A5077144680- Legionella and Acanthamoeba research
- Nosocomial Infections in ICU
- Escherichia coli research studies
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
- Water Treatment and Disinfection
- Data-Driven Disease Surveillance
- Pneumonia and Respiratory Infections
- Healthcare Facilities Design and Sustainability
- Biosensors and Analytical Detection
- Carbohydrate Chemistry and Synthesis
- Antibiotic Use and Resistance
- Zoonotic diseases and public health
- Bacterial biofilms and quorum sensing
- Vaccine Coverage and Hesitancy
- Disaster Response and Management
- Monoclonal and Polyclonal Antibodies Research
- Patient Satisfaction in Healthcare
- Uterine Myomas and Treatments
- Travel-related health issues
- Public Health Policies and Education
University of Cincinnati Medical Center
2013-2024
United States Department of Veterans Affairs
2013-2024
Veterans Health Administration
2013-2024
University of Cincinnati
2003-2022
Museum of Heilongjiang Province
2021
Trelleborg (Sweden)
2019
Office of Patient Care Services
2016
Cincinnati VA Medical Center
2007
The University of Texas MD Anderson Cancer Center
2000
The food-borne pathogen, Escherichia coli O157:H7, has been associated with gastrointestinal disease and the life-threatening sequela hemolytic uremic syndrome. genes for virulence factor, Shiga toxin 2 (Stx2), in E. O157:H7 are encoded on a temperate bacteriophage under regulation of late gene promoter. Induction phage lytic cycle is required synthesis release. We investigated hypothesis that nonpathogenic could amplify Stx2 production if infected toxin-encoding phage. Toxin-encoding were...
ABSTRACT Shiga toxin 2 (Stx2) from the foodborne pathogen Escherichia coli O157:H7 is encoded on a temperate bacteriophage. Toxin-encoding phages C600::933W and six clinical E. isolates were characterized for PCR polymorphisms, phage morphology, production, lytic lysogenic infection profiles O157 non-O157 serotype . The found to be highly variable, even isolated strains with identical pulsed-field gel electrophoresis differed. Examination of cross-plaquing lysogeny further substantiated that...
To assess the impact of coronavirus disease 2019 (COVID-19) pandemic on healthcare-associated infections (HAIs) reported from 128 acute-care and 132 long-term care Veterans Affairs (VA) facilities.We compared central-line-associated bloodstream (CLABSIs), ventilator-associated events (VAEs), catheter-associated urinary tract (CAUTIs), methicillin-resistant Staphylococcus aureus (MRSA), Clostridioides difficile rates each facility monthly to a centralized database before (February through...
The presence of commensal flora reduced colonization Escherichia coli O157:H7 and production Shiga toxin (Stx) in the murine intestine. Stx was not detected mice colonized with E. that were resistant to phage, but it phage-susceptible coli.
Choose your poison: Shiga toxins 1 and 2 are the major virulence factors of E. coli O157:H7. However, is more potent than 1. Biotinylated glycoconjugates have been developed that differentiate between these structurally homologous (see picture; R=OH: selective for 1; R=NHAc: 2). These synthetic materials efficiently capture without interference from sample matrix.
The AB(5) toxin Shiga 2 (Stx2) has been implicated as a major virulence factor of Escherichia coli O157:H7 and other toxin-producing E. strains in the progression intestinal disease to more severe systemic complications. Here, we demonstrate that supernatant from normal isolate, FI-29, neutralizes effect Stx2, but not related Stx1, on Vero cells. Biochemical characterization neutralizing activity identified lipopolysaccharide (LPS) serogroup O107/O117 strain, toxin-neutralizing component....
Legionella growth in healthcare building water systems can result legionellosis, making management programs (WMPs) important for patient safety. However, knowledge is limited on prevalence buildings. A dataset of quarterly testing Veterans Health Administration (VHA) buildings was used to examine national environmental from 2015 2018. Bayesian hierarchical logistic regression modeling assessed factors influencing positivity. The master included 201,146 samples 814 at 168 VHA campuses....
The conventional treatment of uterine leiomyomas, or fibroids, with gonadotropin-releasing hormone (GnRH) agonists is often associated serious side effects, necessitating short-term, palliative use this therapy. Therefore, we examined a retinoid X receptor (RXR)-selective ligand, LGD1069, as possible for leiomyoma. LGD1069 has demonstrated efficacy chemopreventive agent in the N-nitroso-N-methylurea (NMU)-induced rat mammary carcinoma model and therapeutic several epithelial tumor models....
Legionnaires disease (LD) incidence is increasing in the United States. Health care facilities are a high-risk setting for transmission of Legionella bacteria from building water systems to occupants. However, contribution LD health national rates not well characterized.To determine burden US Department Veterans Affairs (VA) patients and assess amount with VA exposure.Retrospective cohort study reported data medical surveillance system January 1, 2014, December 31, 2016. The population...
Background Patient travel history can be crucial in evaluating evolving infectious disease events. Such information challenging to acquire electronic health records, as it is often available only unstructured text. Objective This study aims assess the feasibility of annotating and automatically extracting mentions from clinical documents Department Veterans Affairs across disparate care facilities among millions patients. Information about exposure augments existing surveillance applications...
Zucker gegen Gifte: Die Shiga-Toxine 1 und 2 sind die wichtigsten Giftstoffe von E. coli O157:H7, wobei Shiga wirksamer ist als 1. Biotinylierte Glycokonjugate können zwischen den strukturhomologen Toxinen unterscheiden (siehe Formel; R = OH: Shiga-1-selektiv; NHAc: Shiga-2-selektiv). Diese synthetischen Substanzen fangen Toxine effizient ohne Störung durch Probenmatrix ein. Supporting information for this article is available on the WWW under...
The Veterans Health Administration requires implementation of Legionella prevention policy in potable water systems at Department Affairs (VA) medical facilities across the United States and territories.The Central Office program offices with expertise engineering clinical aspects have provided joint, structured on-site assistance to VA for consultation on implementation. Site visits included review facility documentation data, discussions staff, touring buildings, development...
Background and objectives Research on Legionnaires’ Disease (LD) suggests there may be long-term health complications, but data are limited. This study investigated whether Intensive Care Unit (ICU) admission during LD hospitalization associated with adverse outcomes characterized subsequent discharge diagnoses in patients up to 5 years post-LD. Methods We conducted a retrospective case series follow for among hospitalized at Department of Veterans Affairs (VA) Medical Center between 2005...
Abstract Clostridioides difficile infection rates from 7 facilities that used nucleic acid amplification testing (NAAT) alone for 12 months then switched to NAAT plus toxin enzyme immunoassay (EIA) and reported the latter result were compared 70 all 24 months. There was no significant difference in between facility groups over first ( P = .21, linear regression), but we detected a decrease changed NAAT+EIA < .0001).
Healthcare-associated Legionnaires' disease (HCA LD) causes significant morbidity and mortality, with varying guidance on prevention. We describe the evaluation of a case possible HCA LD note pitfalls relying solely an epidemiologic definition for association facility. Our detailed investigation led to identification new Legionella pneumophila serogroup 1 sequence type, confirmed healthcare helped build framework our ongoing preventive efforts. experience highlights role routine...