A5055288729- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Viral Infections and Immunology Research
- Reproductive tract infections research
- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Hepatitis B Virus Studies
- interferon and immune responses
- Genital Health and Disease
- Parvovirus B19 Infection Studies
- Toxoplasma gondii Research Studies
- RNA regulation and disease
- HIV Research and Treatment
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- T-cell and Retrovirus Studies
- Polyomavirus and related diseases
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Long-Term Effects of COVID-19
University of Würzburg
2014-2024
University of Regensburg
2024
Riga Stradiņš University
2024
Institute of Life Sciences
2016
Molecular Oncology (United States)
2008
University of Allahabad
2008
DKFZ-ZMBH Alliance
2008
German Cancer Research Center
2008
Institute of Cytology and Preventive Oncology
2004-2007
Maulana Azad Medical College
2004-2005
The transcription factor AP-1 plays a central role in the transcriptional regulation of specific types high-risk human papillomaviruses (HPVs) such as HPV16 and HPV18, which are etiologically associated with development cancer uterine cervix women. In our study, we investigated binding activity expression pattern different members family (c-Jun, JunB, JunD, c-Fos, FosB, Fra-1 Fra-2) grades cervical lesions starting from mild dysplasia to invasive tumors, including normal control tissues,...
Obligate intracellular bacteria such as Chlamydia trachomatis depend on metabolites of the host cell and thus protect their sole replication niche by interfering with cells' stress response. Here, we investigated involvement microRNAs (miRNAs) in maintaining viability C. trachomatis-infected primary human cells. We identified miR-30c-5p a prominently up-regulated miRNA required for stable down-regulation p53, major suppressor metabolite supply Loss led to up-regulation Drp1, mitochondrial...
Abstract Infection by viruses, including herpes simplex virus-1 (HSV-1), and cellular stresses cause widespread disruption of transcription termination (DoTT) RNA polymerase II (RNAPII) in host genes. However, the underlying mechanisms remain unclear. Here, we demonstrate that HSV-1 immediate early protein ICP27 induces DoTT directly binding to essential mRNA 3’ processing factor CPSF. It thereby assembly a dead-end complex, blocking cleavage. Remarkably, also acts as sequence-dependent...
Abstract The predicted 80 open reading frames (ORFs) of herpes simplex virus 1 (HSV-1) have been intensively studied for decades. Here, we unravel the complete viral transcriptome and translatome during lytic infection with base-pair resolution by computational integration multi-omics data. We identify a total 201 transcripts 284 ORFs including all known 46 novel large ORFs. This includes so far unknown ORF in locus deleted FDA-approved oncolytic Imlygic. Multiple transcript isoforms...
ABSTRACT High-risk human papillomaviruses (HPVs), particularly HPV types 16 and 18 (HPV-16 HPV-18, respectively), play a cardinal role in the etiology of cervical cancer. The most prevalent type, HPV-16, shows intratypic sequence variants that are known to differ oncogenic potential geographic distribution. This study was designed analyze variations E6, E7, L1 genes LCR (for long control region) HPV-16 cancer patients identify novel correlate them with severity disease. Cervical biopsies...
Obligate intracellular bacteria depend entirely on nutrients from the host cell for their reproduction. Here, we show that obligate Chlamydia downregulate central tumor suppressor p53 in human cells. This reduction of levels is mediated by PI3K-Akt signaling pathway, activation HDM2, and subsequent proteasomal degradation p53. The stabilization cells severely impaired chlamydial development caused loss infectious particle formation. DNA-damage-induced interfered with through downregulation...
Obligate intracellular Chlamydia trachomatis replicate in a membrane-bound vacuole called inclusion, which serves as signaling interface with the host cell. Here, we show that chlamydial deubiquitinating enzyme (Cdu) 1 localizes inclusion membrane and faces cytosol active domain. The structure of this domain revealed high similarity to mammalian deubiquitinases unique α-helix close substrate-binding pocket. We identified apoptosis regulator Mcl-1 target interacts Cdu1 is stabilized by...
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a multifactorial disorder with many possible triggers. Human herpesvirus (HHV)-6 and HHV-7 are two infectious triggers for which evidence has been growing. To understand causative role of HHV-6 in ME/CFS, metabolic antiviral phenotypes U2-OS cells were studied without chromosomally integrated or virus reactivation using the histone deacetylase inhibitor trichostatin-A. Proteomic analysis was conducted by pulsed stable isotope...
Over the last decade, communication between immune cells by extracellular vesicle-associated miRNAs has emerged as an important regulator of coordinated response. Therefore, a thorough understanding conversation occurring via miRNAs, especially during infection, may provide novel insights into both host reaction to microbe well microbial
Both human herpes viruses and Chlamydia are highly prevalent in the population detected together different disorders. Here, we demonstrate that co-infection with virus 6 (HHV6) interferes developmental cycle of C. trachomatis induces persistence. Induction chlamydial persistence by HHV6 is independent productive infection, but requires interaction uptake host cell. On other hand, viral strongly promoted under conditions. Host cell glutathione reductase activity was suppressed causing NADPH...
More than 95% of the human population is infected with herpesvirus-6 (HHV-6) during early childhood and maintains latent HHV-6 genomes either in an extra-chromosomal form or as a chromosomally integrated (ciHHV-6). In addition, approximately 1% humans are born inheritable ciHHV-6 into telomeres chromosomes. Immunosuppression stress conditions can reactivate replication, which associated clinical complications even death. We have previously shown that Chlamydia trachomatis infection...
Abstract Acetaminophen (N-acetyl-para-aminophenol (APAP)) toxicity causes acute liver failure by inducing centrilobular hepatic damage as a consequence of mitochondrial oxidative stress. Sterile inflammation, triggered damage, facilitates gut bacterial translocation leading to systemic inflammation; TLR4-mediated activation LPS has been shown have critical role in APAP-mediated hepatotoxicity. In this study, we demonstrate significant protection mediated chitohexaose (Chtx) mice challenged...
Early-life infections and associated neuroinflammation is incriminated in the pathogenesis of various mood disorders. Infection with human roseoloviruses, HHV-6A HHV-6B, allows viral latency central nervous system other tissues, which can later be activated causing cognitive behavioral disturbances. Hence, this study was designed to evaluate possible association HHV-6B activation three different groups psychiatric patients. DNA qPCR, immunofluorescence FISH studies were carried out...
Herpes simplex virus 1 (HSV-1) induces a profound host shutoff during lytic infection. The virion (
Fulminant hepatitis in Asian pregnant women is generally caused by E virus infection, and extremely high mortality most common them. Decreased cell-mediated immunity considered a major cause of death these cases, but what exactly influences decreased specifically during pregnancy not known. We used electrophoretic mobility shift assays, immunoblotting, immunohistochemical analysis to study the expression DNA binding activity NF-kB p50 p65 fulminant hepatic failure (FHF) patients compared...
First exposure to various human herpesviruses (HHVs) including HHV-6, HCMV and EBV does not cause a life-threatening disease. In fact, most individuals are frequently unaware of their first such pathogens. These acquire lifelong latency in the body where they show minimal genomic activity required for survival. We hypothesized that it is itself but timely, regionally restricted viral reactivation sub-set host cells plays key role disease development. HHV-6 (HHV-6A HHV-6B) HHV-7 unique HHVs...
Human herpesvirus 7 (HHV-7) is a betaherpesvirus, and phylogenetically related to both HHV-6A HHV-6B. The presence of telomeric repeat sequences at ends its genome should make it equally likely integrate into the human telomere as HHV-6. However, numerous studies have failed detect germline integration HHV-7, suggesting an important difference between HHV-6A/-6B HHV-7 genomes. In search possible integrated we developed sensitive quantitative real-time PCR assay discovered that primers...
Specialized assembly factors facilitate the formation of many macromolecular complexes in vivo. The Sm core structures spliceosomal U-rich small nuclear ribonucleoprotein particles (UsnRNPs) requires united protein arginine methyltransferase 5 (PRMT5) and survival motor neuron (SMN) complexes. We demonstrate that perturbations this machinery trigger complex cellular responses prevent aggregation unassembled proteins. Inactivation SMN results initial tailback proteins on PRMT5 complex,...
Drug Reaction with Eosinophilia and Systemic Symptoms (DRESS) is a severe adverse event (mortality of 10%). Its pathophysiology involves herpesviruses, particularly HHV-6, but the exact mechanisms are still poorly understood.To describe cases DRESS especially their association herpesvirus reactivation.This study was multicentre case series conducted between 2007 2021 at five University Hospital Centres in France. The included patients who had DRESS, which defined as death, transfer to...