A5070412458- Immunotoxicology and immune responses
- Animal testing and alternatives
- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Glycogen Storage Diseases and Myoclonus
- Lysosomal Storage Disorders Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Pain Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Cerebrospinal fluid and hydrocephalus
- Carcinogens and Genotoxicity Assessment
- Cell Image Analysis Techniques
- Paraquat toxicity studies and treatments
- Botulinum Toxin and Related Neurological Disorders
- Cellular transport and secretion
- Amyotrophic Lateral Sclerosis Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological disorders and treatments
- Wheat and Barley Genetics and Pathology
- Reproductive System and Pregnancy
- Glioma Diagnosis and Treatment
- Peripheral Neuropathies and Disorders
- Genetics and Plant Breeding
- Stress Responses and Cortisol
South Australian Research and Development Institute
2022-2024
Atlas Copco (United States)
2014-2022
Walker (United States)
2008-2019
Ultragenyx Pharmaceutical (United States)
2016
MPI Research (United States)
2016
BioMarin (United States)
2016
Pfizer (United States)
2014
National Cancer Institute
2013
Advanced Targeting Systems (United States)
2013
Charles River Associates
2006-2007
The Society of Toxicologic Pathology charged a Nervous System Sampling Working Group with devising recommended practices to routinely screen the central nervous system (CNS) and peripheral (PNS) in Good Laboratory Practice–type nonclinical general toxicity studies. Brains should be weighed trimmed similarly for all animals study. Certain structures sampled regularly: caudate/putamen, cerebellum, cerebral cortex, choroid plexus, eye (with optic nerve), hippocampus, hypothalamus, medulla...
Harmonization of diagnostic nomenclature used in the pathology analysis tissues from rodent toxicity studies will enhance comparability and consistency data sets different laboratories worldwide. The INHAND Project ( International Nomenclature Diagnostic Criteria for Lesions Rats Mice) is a joint initiative four major societies toxicologic to develop globally recognized proliferative nonproliferative lesions rodents. This article recommends standardized terms classifying changes observed...
The neurotoxicity of paraquat dichloride (PQ) was assessed in two inbred strains 9- or 16-week old male C57BL/6 mice housed different laboratories and compared to the effects 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). PQ administered by intraperitoneal injections; either once (20 mg/kg) twice (10 weekly for 3 weeks, while MPTP-HCl injected 4 times on a single day mg/kg/dose). Brains were collected 8, 16, 24, 48, 96 168 hours after last treatment, 48 MPTP treatment. Dopamine neurons...
Recent studies in non-human primates administered recombinant adeno-associated viruses (rAAVs) have shown lesions the dorsal root ganglia (DRG) of unknown pathogenesis. In this study, rAAV9s manufactured using different purification methods alongside a non-expressing Null AAV9 vector was to groups cynomolgus monkeys followed by neuropathological evaluation after 4 weeks. Lesions, including neuronal degeneration, increased cellularity, and nerve fiber were observed DRG, regardless methods....
Recombinant human glial cell line-derived neurotrophic factor (r-metHuGDNF) is a potent neuronal growth and survival that has been considered for clinical use in the treatment of Parkinson’s disease (PD). Here we present results 6-month toxicology study rhesus monkeys conducted to support evaluation chronic intraputamenal infusion r-metHuGDNF PD. Monkeys (6–9/sex/group) were treated with 0 (vehicle), 15, 30, or 100 μg/day by continuous unilateral (150 μl/day flow rate) 6 months; subset...
The use of adjuvants in regional anesthesia has increased. However, there are knowledge gaps pertaining to 1) vivo local tissue effects these adjuvants; and 2) chemical compatibility solubility drugs solution with each other anesthetics. This study addresses knowledge.In rat safety/toxicopathology analytical chemistry study.Collaborating Good Laboratory Practice laboratories under the direction university-based principal investigator.Single-injection formulations clonidine, buprenorphine,...
Peripheral nervous system (PNS) toxicity is surveyed inconsistently in nonclinical general studies. These Society of Toxicologic Pathology “best practice” recommendations are designed to ensure consistent, efficient, and effective sampling, processing, evaluation PNS tissues for four different situations encountered during (screening) dedicated neurotoxicity For studies where unknown or not anticipated (situation 1), may be limited one sensorimotor spinal nerve. If somatic suspected 2),...
The pharmacokinetics and neurotoxicity of paraquat dichloride (PQ) were assessed following once weekly administration to C57BL/6J male mice by intraperitoneal injection for 1, 2 or 3 weeks at doses 10, 15 25 mg/kg/week. Approximately 0.3% the administered dose was taken up brain slowly eliminated, with a half-life approximately weeks. PQ did not alter concentration dopamine (DA), homovanillic acid (HVA) 3,4-dihydroxyphenylacetic (DOPAC), increase turnover in striatum. There inconsistent...
Crown rot, caused by a stubble-soil fungal pathogen, threatens wheat production, leading to significant yield losses. The challenge lies in the absence of early visible symptoms for timely detection and management. While hyperspectral imaging technologies have been successfully applied improve screening some plant health indicators diseases, they not yet used crown rot disease detection. This study endeavoured harness early-stage, high-throughput, accurate, economical, non-destructive...
Summary Cytologic samples of popliteal lymph node, proximal femoral bone marrow, and the buffy coat fraction blood were obtained from 56 dogs. The number mast cells on 1 slide each sample was determined by microscopic examination. Eleven 46 slides node aspirate contained (range, to 16; mean, 6.4; median, 5 cells/slide). Fifty-one marrow evaluated. Two these a single cell. None 53 smear examined any cells. These results indicated that in clinically normal dogs, few several may be encountered...
Recombinant human idursulfase, an intravenous enzyme replacement therapy indicated for treatment of somatic symptoms mucopolysaccharidosis II (Hunter syndrome), is anticipated to have minimal benefit the cognitive impairment associated with severe phenotype. Because intrathecal (IT) administration other lysosomal disorders has shown efficacy in animal models, IT formulation idursulfase (idursulfase-IT) and a drug-delivery device (subcutaneous port connected lumbar catheter) were developed...
A retrospective analysis of microscopic evaluation data from control (device and/or saline-treated) animals in intrathecal studies monkeys, dogs, sheep, and rats was conducted. The were performed by multiple testing facilities. All slide preparation conducted the laboratory author. observations made at level catheter tip, which typically located space near thoracolumbar region spinal canal. most common changes meningeal infiltrates, track (CT) inflammation, cord compression (at CT), CT...
Abstract Background: Neurokinin-1 receptors (NK1-rs) located on superficial dorsal horn neurons are essential for integration of nociceptive input. Intrathecal injection substance P-saporin (SP-SAP) leads to local loss spinal NK1-r (+) suggesting its potential as a therapeutic agent chronic pain. The authors determined, in canine model, effects lumbar intrathecal SP-SAP. Methods: Distribution SP-SAP and Saporin was determined plasma, cerebrospinal fluid, tissue. Safety four groups (six dogs...
The ability to differentiate among normal structures, procedural and processing artifacts, spontaneous background changes, test article–related effects in the peripheral nervous system (PNS) is essential for interpreting microscopic features of ganglia nerves evaluated animal species commonly used toxicity studies evaluating regulated products chemicals. This atlas provides images findings that may be encountered product discovery development. Most are tissues from control animals were...
Harmonization of diagnostic terminology used during the histopathologic analysis rodent tissue sections from nonclinical toxicity studies will improve consistency data sets produced by laboratories located around world. The INHAND Project ( International Nomenclature and Diagnostic Criteria for Lesions in Rats Mice) is a cooperative enterprise 4 major societies toxicologic pathology to develop globally accepted standard vocabulary proliferative nonproliferative lesions rodents. A prior...
A laboratory cat died 12 days after intraperitoneal inoculation of a 1 ml suspension containing 1.5 x 10(6) blood mononuclear cells from Florida panther (Felis concolor coryi). Gross, histologic and ultrastructural investigations revealed the cause death to be infection by Cytauxzoon felis, protozoal parasite known rapidly fatal disease (cytauxzoonosis) in domestic cats. The bobcat rufus) has been identified as natural host for C. felis. This report implicates another possible
Neuropathology analyses as end points during nonclinical efficacy and toxicity studies are challenging require trained personnel particular equipment to achieve optimal results. Accordingly, many regulatory agencies have produced explicit guidelines for designing performing neuropathology assessments studies. This compilation of international guidance toxicologic represents a set criteria recommended general specialized neurotoxicity that should facilitate the efforts individuals who plan,...
Tanezumab, a humanized monoclonal antibody against nerve growth factor is in development for treatment of chronic pain. Three nonclinical studies assessed effects clinically relevant and supratherapeutic doses tanezumab on the sympathetic nervous system (SNS) adult nonhuman primates. Study 1 evaluated potential subcutaneous (SC) (1.2 mg/kg every 8 weeks [Q8W]) SNS cynomolgus monkeys 3 or 6 months reversibility persistence any through nondosing/recovery period. 2 whether neuronal cell death...