Nineteen strains of lactic acid bacteria were investigated for antioxidative activity. These included Lactobacillus acidophilus B, E, N1, 4356, LA-1, and Farr; bulgaricus 12 278, 448, 449, Lb, 1006, 11 842; Streptococcus thermophilus 821, MC, 573, 3641, 19 987; Bifidobacterium longum B6 15 708. Intracellular cell-free extract all demonstrated activity with inhibition rates ascorbate autoxidation in the range 7−12%. Antioxidative mechanisms including metal ion chelating ability, scavenge...

Heat-killed lactic acid bacteria not only possess immunomodulatory functions but also provide the advantages of longer product shelf life, easier storage, and more convenient transportation. To establish appropriate heat treatments for industrial preparation probiotics with effects, 4 different were used to kill 11 strains bacteria. Comparisons among viable forms carried out in terms activity adhesion Caco-2 cells. Field-emission scanning electron microscope (FE-SEM) was employed observe...

There are close links between chemotherapy-induced intestinal mucositis and microbiota dysbiosis. Previous studies indicated that D-methionine was an excellent candidate for a chemopreventive agent. Here, we investigated the effects of on cisplatin-induced mucositis.Male Wistar rats (176-200 g, 6 weeks old) were given cisplatin (5 mg/kg) treated with (300 mg/kg). Histopathological, digestive enzymes activity, oxidative/antioxidant status, proinflammatory/anti-inflammatory cytokines in...

Cisplatin-induced nephrotoxicity is associated with gut microbiota disturbance. The present study aimed to investigate whether supplementation of Lactobacillus reuteri and Clostridium butyricum (LCs) had a protective effect on cisplatin-induced through reconstruction microbiota. Wistar rats were given different treatments: control, cisplatin (Cis), + C. L. (Cis+LCs), (LCs). We observed that cisplatin-treated supplemented LCs exhibited significantly decreased renal inflammation (KIM-1, F4/80,...

Cholesterol reducing abilities of six strains Lactobacillus acidophilus including ATCC 4356, B, E, Farr, LA-1 and N-1 were investigated in vitro. All these able to reduce cholesterol at various levels the broth system used this study. Among tested, L. 4356 demonstrated best ability 57 71% presence oxgall cholic acid, respectively, broth. Results from acid tolerance growth studies that would likely survive human gastrointestinal tract, where are present stomach small intestine....

It is believed that probiotics play an important role for the health of host, including modulation immune responses. Most studies have focused on immunomodulatory effects viable cells lactic acid bacteria; however, we investigated those heat-killed bacteria in this study. We first observed functions via stimulating splenocytes with three Lactobacillus strains. Furthermore, also effect mouse dendritic (DCs) treated these strains T cell The results showed were able to stimulate proliferation...

The inhibition of lipid peroxidation by Lactobacillus acidophilus and Bifidobacterium longum was investigated using two model systems. All eight strains, including six strains L. B. longum, demonstrated an inhibitory effect on linoleic acid peroxidation. rates ranged from 33 to 46% when 1 mL intracellular cell-free extract tested. In the second system, cell membrane osteoblast used as source for biological lipid. results indicated that all were able protect lipids oxidation. 22 37%....

Abstract Background Crohn's disease and ulcerative colitis are the major types of chronic inflammatory bowel occurring in colon small intestine. A growing body research has proposed that probiotics able to attenuate symptoms these diseases vitro vivo . However, mechanism probiotic actions remains unclear. Results Our results suggested Lactobacillus plantarum MYL26 inhibited inflammation Caco-2 cells through regulation gene expressions TOLLIP , SOCS1 SOCS3 IκBα rather than SHIP-1 IRAK-3...

Abstract Telomerase expression is the hallmark of tumor cells, and activation this ribonucleoprotein complex may be a rate‐limiting or critical step in cellular immortalization oncogenesis. Fungal immunomodulatory protein, FIP‐gts, has been isolated from Ganoderma tsugae . In present study, we expressed purified recombinant fungal protein reFIP‐gts E. coli. We found that significantly selectively inhibits growth A549 cancer cells while not affecting normal MRC‐5 fibroblasts. The suppression...

Abstract Fungal immunomodulatory proteins (FIPs) are a group of novel proteins, purified from medicinal fungi or edible mushrooms that possess properties. FIP-gts and FIP-fve have been isolated Ganoderma tsugae Flammunlina velutipes, respectively. The evaluation FIP activity was based on their ability to stimulate human peripheral blood lymphocytes release interferon-gamma (IFN-γ). We found exhibited better than FIP-fve. Activities were both greatly reduced with duration heating. For...

The objective of this study was to assess the antiallergic effect fermented milk prepared, respectively, with Streptococcus thermophilus MC, Lactobacillus acidophilus B, bulgaricus Lb, L. 448, and Bifidobacterium longum B6. Female BALB/c mice fed were immunized intraperitoneally ovalbumin (OVA)/complete Freund's adjuvant (CFA) evaluate immune response by observing secretion cytokines IL-2, IL-4, IFN-gamma serum antibody IgE. results showed that supplementation lactic acid bacteria did not...

Cisplatin is a widely used antineoplastic drug. However, cisplatin-induced dyspepsia syndromes, including delayed gastric emptying, distension, early satiety, nausea, and vomiting, often force patients to take doses lower than those prescribed or even refuse treatment. D-methionine has an appetite-enhancing effect alleviates weight loss during cisplatin treatment.This work established model of anorexia symptoms with intraperitoneal injection (5 mg/kg) once week for three cycles....