A5011795003- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cardiovascular Issues in Pregnancy
- Cardiovascular Function and Risk Factors
- Aquaculture disease management and microbiota
- Cancer-related Molecular Pathways
- Pulmonary Hypertension Research and Treatments
- Neurogenetic and Muscular Disorders Research
- Molecular Biology Techniques and Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Genomics and Phylogenetic Studies
- Amyotrophic Lateral Sclerosis Research
- GDF15 and Related Biomarkers
- Chromosomal and Genetic Variations
- Genetic diversity and population structure
- Cell death mechanisms and regulation
- Cardiovascular Effects of Exercise
University of Colorado Boulder
2010-2016
Mutations in the RNA-binding protein FUS (fused sarcoma)/TLS have been shown to cause neurodegenerative disease amyotrophic lateral sclerosis (ALS), but normal role of is incompletely understood. We found that binds C-terminal domain (CTD) RNA polymerase II (RNAP2) and prevents inappropriate hyperphosphorylation Ser2 RNAP2 CTD at thousands human genes. The loss leads accumulation transcription start site a shift mRNA isoform expression toward early polyadenylation sites. Thus, addition its...
Background Although the hypertrophic responses of heart to pregnancy and exercise are both considered be physiological processes, they occur in quite different hormonal temporal settings. In this study, we have compared global transcriptional profiles left ventricular tissues at various time points during progression hypertrophy pregnancy. Methodology/Principal Findings The following groups female mice were analyzed: non-pregnant diestrus cycle sedentary control, mid-pregnant, late-pregnant,...
The infrequently feeding Burmese python ( Python molurus ) experiences significant and rapid postprandial cardiac hypertrophy followed by regression as digestion is completed. To begin to explore the molecular mechanisms of this response, we have sequenced assembled fasted postfed heart transcriptomes with Illumina technology using chicken Gallus gallus genome a reference. In addition, used RNA-seq analysis identify differences in expression biological processes signaling pathways between...
Tumor suppressor p53 plays a key role in DNA damage responses metazoa, yet more than half of human tumors show deficiencies. Therefore, understanding how therapeutic genotoxins such as ionizing radiation (IR) can elicit p53-independent manner is clinical importance. Drosophila has been good model to study the effects IR because well underlying genes are conserved this model, and streamlined gene families make loss-of-function analyses feasible. Indeed, only genetically tractable for...
The large subunit of the U2 auxiliary factor (U2AF) recognizes polypyrimidine tract (Py-tract) located adjacent to 3' splice site facilitate snRNP recruitment. While U2AF is considered essential for pre-mRNA splicing, its requirement splicing on a genome-wide level has not been analyzed. Using Solexa sequencing, we performed mRNA profiling in Schizosaccharomyces pombe U2AF(59) (prp2.1) temperature-sensitive mutant. Surprisingly, our analysis revealed that introns show range defects mutant...
The Drosophila polypyrimidine tract-binding protein (dmPTB or hephaestus) plays an important role during embryogenesis. A loss of function mutation, heph03429, results in varied defects embryonic developmental processes, leading to lethality. However, the suite molecular functions that are disrupted mutant remains unknown. We have used unbiased high throughput sequencing approach identify transcripts misregulated this mutant. Misregulated show evidence significantly altered patterns splicing...
The Drosophila polypyrimidine tract-binding protein (dmPTB or hephaestus) plays an important role during spermatogenesis. heph2 mutation in this gene results a specific defect spermatogenesis, causing aberrant spermatid individualization and male sterility. However, the array of molecular defects mutant remains uncharacterized. Using unbiased high throughput sequencing approach, we have identified transcripts that are misregulated mutant. Aberrant show altered expression levels, exon...
Pregnancy is associated with a prolonged cardiac volume overload that results in hypertrophy. In this respect, pregnancy-induced hypertrophy somewhat analogous to obtained by endurance exercise training. However, unlike training, pregnancy also accompanied significant changes the hormonal milieu and continuous as opposed intermittent. For reason, two settings are distinct. Very little known about molecular mechanisms mediate during pregnancy. The objective of study was determine...