A5070844916- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Silk-based biomaterials and applications
- Silkworms and Sericulture Research
- Viral Infections and Immunology Research
- Immune responses and vaccinations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Herpesvirus Infections and Treatments
- Genetic Mapping and Diversity in Plants and Animals
- Plant Reproductive Biology
- Influenza Virus Research Studies
- Genomics and Chromatin Dynamics
Guangzhou Experimental Station
2023-2025
University of California, Irvine
2017-2023
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2022
Chinese Academy of Sciences
2012-2015
Center for Excellence in Molecular Plant Sciences
2012-2013
Shanghai Institutes for Biological Sciences
2012-2013
Albert Einstein College of Medicine
2013
Sericultural Research Institute
2009-2010
Chinese Academy of Agricultural Sciences
2009-2010
Abstract Infection by viruses, including herpes simplex virus-1 (HSV-1), and cellular stresses cause widespread disruption of transcription termination (DoTT) RNA polymerase II (RNAPII) in host genes. However, the underlying mechanisms remain unclear. Here, we demonstrate that HSV-1 immediate early protein ICP27 induces DoTT directly binding to essential mRNA 3’ processing factor CPSF. It thereby assembly a dead-end complex, blocking cleavage. Remarkably, also acts as sequence-dependent...
The persistent emergence of COVID-19 variants and recurrent waves infection worldwide underscores the urgent need for vaccines that effectively reduce viral transmission prevent infections. Current intramuscular (IM) inadequately protect upper respiratory mucosa. In response, we have developed a nonadjuvanted, interferon-armed SARS-CoV-2 fusion protein vaccine with IM priming intranasal (IN) boost sequential immunization. Our study showed this vaccination strategy IM+IN significantly...
Fidelity and efficiency of pre-mRNA splicing are critical for generating functional mRNAs, but how such accuracy in 5′ splice site (SS) selection is attained not fully clear. Through a series yeast genetic screens, we isolated alleles prp28 that improve suboptimal 5′SS substrates, demonstrating WT-Prp28p proofreads, consequently rejects, poor 5′SS. Prp28p thought to facilitate the disruption 5′SS–U1 snRNA pairing allow 5′SS–U6 catalytic spliceosome; unexpectedly, proofreading by dependent on...
Alternative splicing and trans-splicing events have not been systematically studied in the silkworm Bombyx mori. Here, transcriptome was analyzed by RNA-seq. We identified 320 novel genes, modified 1140 gene models, found thousands of alternative 58 events. Studies three SR proteins show that both their patterns mRNA products are conserved from insect to human, one isoform Srsf6 with a retained intron is expressed sex-specifically gonads. Trans-splicing mod(mdg4) experimentally confirmed....
Eukaryotic gene expression is extensively regulated by cellular stress and pathogen infections. We have previously shown that herpes simplex virus 1 (HSV-1) several stresses cause widespread disruption of transcription termination (DoTT) RNA polymerase II (RNAPII) in host genes the viral immediate early factor ICP27 plays an important role HSV-1-induced DoTT. Here, we show HSV-1 infection also leads to changes alternative polyadenylation (APA) mRNAs. In majority cases, shifts upstream...
Unlike typical cis-splicing, trans-splicing joins exons from two separate transcripts to produce chimeric mRNA and has been detected in most eukaryotes. Trans-splicing trypanosomes nematodes characterized as a spliced leader RNA-facilitated reaction; contrast, its mechanism higher eukaryotes remains unclear. Here we investigate mod(mdg4), classic trans-spliced gene Drosophila, report that critical RNA sequences the middle of last 5' intron, TSA TSB, promote mod(mdg4). In TSA, 13-nucleotide...
Abstract RNA molecules can fold into complex structures and interact with trans-acting factors to control their biology. Recent methods have been focused on developing novel tools measure structure transcriptome-wide, but utility study predict RNA-protein interactions or processing has limited thus far. Here, we extend these studies the first transcriptome-wide mapping method for cataloging solvent accessibility, icLASER. By combining accessibility (icLASER) flexibility (icSHAPE) data,...
JTE-607 is a small molecule compound with anti-inflammation and anti-cancer activities. Upon entering the cell, it hydrolyzed to Compound 2, which directly binds inhibits CPSF73, endonuclease for cleavage step in pre-mRNA 3' processing. Although CPSF73 universally required mRNA end formation, we have unexpectedly found that 2- mediated inhibition of processing sequence-specific sequences flanking site (CS) are major determinant drug sensitivity. By using massively parallel vitro assays,...
The non-lepis wing of silkworm (Bombyx mori) is controlled by the recessive gene, nlw. Owning to lack crossing over in females, reciprocal backcrossed F(1) (BC(1)) progenies were used for linkage analysis and mapping nlw based on SSR map STS markers using wild type (+(nlw)/+(nlw)) strain P50 U06 with scaleless (nlw/nlw). gene was linked eight one marker. All individuals BC1F (Using as female backcross parent, that (U06xP50)xU06) showed heterozygous profile (U06xP50) F(1), ones exhibited...
Abstract RNA molecules can fold into complex structures and interact with trans-acting factors to control their biology. Recent methods have been focused on developing novel tools measure structure transcriptome-wide, but utility study predict RNA-protein interactions or processing has limited thus far. Here, we extend these studies the first transcriptomewide mapping method for cataloging solvent accessibility, icLASER. By combining accessibility (icLASER) flexibility (icSHAPE) data,...
Abstract Eukaryotic gene expression is extensively regulated by cellular stress and pathogen infections. We have previously shown that herpes simplex virus 1 (HSV-1) several stresses cause widespread disruption of transcription termination (DoTT) RNA polymerase II (RNAPII) in host genes the viral immediate early factor ICP27 plays an important role HSV-1-induced DoTT. Here, we show HSV-1 infection also leads to changes alternative polyadenylation (APA) mRNAs. In majority cases, shifts...