A5020619787- Lysosomal Storage Disorders Research
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
- Neurogenetic and Muscular Disorders Research
- Immunotoxicology and immune responses
- Carcinogens and Genotoxicity Assessment
- Glycogen Storage Diseases and Myoclonus
- Natural product bioactivities and synthesis
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Bioactive Compounds and Antitumor Agents
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Synthesis and Biological Evaluation
- Vanadium and Halogenation Chemistry
- Virus-based gene therapy research
- Immune cells in cancer
- CRISPR and Genetic Engineering
- Eicosanoids and Hypertension Pharmacology
- Cystic Fibrosis Research Advances
- RNA modifications and cancer
- Estrogen and related hormone effects
- Biochemical and Molecular Research
ProterixBio (United States)
2018-2024
TU Wien
2023
Takeda (United States)
2010-2011
Research Institute for Genetic and Human Therapy
2011
University of Pittsburgh
2010
Novartis (Switzerland)
1993-1994
Purdue University West Lafayette
1987-1991
Hochschule Aalen
1990
National Institutes of Health
1986
National Institute of Environmental Health Sciences
1985
TBK1 is critical for immunity against microbial pathogens that activate TLR4- and TLR3-dependent signaling pathways. To address the role of in inflammation, mice were generated harbor two copies a mutant Tbk1 allele. This Tbk1(Δ) allele encodes truncated protein catalytically inactive expressed at very low levels. Upon LPS stimulation, macrophages from Tbk1(Δ/Δ) produce normal levels proinflammatory cytokines (e.g., TNF-α), but IFN-β RANTES expression IRF3 DNA-binding activity are ablated....
Recombinant human idursulfase, an intravenous enzyme replacement therapy indicated for treatment of somatic symptoms mucopolysaccharidosis II (Hunter syndrome), is anticipated to have minimal benefit the cognitive impairment associated with severe phenotype. Because intrathecal (IT) administration other lysosomal disorders has shown efficacy in animal models, IT formulation idursulfase (idursulfase-IT) and a drug-delivery device (subcutaneous port connected lumbar catheter) were developed...
Soluble immune response suppressor (SIRS) is a product of concanavalin A-stimulated murine T cells that, when activated or oxidized by macrophages H2O2 (SIRSox), suppresses in vitro responses and inhibits cell division normal neoplastic cells. SIRSox inactivated variety electron donors, which suggests that may be an oxidizing agent. Incubation lymphocytes with SIRSox, but not SIRS, partially reversed A-mediated inhibition capping membrane immunoglobulin on B cells, disrupted the cytoplasmic...
The effect of horse-chestnut seed extract (standardized on aescin; Venostasin retard) was assessed in a randomized placebo-controlled crossover double-blind trial 22 patients with proven chronic venous insufficiency by measuring the capillary filtration coefficient and intravascular volume lower leg venous-occlusion plethysmography. Three hours after taking two capsules (600 mg; each capsule containing 50 mg aescin) had decreased 22%, whereas administration an identical-looking placebo it...
Gaucher disease type 1 (GD1) is an inherited lysosomal disorder with multisystemic effects in patients. Hallmark symptoms include hepatosplenomegaly, cytopenias, and bone varying degrees of severity. Mutations a single gene, glucosidase beta acid (GBA1), are the underlying cause for disorder, resulting insufficient activity enzyme glucocerebrosidase, which turn leads to progressive accumulation lipid component glucocerebroside. In this study, we treat mice signs consistent GD1, hematopoietic...
Pompe disease is a rare genetic neuromuscular disorder caused by acid α-glucosidase (GAA) deficiency resulting in lysosomal glycogen accumulation and progressive myopathy. Enzyme replacement therapy, the current standard of care, penetrates poorly into skeletal muscles peripheral central nervous system (CNS), risks recombinant enzyme immunogenicity, requires high doses frequent infusions. Lentiviral vector-mediated hematopoietic stem progenitor cell (HSPC) gene therapy was investigated mouse...
Abstract Local production of reactive oxygen intermediates, e.g., superoxide anion, by tumor promoter—stimulated inflammatory macrophages (MPs) may contribute significantly to development in classical models two‐stage chemical‐induced carcinogenesis murine skin. In the studies reported herein, peritoneal MPs elicited from phorbol‐ester—sensitive SENCAR mice demonstrated a time‐ and dose‐dependent release anion (4–6 nmol/10 6 cells) when stimulated 200 nM 12‐ O...
Journal Article Induction of interleukin-1 and tumor necrosis factor by 12-O-tetradecanoylphorbol-13-acetate in phorbol ester-sensitive (SENCAR) resistant (B6C3F1) mice Get access Lawrence W. Updyke, Updyke Department Pharmacology Toxicology, School Pharmacy Pharmacological Sciences, Purdue UniversityWest Lafayette, IN 47907, USA Search for other works this author on: Oxford Academic PubMed Google Scholar Hyunah L. Yoon, Yoon Anchalee Chuthaputti, Chuthaputti Richard Pfeifer, Pfeifer 1 1To...
Pharmacological doses of estrogens such as 17-beta estradiol (17- beta E) and diethylstilbestrol (DES) activate macrophages in a thymic-dependent manner vivo. In this report, we investigated the direct vitro effects 17- E its major metabolites on macrophage activation response to lectin-stimulated lymphocyte supernatants containing macrophage-activating factor (MAF), T cell lymphokine (LK). Activation was measured terms cytostasis against cultured tumor cells. As suggested by previous...
Following two weeks of topical application 12-O-tetradecanoylphorbol-13-acetate (TPA) at 2, 4 and 8 micrograms/mouse on alternate days (7X total) or benzoyl peroxide (BZP) 10, 20 40 mg/mouse, natural killer (NK) activity was determined in local (lymph nodes draining the lower dorsal region) systemic (spleen) lymphoid tissue phorbol ester-sensitive (SENCAR) resistant (B6C3F1) mice. SENCAR mice, sensitive to tumor induction by TPA two-stage chemical-induced carcinogenesis protocols,...
Abstract Background Selective neutralization of the IL21/IL21R signaling pathway is a promising approach for treatment variety autoimmune diseases. Ab-01 human neutralizing anti-IL21R antibody. In order to ensure that activities are restricted even under in vitro cross-linking and vivo conditions, comprehensive assessment agonistic potential was undertaken. Methods antibody cell culture protocols reported studies with antibody, TGN1412, were followed Ab-01. rhIL21, agonist ligand targeted...
Previous studies in this laboratory and others have indicated that 12-O-tetradecanoylphorbol-13-acetate (TPA), the most potent skin tumor promoter known, evokes significantly greater inflammatory responses phorbol ester-sensitive (SENCAR) mice compared to strains (e.g. C57BL/6 B6C3F1) are relatively resistant outgrowth of tumors two-stage chemically induced carcinogenesis protocols. In reported herein, subchronic topical application TPA at doses relevant protocols serum...
This article looks at public anti-restriction protests by framing space as a vital component of urban life. It argues that the body is rarely introduced scale spatial analysis and usually plays more prominent role in subfields social movement or studies, which often tend to focus on transformative emancipatory side encounters. By integrating corporeal perspective, aims understanding how transforms political passions into individual agency collective action. Focusing Covid-19-crisis-related...