A5036762125- Cancer Cells and Metastasis
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- Esophageal Cancer Research and Treatment
- Cytomegalovirus and herpesvirus research
- Bat Biology and Ecology Studies
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Immune responses and vaccinations
- Viral Infections and Vectors
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Machine Learning in Bioinformatics
- T-cell and B-cell Immunology
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
Helmholtz Institute for RNA-based Infection Research
2021-2024
Biotechnologie et Signalisation Cellulaire
2020
Université de Strasbourg
2020
Centre National de la Recherche Scientifique
2020
Bats harbor high-impact zoonotic viruses often in the absence of disease manifestation. This restriction and tolerance possibly rely on specific immunological features. In-depth molecular characterization cellular immunity imprinting age leukocyte compartments remained unexplored bats. We employ single-cell RNA sequencing (scRNA-seq) establish immunostaining panels to characterize immune cell landscape juvenile, subadult, adult Egyptian rousette bats (ERBs). Transcriptomic flow cytometry...
Abstract The gastroesophageal squamocolumnar junction (GE-SCJ) is a critical tissue interface between the esophagus and stomach, with significant relevance in pathophysiology of gastrointestinal diseases. Despite this, molecular mechanisms underlying GE-SCJ development remain unclear. Using single-cell transcriptomics, organoids, spatial analysis, we examine cellular heterogeneity spatiotemporal dynamics from embryonic to adult mice. We identify distinct transcriptional states signaling...
Transcription factors (TFs) regulate the expression of gene expression. The binding specificities many TFs have been deciphered and summarized as position-weight matrices, also called TF motifs. Despite availability hundreds known motifs in databases, it remains non-trivial to quickly query visualize enrichment genomic regions interest. Towards this goal, we developed TFmotifView, a web server that allows study distribution regions. Based on input selected motifs, TFmotifView performs an...
Abstract The gastroesophageal junction (GEJ), where squamous and columnar epithelia meet, is a hotspot for Barrett’s metaplasia development, dysbiosis carcinogenesis. However, the mechanisms regulating GEJ homeostasis remain unclear. Here, by employing organoids, bulk single-cell transcriptomics, single-molecule RNA in situ hybridisations lineage tracing, we identified spatial organisation of epithelial, stromal compartment regulators that maintain normal homeostasis. During common KRT8...
Base editors create precise genomic edits by directing nucleobase deamination or removal without inducing double-stranded DNA breaks. However, a vast chemical space of other modifications remains to be explored for genome editing. Here, we harness the bacterial anti-phage toxin DarT2 append ADP-ribosyl moieties DNA, unlocking distinct editing outcomes in bacteria versus eukaryotes. Fusing an attenuated Cas9 nickase, program site-specific ADP-ribosylation thymines within target sequence. In...
Abstract CD19 CAR T cells and CD20 targeting cell engaging bispecific antibodies have been approved in B-cell Non-Hodgkin lymphoma lately, heralding a new clinical setting where patients are treated with both approaches, sequentially. The aim of our study was to investigate the selective pressure directed therapy on clonal architecture lymphoma. Using broad analytical pipeline, we identified truncating mutations gene encoding conferring antigen loss 80% relapsing from bispecs. Pronounced...