A5040344297- Multiple Sclerosis Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Barrier Structure and Function Studies
- Cancer-related molecular mechanisms research
Yale University
2018-2021
Abstract Background Inflammation in chronic active lesions occurs behind a closed blood–brain barrier and cannot be detected with MRI. Activated microglia are highly enriched for iron can visualized quantitative susceptibility mapping (QSM), an MRI technique used to delineate iron. Objective To characterize the histopathological correlates of different QSM hyperintensity patterns MS lesions. Methods brain slabs were imaged QSM, processed histology. Immunolabeled cells quantified lesion rim,...
Epigenetic annotation studies of genetic risk variants for multiple sclerosis (MS) implicate dysfunctional lymphocytes in MS susceptibility; however, the role central nervous system (CNS) cells remains unclear. We investigated effect variant, rs7665090G, located near NFKB1, on astrocytes. demonstrated that chromatin is accessible at locus, a prerequisite its impact astroglial function. The variant was associated with increased NF-κB signaling and target gene expression, driving lymphocyte...
Activated myeloid cells and astrocytes are the predominant cell types in active multiple sclerosis (MS) lesions. Both can adopt diverse functional states that play critical roles lesion formation resolution. In order to identify phenotypic subsets of astrocytes, we profiled two MS lesions with thirteen glial activation markers using imaging mass cytometry (IMC), a method for multiplexed labeling histological sections. acutely demyelinating lesion, found distinct astrocyte phenotypes...
Abstract β cells may participate and contribute to their own demise during Type 1 diabetes (T1D). Here we report a role of expression Tet2 in regulating immune killing. is induced murine human with inflammation but its reduced surviving cells. Tet2-KO mice that receive WT bone marrow transplants develop insulitis not islet infiltrates do eliminate even though from the can transfer NOD/ scid recipients. recipients are protected disease by diabetogenic cells.Tet2-KO show IFNγ-induced...
Abstract Activated myeloid cells and astrocytes are the predominant cell types in active multiple sclerosis (MS) lesions. Both can adopt diverse functional states that play critical roles lesion formation resolution. In order to identify phenotypic subsets of astrocytes, we profiled acute MS lesions with thirteen glial activation markers using imaging mass cytometry (IMC), a method for multiplexed labeling histological sections. demyelinating lesion, found distinct astrocyte phenotypes...
Abstract β cells may participate and contribute to their own demise during Type 1 diabetes (T1D). We identified a novel role of Tet2 in regulating immune killing cells. is induced murine human with inflammation but its expression reduced surviving Tet2-KO mice that receive WT bone marrow transplants develop insulitis not islet infiltrates do eliminate even though from the can transfer NOD/ scid recipients. show inflammatory genes, associated closed transcription factor binding sites....