The promotion of sugarcane growth by the endophytic Pantoea agglomerans strain 33.1 was studied under gnotobiotic and greenhouse conditions. green fluorescent protein (GFP)-tagged P. 33.1::pNKGFP monitored in vitro plants microscopy, reisolation, quantitative PCR (qPCR). Using qPCR reisolation 4 15 days after inoculation, we observed that GFP-tagged strains reached similar density levels both rhizosphere inside roots aerial plant tissues. Microscopic analysis performed at 5, 10, 18...

Summary The beneficial features of Bacillus thuringiensis ( Bt ) are not limited to its role as an insecticide; it is also able promote plant growth interacting with plants and other growth‐promoting rhizobacterium (PGPR). PGPR strain RZ2MS9 a multi‐trait maize promoter. We obtained stable mutant labelled green fluorescent protein (RZ2MS9‐GFP). demonstrated that the RZ2MS9‐GFP successfully colonizes maize's roots leaves endophytically. evaluated whether has additive effect on promotion when...

The chromosomes of Crotalaria juncea, a legume agronomic interest with 2n = 16 karyotype composed metacentric chromosomes, were analyzed using several cytogenetic techniques. C-banding revealed heterochromatic regions around the centromeres in all and adjacent to secondary constriction on chromosome 1 short arm. Fluorescent staining GC-specific chromomycin A3 (CMA) highlighted these tiny site long arm while AT-specific stain 4'-6-diamidino-2-phenylindole (DAPI) induced reversed pattern....

Abstract — The present study was carried out to analyze the karyotypes of three Passiflora species with 2n = 18 (the yellow passion fruit P. edulis f. flavicarpa and wild amethystina cincinnata) previously used obtain somatic hybrids by protoplast fusion. A biometrical analysis Feulgen stained metaphases showed that these were quite similar subtle differences in morphology some chromosomes between species. These data integrated physical mapping 45S rDNA (18S-5.8S-26S rRNA genes) 5S loci...

The Cattleya (Orchidaceae–Laeliinae subtribe) intergeneric hybrids, such as Brassolaeliocattleya (Blc.), have great ornamental value, due to their compact-size, with large and high color diversity of flowers. Artificial induction polyploidy brings agronomic, genetic benefits plants. Polyploidization efficiency depends on factors, the type antimitotic, polyploidization method, concentrations, exposure times explant. This study aimed develop a protocol polyploidize Blc. orchids, by testing two...

Ratoon stunting disease ( RSD ), caused by the bacterium Leifsonia xyli subsp. (Lxx), is one of most economically important diseases sugarcane worldwide. Because knowledge on interaction Lxx with its host at microscopic level limited, development tools to monitor during colonization process could shed new light processes that control development. In this investigation, a transformation protocol was optimized and mutant strain engineered stably expressed gfp gene in tissues. vitro , growth...

We report the in vitro induction of polyploids from two cassava cultivars by colchicine treatment. Shoot nodal segments, collected cultivated plants Porquinho and Vassourinha cultivars, were used as explants. Explants treated liquid media without with (0.05, 0.10 or 0.15%), at 90 rpm, dark, for 48 96 h. Normal-like putative-polyploid phenotypes recovered culture, both cultivars. All group confirmed tetraploids flow cytometry root tip chromosome counting, whereas no association was found...

Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes improved with the development fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified sister inbred lines that were derived from a tropical flint population (Jac Duro [JD]), hybrids between them, FISH probes for 180-bp knob repeat, centromeric satellite (CentC), 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal...

Abstract The modern cultivars of sugarcane ( Saccharum spp.) are highly polyploid and accumulate aneuploidies due to their history domestication, genetic improvement interspecific hybrid origin involving the domesticated sweet species officinarum (‘noble cane’) wild spontaneum , both with an evolutionary polyploidy. first hybrids were backcrossed S. selection from progenies in subsequent generations established basis cultivars. genome complexity has inspired several molecular studies that...

Most Crotalaria species display a symmetric karyotype with 2n = 16, but 14 is found in Chrysocalycinae subsection Incanae and 32 American of the section Calycinae. Seven sections Chrysocalycinae, Calycinae, were analyzed for identification heterochromatin types GC- AT-specific fluorochromes chromosomal location ribosomal DNA loci using fluorescent situ hybridization (FISH). A major 45S rDNA locus was observed on chromosome 1 all species, variable number minor ones revealed. Only one 5S...

Introduction Soybean is a significant export product for several countries, including the United States and Brazil. There are numerous varieties of soybean. Among them, genetically modified type known as INTACTA RR2 PRO™ has been designed to demonstrate resistance glyphosate produce toxins that lethal species caterpillars. Limited information available on use Trichoderma harzianum Bacillus subtilis promote plant growth their impact microbiome. Methods This study aimed evaluate effects these...

Foram conduzidos experimentos visando à otimização de procedimentos para preparações citológicas pontas raiz com alta freqüência metáfases mitóticas, apresentando cromossomos morfologia nítida, em espécies pertencentes a dois gêneros plantas, Passiflora (Passifloraceae) e Crotalaria (Leguminosae). Para o acúmulo metáfases, bem como prófases prometáfases condensados, foram avaliados pré-tratamentos raízes, que utilizadas diferentes combinações 8-hidroxiquinolina, inibidor do fuso mitótico,...

Abstract Meiosis and pollen viability of interspecific somatic hybrids, i.e. Passiflora edulis f. flavicarpa , the yellow passion fruit + P. cincinnata a wild species, were examined compared with their corresponding diploid fusion parents. Firstly, cybrid nature these hybrids was confirmed by leaf‐stomatal measurements RFLP analyses using four mitochondrial genes as probes. The meiotic behaviour revealed relatively high stability, most hybrid cells showing 18 bivalents. Some instability,...

Malpighia emarginata Sessé & Mociño ex DC. or West Indian cherry (acerola) is a wild plant originated in southern Mexico, Central America and the northern region of South America. The species was introduced to Brazil about 60 years ago now country world's biggest producer. Even though fruits acerola have high commercial value, as they are an important source natural vitamin C, very little chromosome information available for this species. Previous studies showed that most diploids,...

Abstract Euschistus stink bugs are important pests on soybean crops, including the Neotropical representatives heros, crenator, and taurulus. Despite their importance, little genomic chromosomal information is available. Genomic differences can play an role in establishing reproductive barriers between species populations. Here we investigate chromosomes, some repetitive DNAs, genome sizes of three species, two E. heros strains (North strain-NS South strain-SS), to address evolution...

Com o objetivo de estudar a variabilidade genética intra-específica em Oncidium varicosum Lindl. (Orchidaceae - Oncidiinae), foram avaliadas cinco populações Minas Gerais, coletadas Alfenas (AL), Estiva (ES), Pedra do Coração (PC), Branca (PB) e Pouso Alegre (PA). Foram considerados 22 caracteres florais, medidos partir "vouchers" (fichas florais), empregando-se métodos estatísticos univariados multivariados (análise discriminante canônica CDA análise agrupamento distância Mahalanobis)....