A5091342114- Plant Taxonomy and Phylogenetics
- Plant tissue culture and regeneration
- Plant and fungal interactions
- Chromosomal and Genetic Variations
- Plant Pathogens and Resistance
- Botanical Research and Chemistry
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Wheat and Barley Genetics and Pathology
- Botanical Research and Applications
- Seedling growth and survival studies
- Plant Genetic and Mutation Studies
- Plant Disease Resistance and Genetics
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Flowering Plant Growth and Cultivation
- Tree Root and Stability Studies
- Genetic and Environmental Crop Studies
- Genetic Mapping and Diversity in Plants and Animals
- Turfgrass Adaptation and Management
- Agricultural pest management studies
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Bioenergy crop production and management
- Potato Plant Research
Institute of Biosciences and Bioresources
2014-2024
National Research Council
2016-2024
Istituto di Genetica Molecolare
2006-2013
University of Perugia
2003
Piaggio Aerospace (Italy)
1992-2001
Apomixis is defined as clonal reproduction by seed. A comparative transcriptomic analysis was undertaken between apomictic and sexual genotypes of Paspalum simplex Morong to identify apomixis-related polymorphisms at the level mRNA. cDNA-AFLP (amplified fragment length polymorphism) profiling flowers several stages development yielded 202 amplicons that showed kinds expression specificities. Among these, large majority consisted were present only in specific flowers. Ten percent polymorphic...
Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. single locus which blocked terms of recombination. Partial sequence analysis the apomixis revealed structural features heterochromatin, namely presence repetitive elements, gene degeneration, and de-regulation. To test epigenetic control apomixis, study on distribution cytosine methylation at effect artificial DNA demethylation mode was undertaken two apomictic species. The 5-methylcytosine apomixis-controlling...
Apomixis in plants consists of asexual reproduction by seeds. Here we characterized at structural and functional levels an apomixis-linked sequence Paspalum simplex homologous to subunit 3 the ORIGIN RECOGNITION COMPLEX (ORC3). ORC is a multiprotein complex which controls DNA replication cell differentiation eukaryotes. Three PsORC3 copies were identified, each one specific expression profile. Of these, PsORC3a, for apomictic genotypes, pseudogene that was poorly constitutively expressed all...
Apomixis is a clonal mode of reproduction via seeds, which results from the failure meiosis and fertilization in sexual female reproductive pathway. In previous transcriptomic surveys, we identified mitogen-activated protein kinase (N46) displaying differential representation florets apomictic Paspalum notatum genotypes. Here retrieved characterized N46 full cDNA sequence floral transcriptomes. Phylogenetic analyses showed that was member YODA family, re-named QUI-GON JINN (QGJ)....
A mapping population of Paspalum simplex segregating for apomixis (asexual reproduction through seeds) was screened with AFLPs to find apomixis-linked markers. Four linked in coupling phase were found. Three them did not show recombinants among the 87 individuals population, whereas other more loosely linked. Integrating AFLP data those obtained previously rice RFLP anchor markers, a map drawn chromosome region P. encompassing apomixis. We cloned three tightly into plasmid vectors and used...
Abstract Paspalum notatum Flüggé is an economically important subtropical fodder grass that widely used in the Americas. Here, we report a new chromosome-scale genome assembly and annotation of diploid biotype collected center origin species. Using Oxford Nanopore long reads, generated 557.81 Mb (N50 = 56.1 Mb) with high gene completeness (BUSCO 98.73%). Genome identified 320 (57.86%) repetitive elements 45,074 models, which 36,079 have level confidence. Further characterisation included...
Apomictic tetraploid Paspalum simplex was crossed with colchicine-doubled diploid sexual plants belonging to the same species. Homologous genomic probes were selected from a partial PstI library for their capacity detect alleles specific apomictic parent, and segregation analyzed in F 1 progeny. High levels of polymorphism between genotypes recorded. The heterozygosity high both but differences them not as great expected. In some markers segregated either monoallelic duplex or diallelic...
Abstract Three seed lots, obtained from different ‘foundation farms’, for each of the alfalfa, Medicago sativa L., ecotypes ‘Vogherese’ and ‘Maremmana’, together with variety ‘Iside’ as a control, were studied to test possibility distinguishing them through restriction fragment length polymorphism analysis. Twenty plants per lot analysed 25 DNA probes that yielded 155 informative polymorphic fragments. Variance partitioning showed within‐population variability accounted nearly 98% total...
Abstract The introgression of apomixis in major seed crops, would guarantee self-seeding superior heterotic seeds over generations. In the grass species Paspalum simplex , is controlled by a single locus which recombination blocked. perspective isolating genetic determinants apomixis, we report data on sequencing, silico mapping and expression analysis some genes contained two cloned genomic regions P . allowed us to identify conserved synteny group homoeologous locus, located telomeric...
Hypericum perforatum L. (2n=4x=32) is an attractive model system for the study of aposporous apomixis. The earliest phenotypic features apomixis in this species are mitotic formation unreduced embryo sacs from a somatic cell ovule nucellus and avoidance meiosis. In research we addressed gene expression variation sexual apomictic plants, by focusing on nucellus, which cellular domain primarily involved into differentiation meiocyte precursors sacs, at pre-meiotic developmental stage. Gene...