptible infection. One day postinfection, there was significant increase number immature but not mice. This sustained at 7 postinfection when majority were mature. There no DC numbers until 13...
Laboratory model organisms have provided a window into how the immune system functions. An increasing body of evidence, however, suggests that responses naive laboratory animals may differ substantially to those their wild counterparts. Past exposure, environmental challenges and physiological condition all impact on responsiveness. Chronic infections soil-transmitted helminths, which we define as establishment adult, fecund worms, impose significant health burdens humans, livestock...
Eosinophils are innate immune cells present in the intestine during steady state conditions. An intestinal eosinophilia is a hallmark of many infections and an accumulation eosinophils also observed inflammatory disorders. Classically function has been associated with tissue destruction, due to release cytotoxic granule contents. However, recent evidence demonstrated that eosinophil plays more diverse role system than previously acknowledged, including shaping adaptive responses providing...
Abstract The murine bone marrow has a central role in immune function and health as the primary source of leukocytes adult mice. Laboratory mice provide human-homologous, genetically manipulable reproducible model that enabled an immeasurable volume high-quality immunological research. However, recent research questioned translatability laboratory mouse into humans proposed exposure to their wild natural environment may hold key further breakthroughs. To date, there have been no studies...
Abstract Mucin protein glycosylation is important in determining biological properties of mucus gels, which form protective barriers at mucosal surfaces the body such as intestine. Ecological factors including: age, sex, and diet can change barrier by modulating mucin glycosylation. However, our understanding stems from controlled laboratory studies house mice, combined influence ecological on real-world contexts remains limited. In this study, we used histological staining with ‘Alcian...
The Siberian hamster exhibits the key winter adaptive strategy of daily torpor, during which metabolism and heart rate are slowed for a few hours body temperature declines by up to 20 degrees C, allowing substantial energetic savings. Previous studies hibernators in drops >30 C many days weeks have revealed decreased transcription translation hypometabolism identified several physiological pathways involved. Here we used cDNA microarray define cardiac transcript changes over course torpor...
The intestinal nematode parasite Trichuris muris dwells in the caecum and proximal colon driving an acute resolving inflammation dominated by presence of macrophages. Notably, these macrophages are characterised their expression RELMα during resolution phase infection. RELMα+ macrophage phenotype associates with alternatively activated work other model systems has demonstrated that balance classically is critically important enabling inflammation. Moreover, context type 2 immunity,...
Abstract Background Identifying the factors that contribute to chronicity in inflamed colitic tissue is not trivial. However, mouse models of colitis, we can investigate at preclinical timepoints. We sought validate murine Trichuris muris infection as a model for identification promote development chronic colitis. Methods compared changes mice with resolving immune response T. (resistant) vs fail expel worms and develop colitis (susceptible). Findings were then validated healthy controls...
ABSTRACT For decades, parasitic worms such as Trichuris muris have been maintained in laboratory animals, providing insights into host–parasite interactions and host immune responses. The most used T. isolate is the E isolate, established 1954. However, one concern with these model systems potential for laboratory‐induced selection therefore changes interactions. To address concerns, we compare a recently isolated (M isolate), from wild house mice ( Mus musculus domesticus , Isle of May,...
Isotype control staining. Representative figures showing isotype staining in small and large intestine. Scale Bar represents 100Â Iźm. (PDF 2178Â kb)
Abstract Laboratory model organisms have provided a window into how the immune system functions. An increasing body of evidence, however, suggests that responses naive laboratory animals may differ substantially to those their wild counterparts. Past exposure, environmental challenges and physiological condition all impact on responsiveness. Chronic infections soil-transmitted helminths impose significant health burdens humans, livestock wildlife, with limited treatment success. In mice, Th1...
Abstract Mucin protein glycosylation is important in determining biological properties of mucus gels, which form protective barriers at mucosal surfaces the body such as intestine. Ecological factors including: age, sex, and diet can change barrier by modulating mucin glycosylation. However, our understanding stems from controlled laboratory studies house mice, combined influence ecological on real-world contexts remains limited. In this study, we used histological staining with ‘Alcian...
<h3>Introduction</h3> Up to 40% of patients with inflammatory bowel disease (IBD) also suffer osteoporosis.1 Receptor activator NF-kB ligand (RANKL) promotes the formation bone resorbing osteoclasts and is linked osteoporosis.2 RANKL controlled by decoy receptors, osteoprotegerin (OPG) TNF-stimulated gene-6 (TSG6) that bind it block osteoclast resorption.2 We have shown increased serum levels in a mouse model colitis disease.3 may increase lifespan activity dendritic cells (DCs), which...
Abstract Aims Identifying the factors that contribute to chronicity in inflamed colitic tissue is not trivial. However, mouse models of colitis, we can investigate at preclinical timepoints. We sought validate murine Trichuris muris infection as a model for identification promote development chronic colitis. Methods compared changes mice with resolving immune response T. (resistant) versus fail expel worms and develop colitis (susceptible). Findings were then validated healthy controls...
ABSTRACT The balance of pro-inflammatory and anti-inflammatory macrophages is critically important in enabling the development resolution inflammatory responses. Anti-inflammatory have been shown to be activated by IL4 and/or IL13 via IL4Rα. In context type 2 immunity, defined expression signature markers RELMα, CD206 Ym1, associated with activation Despite a breadth pathologies large intestine, many which feature unbalanced macrophage states, little known about how intestinal are activated....