A5058679265- Advanced Proteomics Techniques and Applications
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Lipid Membrane Structure and Behavior
Tokyo Medical and Dental University
2022
Otsuka (Japan)
2021
Ochanomizu University
2021
Abstract Phosphoinositides are a family of membrane lipids essential for many biological and pathological processes. Due to the existence multiple phosphoinositide regioisomers their low intracellular concentrations, profiling these linking specific acyl variant change in state have been difficult. To enable comprehensive analysis phosphorylation status chain identity, we develop PRMC-MS (Phosphoinositide Regioisomer Measurement by Chiral column chromatography Mass Spectrometry). Using this...
Abstract Cardiolipin (CL) localizes to curved membranes such as cristae in mitochondria well cell poles and division sites rod-shaped bacteria. CL is believed stabilize the membrane curvature by localizing of negative curvature. However, this hypothesis has not been tested because a lack appropriate tools distinguish inside outside lipid bilayers. In study, we provided first evidence that localized regions Escherichia coli using novel probe erylysin A-EGFP (EryA-EGFP). Staining E.coli...