A5050704727- Multiple Sclerosis Research Studies
- Advanced Neuroimaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Ultrasound and Hyperthermia Applications
- Ultrasound Imaging and Elastography
- Functional Brain Connectivity Studies
- Peripheral Neuropathies and Disorders
- RNA regulation and disease
- Neurogenesis and neuroplasticity mechanisms
- Polyomavirus and related diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Herpesvirus Infections and Treatments
- Cerebrospinal fluid and hydrocephalus
- Radiomics and Machine Learning in Medical Imaging
- Cytokine Signaling Pathways and Interactions
- Gene expression and cancer classification
- Viral Infections and Immunology Research
- Systemic Lupus Erythematosus Research
- Rheumatoid Arthritis Research and Therapies
- Digital Imaging for Blood Diseases
- Medical Imaging Techniques and Applications
- Systemic Sclerosis and Related Diseases
- Image Processing Techniques and Applications
- Nuclear Receptors and Signaling
- Immunotherapy and Immune Responses
Weill Cornell Medicine
2016-2025
Cornell University
2016-2025
MIND Research Institute
2015-2024
Memorial Sloan Kettering Cancer Center
2022
University of Michigan
2022
Columbia University Irving Medical Center
2022
New York University
2022
Yale New Haven Hospital
2022
Yale University
2022
Nanyang Technological University
2022
In mice, natural resistance or susceptibility to infection with intracellular parasites is determined by a locus group of loci on chromosome 1, designated Bcg, Lsh, and Ity, which controls early microbial replication in reticuloendothelial organs. We have identified positional cloning candidate gene for Nramp1, codes novel macrophage-specific membrane transport protein. created mouse mutant bearing null allele at we analyzed the effect such mutation infection. Targeted disruption Nramp1 has...
Purpose To assess multiple sclerosis (MS) lesions at various ages by using quantitative susceptibility mapping (QSM) and conventional magnetic resonance (MR) imaging. Materials Methods Retrospectively selected were 32 clinically confirmed MS patients (nine men 23 women; 39.3 years ± 10.9) who underwent two MR examinations (interval, 0.43 0.16) with three-dimensional gradient-echo sequence from August 2011 to 2012. estimate the of lesions, performed 0.3–10.6 before study studied....
Purpose Within multiple sclerosis (MS) lesions iron is present in chronically activated microglia. Thus, detection with MRI might provide a biomarker for chronic inflammation within lesions. Here, we examine contributions of and myelin to magnetic susceptibility on quantitative mapping (QSM). Methods Fixed MS brain tissue was assessed including gradient echo data, which processed generate field (phase), R2* QSM. Five were sectioned evaluated by immunohistochemistry presence myelin,...
Chronic active multiple sclerosis lesions, characterized by a hyperintense rim of iron-enriched, activated microglia and macrophages, have been linked to greater tissue damage. Post-mortem studies determined that chronic lesions are primarily related the later stages sclerosis; however, occurrence these their relationship earlier disease may be greatly underestimated. Detection across patient spectrum requires validated imaging tool accurately identify with persistent inflammation....
The natural resistance associated macrophage protein (Nramp) gene family is composed of two members in mammals, Nramp1 and Nramp2. expressed primarily macrophages mutations at this locus cause susceptibility to infectious diseases. Nramp2 has a much broader range tissue expression result iron deficiency, indicating role for metabolism. To get further insight into the function mechanism action Nramp proteins, we have generated isoform specific anti-Nramp1 anti-Nramp2 antisera. Immunoblotting...
Abstract The mouse Nramp1 gene (Bcg/Ity/Lsh) controls innate defense to infection with intracellular parasites such as Mycobacterium, Salmonella, and Leishmania. Sequence analysis of predicts a hydrophobic, membrane-associated protein expressed exclusively in monocyte/macrophage lineages. A single G169D substitution within the fourth predicted transmembrane domain is associated susceptibility (Bcg) inbred strains. To initiate biochemical characterization analyze molecular basis Nramp1(D169)...
Neural tube defects (NTDs) are very frequent congenital abnormalities in humans. Recently, we have documented independent association of Vangl1 and Vangl2 gene mutations with NTDs. In the Looptail mouse, homozygosity (but not heterozygosity) for loss-of-function alleles at causes severe NTD craniorachischisis, whereas heterozygosity mutant variants VANGL1 is associated NTDs a human cohort sporadic familial cases. To understand role normal development, created mouse an inactivating mutation...
Purpose To assess the reproducibility of brain quantitative susceptibility mapping (QSM) in healthy subjects and patients with multiple sclerosis (MS) on 1.5 3T scanners from two vendors. Materials Methods Ten volunteers 10 were scanned twice a scanner one vendor. The also 1.5T same vendor second Similar imaging parameters used for all scans. QSM images reconstructed using recently developed nonlinear morphology‐enabled dipole inversion (MEDI) algorithm L1 regularization. Region‐of‐interest...
Purpose To measure the longitudinal change in multiple sclerosis (MS) lesion susceptibility using quantitative mapping (QSM). Materials and Methods The study was approved by our Institutional Review Board. Longitudinal changes values of new enhanced‐with‐Gd MS lesions were measured at baseline magnetic resonance imaging (MRI) on a follow‐up MRI 29 patients within 2 years 3D echo gradient sequence 3T scanner. Paired t ‐test generalized estimating equations (GEE) model used to analyze change....
Abstract Background Inflammation in chronic active lesions occurs behind a closed blood–brain barrier and cannot be detected with MRI. Activated microglia are highly enriched for iron can visualized quantitative susceptibility mapping (QSM), an MRI technique used to delineate iron. Objective To characterize the histopathological correlates of different QSM hyperintensity patterns MS lesions. Methods brain slabs were imaged QSM, processed histology. Immunolabeled cells quantified lesion rim,...
Abstract Although multiple sclerosis has traditionally been considered a white matter disease, extensive research documents the presence and importance of grey injury including cortical deep regions. The exhibits broad range pathology is uniquely suited to study mechanisms clinical relevance tissue in using magnetic resonance techniques. Deep associated with cognitive disability. Recently, MRI characterization properties, such as thalamic volume, have tested potential trial end points...
<h3>Background and Objectives</h3> To determine the effects of dimethyl fumarate (DMF) glatiramer acetate on iron content in chronic active lesions patients with multiple sclerosis (MS) human microglia vitro. <h3>Methods</h3> This was a retrospective observational study 34 relapsing-remitting MS clinically isolated syndrome treated DMF or acetate. Patients had hyperintense rims quantitative susceptibility mapping, were (GA), minimum 2 on-treatment scans. Changes rim compared among treatment...
Abstract Quantitative susceptibility mapping (QSM), an imaging technique sensitive to brain iron, has been used detect paramagnetic rims of iron-laden active microglia and macrophages in a subset multiple sclerosis (MS) lesions, known as rim+ that are consistent with chronic lesions. Because the potential impact lesions on disease progression tissue damage, investigating their influence disability neurodegeneration is critical establish these course. This study aimed explore relationship...
Multiple Sclerosis (MS) is a complex disease of the CNS thought to require an environmental trigger. Gut dysbiosis common in MS, but specifically causative species are unknown. To address this knowledge gap, we used sensitive and quantitative PCR detection show that people with MS were more likely harbor greater abundance epsilon toxin (ETX)-producing strains C. perfringens within their gut microbiomes compared healthy controls (HC). patient-derived isolates produced functional ETX had...
The murine Bcg/Ity/Lsh locus determines the susceptibilities of inbred strains to infection with unrelated intracellular parasites, such as Mycobacterium bovis, Salmonella typhimurium, and Leishmania donovani. A candidate for Bcg/Ity/Lsh, designated Nramp1, has been recently identified shown encode a novel integral membrane protein that is expressed exclusively in professional phagocytes but whose function remains unknown. In strains, susceptibility associated single glycine-to-aspartic acid...
To demonstrate the phase and quantitative susceptibility mapping (QSM) patterns created by solid shell spatial distributions of magnetic in multiple sclerosis (MS) lesions.Numerical simulations experimental phantoms solid- shell-shaped sources were used to generate magnitude, phase, QSM images. Imaging 20 consecutive MS patients was also reviewed for this Institutional Review Board (IRB)-approved MRI study identify appearance lesions on images.Solid correctly reconstructed images, while...
<h3>BACKGROUND AND PURPOSE:</h3> Quantitative susceptibility mapping and R2* are sensitive to myelin iron changes in multiple sclerosis lesions. This study was designed characterize lesion on quantitative at various gadolinium-enhancement stages. <h3>MATERIALS METHODS:</h3> included 64 patients with MS different enhancing patterns white matter lesions: nodular, shell-like, nonenhancing < 1 year old, 1–3 years old. These represent acute, late early chronic, chronic lesions, respectively....
Investigating the potential of myelin repair strategies in multiple sclerosis (MS) requires an understanding dynamics during lesion evolution. The objective this study is to longitudinally measure water fraction (MWF), MRI biomarker myelin, new MS lesions and identify factors that influence their subsequent content. Twenty-three patients were scanned with whole-brain Fast Acquisition Spiral Trajectory T2prep (FAST-T2) MWF mapping at baseline median follow-up 6 months. Eleven healthy controls...