A5060070870- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
- Allergic Rhinitis and Sensitization
- Psoriasis: Treatment and Pathogenesis
- Asthma and respiratory diseases
- Skin Protection and Aging
- Urticaria and Related Conditions
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Contact Dermatitis and Allergies
- Cytokine Signaling Pathways and Interactions
- Gut microbiota and health
- T-cell and B-cell Immunology
- Inflammasome and immune disorders
- Pharmacological Effects of Natural Compounds
- Estrogen and related hormone effects
- Food Allergy and Anaphylaxis Research
- 3D Printing in Biomedical Research
- Bacillus and Francisella bacterial research
- Wound Healing and Treatments
- Corneal Surgery and Treatments
- interferon and immune responses
- Mycobacterium research and diagnosis
- Hereditary Neurological Disorders
- Transgenic Plants and Applications
Radboud University Nijmegen
2015-2024
Radboud University Medical Center
2015-2024
Radboud Institute for Molecular Life Sciences
2016-2024
University Medical Center
2024
Previous studies have extensively documented antimicrobial and chemotactic activities of beta-defensins. Human beta-defensin-2 (hBD-2) is strongly expressed in lesional psoriatic epidermis, recently we shown that high beta-defensin genomic copy number associated with psoriasis susceptibility. It not known, however, if biologically pathophysiologically relevant concentrations hBD-2 protein are present vivo, which could support an proinflammatory role beta-defensins epidermis.We found systemic...
Journal Article Molecular diagnostics of psoriasis, atopic dermatitis, allergic contact dermatitis and irritant Get access M. Kamsteeg, Kamsteeg Department Dermatology, Search for other works by this author on: Oxford Academic Google Scholar P.A.M. Jansen, Jansen Dermatology,Nijmegen Centre Life Sciences, I.M.J.J. Van Vlijmen‐Willems, Vlijmen‐Willems P.E.J. Erp, Erp D. Rodijk‐Olthuis, Rodijk‐Olthuis P.G. Der Valk, Valk T. Feuth, Feuth Epidemiology, Biostatistics Health Technology Assessment,...
Terminally differentiating epidermal keratinocytes express a large number of structural and antimicrobial proteins that are involved in the physical barrier function stratum corneum provide innate cutaneous host defense. Late cornified envelope (LCE) genes, located differentiation complex on chromosome 1, encode family 18 unknown function, whose expression is largely restricted to epidermis. Deletion two members, LCE3B LCE3C (LCE3B/C-del), widely-replicated psoriasis risk factor interacts...
The effectiveness of biologics for psoriasis shows heterogeneity among patients. With pharmacogenetic markers, it might be possible to predict treatment response. We aimed test the association between genetic markers and response in (etanercept, adalimumab, ustekinumab) a prospective cohort. investigated copy number variation LCE3B LCE3C genes, eight single‐nucleotide polymorphisms (SNPs) HLA‐C*06, CD84, IL12B, IL23R, TRAF3IP2, ERAP1, IFIH1 TNFAIP3. decrease Psoriasis Area Severity Index...
Psoriasis and atopic dermatitis are chronic inflammatory skin diseases characterized by keratinocyte (KC) hyperproliferation epidermal acanthosis (hyperplasia). The milieu of disease-associated cytokines soluble factors is considered a mitogenic factor; however, pinpointing the exact mitogens in this complex microenvironment challenging. We employed organotypic human equivalents, faithfully mimicking native proliferation stratification, to evaluate proliferative effects broad panel...
In the past decades, chronic inflammatory diseases such as psoriasis, atopic dermatitis, asthma, Crohn's disease and celiac were generally regarded immune-mediated conditions involving activated T-cells proinflammatory cytokines produced by these cells. This paradigm has recently been challenged finding that mutations polymorphisms in epithelium-expressed genes involved physical barrier function or innate immunity, are risk factors of conditions. We used a functional genomics approach to...
The use of tissue-engineered human skin equivalents (HSE) for fundamental research and industrial application requires the expansion keratinocytes from a limited number biopsies donated by adult healthy volunteers or patients. A pharmacological inhibitor Rho-associated protein kinases, Y-27632, was recently reported to immortalize neonatal foreskin keratinocytes. Here, we investigated potential Y-27632 expand evaluated its effects on HSE development in vitro gene delivery assays. found...
Cystatin M/E (CST6) is a nonredundant, epithelium-specific protease inhibitor with presumed role in epidermal differentiation and tumor suppression. We have previously reported that cystatin deficiency Cst6−/− mice causes neonatal lethality because of excessive transepidermal water loss. Biochemical evidence suggests controls the activity legumain, cathepsin L, V, transglutaminase-3. Using genetic approach we sought to define epithelial biology by identification its target proteases their...
Late cornified envelope (LCE) proteins are small cationic epidermal with antimicrobial properties, and the combined deletion of LCE3B LCE3C genes is a risk factor for psoriasis that affects skin microbiome composition. In yeast two-hybrid screen, we identified CYSRT1 as an interacting partner members all LCE groups except LCE6. These interactions were confirmed in mammalian cell system by coimmunoprecipitation. protein unknown function specifically expressed cutaneous oral epithelia...
In atopic dermatitis (AD), chronic skin inflammation is associated with barrier defects and microbiome dysbiosis including a lower abundance of Gram-positive anaerobic cocci (GPACs). We here report that, through secreted soluble factors, GPAC rapidly directly induced epidermal host-defense molecules in cultured human keratinocytes indirectly via immune-cell activation cytokines derived thereof. Host-derived antimicrobial peptides known to limit the growth Staphylococcus aureus-a pathogen...
Following descriptive studies on skin microbiota in health and disease, mechanistic the interplay between microbes are rise, for which experimental models great demand. Here, we present a novel methodology microbial colonization of organotypic analysis thereof.An inoculation device ensured standardized application area stratum corneum homogenous distribution bacteria, while preventing infection basolateral culture medium even during prolonged periods up to 2 weeks at specific temperature...
The transmembrane protein claudin-1 is critical for formation of the epidermal barrier structure called tight junctions (TJ) and has been shown to be important in multiple disease states. These include neonatal ichthyosis sclerosing cholangitis syndrome, atopic dermatitis various viral infections. To develop a model investigate role different settings, we used CRISPR/Cas9 generate human immortalized keratinocyte (KC) lines lacking (CLDN1 KO). We then determined whether loss expression...