A5026804439- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Bat Biology and Ecology Studies
- Rabies epidemiology and control
- Animal Virus Infections Studies
- Virology and Viral Diseases
- T-cell and Retrovirus Studies
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- COVID-19 Clinical Research Studies
- Aquaculture disease management and microbiota
- Viral Infectious Diseases and Gene Expression in Insects
- Orthopedic Infections and Treatments
- Immune responses and vaccinations
- Genetic and phenotypic traits in livestock
- Thermoregulation and physiological responses
- Viral Infections and Outbreaks Research
- Erythrocyte Function and Pathophysiology
- Viral Infections and Immunology Research
- RNA Research and Splicing
Friedrich-Loeffler-Institut
2020-2024
Riemser Pharma (Germany)
2021
Raccoon dogs might have been intermediate hosts for severe acute respiratory syndrome-associated coronavirus in 2002-2004. We demonstrated susceptibility of raccoon to syndrome 2 infection and transmission in-contact animals. Infected animals had no signs illness. Virus replication tissue lesions occurred the nasal conchae.
Bats harbor high-impact zoonotic viruses often in the absence of disease manifestation. This restriction and tolerance possibly rely on specific immunological features. In-depth molecular characterization cellular immunity imprinting age leukocyte compartments remained unexplored bats. We employ single-cell RNA sequencing (scRNA-seq) establish immunostaining panels to characterize immune cell landscape juvenile, subadult, adult Egyptian rousette bats (ERBs). Transcriptomic flow cytometry...
Applied research is crucial in pushing the boundaries and finding a solution to age-old problem of dog-mediated rabies. Although oral vaccination dogs considered have great potential mass dog campaigns could far-reaching benefits, it perhaps most ignored all available tools efforts eliminate rabies, not least because limited data on immunogenicity, efficacy, safety rabies vaccine candidates. In this study, long-term immunogenicity local Thai after administration highly attenuated 3rd...
Hibernation is a widespread and highly efficient mechanism to save energy in mammals. However, one major challenge of hibernation maintaining blood circulation at low body temperatures, which strongly depends on the viscoelastic properties red cells (RBCs). Here, we examined physiologically relevant timescales thermomechanical hundreds thousands individual RBCs from hibernating common noctule bat ( Nyctalus noctula ), nonhibernating Egyptian fruit Rousettus aegyptiacus humans Homo sapiens )....
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China at the end of 2019, and became pandemic. The zoonotic virus most likely originated from bats, but definite intermediate hosts have not yet been identified. Raccoon dogs ( Nyctereutes procyonoides ) are kept for fur production, particular China, were suspected as potential host both SARS-CoV6 SARS-CoV2. Here we demonstrate susceptibility raccoon SARS-CoV-2 infection after intranasal inoculation transmission...
The rapid spread of the African swine fever virus (ASFV), causing severe disease with often high fatality rates in Eurasian suids, prevails as a threat for pig populations and dependent industries worldwide. Although advancing scientific progress continually enhances our understanding ASFV pathogenesis, alternative transmission routes have yet to be assessed. Here, we demonstrate that can efficiently transferred from infected boars naïve recipient gilts through artificial insemination (AI)....
The rapid spread of African swine fever virus (ASFV), causing severe and often lethal disease in domestic pigs Eurasian wild boar, continues to be a threat for pig populations dependent industries. Despite scientific achievements that deepen our understanding ASFV pathogenesis, alternative transmission routes remain elucidated. We previously demonstrated the efficient from infected boars naïve recipient gilts via artificial insemination, thereby highlighting importance surveillance boar...
Abstract In the European Union, African swine fever (ASF) affects wild boar ( Sus scrofa ) populations in several Member States. Knowledge of population connectivity is important for implementation control measures, particular establishment effective barriers. Population genetic comparisons neighbouring can be very helpful this respect. The present study investigated differentiation eastern Germany. This region has been affected by ASF since September 2020. A total 1,262 boars from 31...
Rapid spread of African swine fever virus (ASFV), causing severe disease with often high fatality rates in Eurasian suids, prevails as a threat for pig populations and dependent industries world-wide. Although advancing scientific progress continually enhances our understanding ASFV pathogenesis, alternative transmission routes have yet to be assessed. Here, we demonstrate that can efficiently transferred from infected boars naïve recipient gilts through artificial insemination...
The live genetically-engineered oral rabies virus (RABV) variant SPBN GASGAS induces long-lasting immunity in foxes and protection against challenge with an otherwise lethal dose of RABV field strains both after experimental parenteral routes administration. Induction RABV-specific binding antibodies immunoglobulin isotypes (IgM, total IgG, IgG1, IgG2) were comparable orally parenterally vaccinated foxes. Differences only observed the induction virus-neutralizing (VNA) titers, which...
Abstract Bats are the only mammals capable of powered flight and their body temperature can reach up to 42 °C during flight. Additionally, bats display robust type I IFN interferon (IFN-I) responses some species constitutively express IFN-α. Reference genes with stable expression under oscillations IFN-I release therefore critical for normalization quantitative reverse-transcription polymerase chain reaction (qRT-PCR) data in bats. The stability reference Rousettus aegyptiacus remains...
African swine fever (ASF) is a severe, globally important disease in domestic and wild pigs. The testing of alternative transmission routes has proven that the ASF virus (ASFV) can be efficiently transmitted to sows via semen from infected boars through artificial insemination. Boars intramuscularly inoculated with ASFV strain “Estonia 2014” showed grossly microscopically visible changes testis, epididymis, prostate, vesicular gland. gross lesions included hemorrhages on scrotum, testicular...
The discovery of bats as reservoir hosts for a number highly pathogenic zoonotic agents has led to an increasing interest infectious disease research in experimental studies with bats. Therefore, we established breeding colonies Rousettus aegyptiacus and Eidolon helvum fruit bats, which both have been identified relevant agents, such Marburg virus Lagos bat virus. Since 2013, individuals species recruited the Friedrich-Loeffler-Institut (FLI) from zoological gardens Europe, where these had...
The rapid spread of African swine fever virus (ASFV), causing severe and often lethal disease in domestic pigs Eurasian wild boar, continues to be a threat pig populations dependent industries. Despite scientific achievements that have deepened our understanding ASFV pathogenesis, alternative transmission routes for remain elucidated. We previously demonstrated the efficient from infected boars naïve recipient gilts via artificial insemination, thereby highlighting importance surveillance...
African swine fever (ASF) continues to spread and persist in the Eurasian wild boar population. The infection pressure resulting from infected carcasses environment can be a major contributor disease persistence spread. For this reason, it is crucial find safe efficient method of carcass disposal under different circumstances. In presented study, we investigated open-air composting winter conditions northeastern Europe, i.e., Lithuania. We demonstrate that ASF virus (ASFV) inactivated both...
Abstract Bats are the only mammals capable of powered flight and their body temperature can reach up to 42°C during flight. Additionally, bats display robust type I IFN interferon (IFN-I) responses some species constitutively express IFN-α. Reference genes with stable expression under oscillations IFN-I release therefore critical for normalization quantitative reverse-transcription polymerase chain reaction (qRT-PCR) data in bats. The stability reference Rousettus aegyptiacus remains...