A5018474833- Machine Learning in Bioinformatics
- Bioinformatics and Genomic Networks
- Computational Drug Discovery Methods
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Infection Control in Healthcare
- Infection Control and Ventilation
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Birth, Development, and Health
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- COVID-19 diagnosis using AI
- Antimicrobial Resistance in Staphylococcus
- RNA Research and Splicing
- SARS-CoV-2 detection and testing
- Dental Research and COVID-19
- Quality and Safety in Healthcare
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Metabolic Engineering and Bioproduction
- Antibiotic Use and Resistance
Netaji Subhas University of Technology
2010-2025
Central Texas Veterans Health Care System
2019-2025
Olin E. Teague Veterans Medical Center
2024
Medscape
2015-2023
Communities In Schools of Orange County
2022
University of Richmond
2022
Oregon Medical Research Center
2022
AMRI Hospitals
2017-2022
New York Proton Center
2016-2022
Lindsay Unified School District
2022
AimsElevated levels of pro-inflammatory cytokine interleukin-17A (IL-17) are associated with hypertensive autoimmune diseases; however, the connection between IL-17 and hypertension is unknown. We hypothesized that increases blood pressure by decreasing endothelial nitric oxide production.
Preeclampsia (PE) is a pregnancy-specific hypertensive syndrome characterized by excessive maternal immune system activation, inflammation, and endothelial dysfunction. Toll-like receptor (TLR) 3 activation double-stranded RNA (dsRNA) TLR7/8 single-stranded (ssRNA) expressed viruses and/or released from necrotic cells initiates pro-inflammatory response; however it unknown whether viral/endogenous key initiating signal that contributes to the development of PE. We hypothesized TLR3/7/8 will...
Preeclampsia may result from overactivation of the maternal immune system and is characterized by endothelial dysfunction excessive inflammation. Given importance regulation anti-inflammatory cytokines in normotensive pregnancies, we hypothesized that activation via Toll-like receptor 3 during pregnancy would cause preeclampsia-like symptoms mice, which be made worse deficiency cytokine interleukin 10. The agonist polyinosine-polycytidylic acid (poly I:C) caused hypertension, dysfunction,...
Abstract Protein-protein interactions (PPI) control most of the biological processes in a living cell. In order to fully understand protein functions, knowledge protein-protein is necessary. Prediction PPI challenging, especially when three-dimensional structure interacting partners not known. Recently, novel prediction method was proposed by exploiting physical constituent domains. We propose here knowledge-based method, namely PPI_SVM, which predicts between two sequences their domain...
Patients treated with the immunosuppressive drug tacrolimus (FK506), which binds FK506 binding protein 12 (FKBP12) and then inhibits calcium-dependent phosphatase calcineurin, exhibit decreased regulatory T cells, endothelial dysfunction, hypertension; however, mechanisms whether altered T-cell polarization play a role are unknown. Tacrolimus treatment of mice for 1 week dose-dependently splenic CD4(+)/FoxP3(+) (regulatory cells), increased CD4(+)/IL-17(+) (T-helper 17) caused dysfunction...
Excessive maternal immune system activation plays a central role in the development of hypertensive disorder pregnancy preeclampsia (PE). The immunomodulatory cytokines interleukin 4 (IL-4) and 10 (IL-10) are dysregulated during PE; therefore we hypothesized that treatment with both recombinant IL-4 IL-10 could prevent PE mice.Using our mouse model which is induced by double-stranded RNA receptor agonist poly I:C, gave daily injections IL-4, IL-10, or on days 13-17 pregnancy. Mice were then...
Several clinical studies have reported increased placental miR-210 expression in women with PE compared to normotensive women, but whether plays a role the etiology of is unknown. We that activation TLR3 produces PE-like symptoms hypertension, endothelial dysfunction, and proteinuria mice only when pregnant, pregnant human cytotrophoblasts (CTBs) increases modulates its targets related inflammation are Placental levels were significantly treated agonist poly I:C (P-PIC). Both HIF-1α...
Treatment of pregnancy-related hypertensive disorders, such as preeclampsia (PE), remain a challenging problem in obstetrics. Typically, aggressive antihypertensive drug treatment options are avoided to prevent pharmacological-induced hypotension. Another major concern administering drugs during pregnancy is possible adverse fetal outcome. In addition, management hypertension chronic patients or with prior kidney problems carefully considered. Recent studies suggest that PE at increased...
Abstract Background Despite increasing use of fidaxomicin for Clostridioides difficile infection (CDI), isolates with reduced susceptibility (minimum-inhibitory concentration [MIC] >2 ug/mL) have been rare in surveillance studies. However, there has recent reports clinical associated mutations the RpoB and RpoC genes coding RNA polymerase target fidaxomicin. The implications are uncertain because achieves high concentrations intestinal tract. Whole genome sequencing analysis...
There have been several recent reports of Clostridioides difficile infection (CDI) due to isolates with reduced fidaxomicin susceptibility (minimum inhibitory concentration [MIC] ≥ 2 µg/mL). However, the clinical implications are uncertain because achieves high concentrations in intestinal tract. In an acute care hospital, we conducted a 3-year cohort study patients CDI determine frequency and impact on response treatment. Stool specimens were cultured for C. difficile, testing was performed...
We determined the crystal structure of a bifunctional group I intron splicing factor and homing endonuclease, termed -Ani maturase, in complex with its DNA target at 2.6 Å resolution. The demonstrates remarkable structural conservation β-sheet DNA-binding motif between highly divergent enzyme subfamilies. recognition by I- Ani was further studied using nucleoside deletion DMS modification interference analyses. Correlation these results provides information on relative importance individual...
The gamma interferon (IFN-γ)-activated inhibitor of translation (GAIT) complex in human myeloid cells is heterotetrameric, consisting glutamyl-prolyl-tRNA synthetase (EPRS), NS1-associated protein 1 (NSAP1), ribosomal L13a, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). binds a structural GAIT element the 3′ untranslated region VEGF-A other inflammation-related transcripts inhibits their translation. EPRS dually phosphorylated by cyclin-dependent kinase 5 (Cdk5) at Ser886 then...
Cardiac fibrosis occurs because of disruption the extracellular matrix network leading to myocardial dysfunction. Angiotensin II has been implicated in development cardiac fibrosis. Recently, microRNAs have identified as an attractive target for therapeutic intervention pathologies; however, underlying mechanism remains unclear. MicroRNA-130a (miR-130a) shown participate angiogenesis and arrhythmia; its role is unknown.In this study, we found that miR-130a was significantly upregulated...
Health care personnel and patients are at risk to acquire severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in health settings, including outpatient clinics ancillary areas.