A5046132781- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- Cell Adhesion Molecules Research
- Hippo pathway signaling and YAP/TAZ
- Heat Transfer and Optimization
- Axon Guidance and Neuronal Signaling
- Protein Tyrosine Phosphatases
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Combustion and flame dynamics
- Eosinophilic Disorders and Syndromes
- Neurological disorders and treatments
- Phase Change Materials Research
- Eosinophilic Esophagitis
- Calcium signaling and nucleotide metabolism
- Spacecraft and Cryogenic Technologies
- Coal Combustion and Slurry Processing
- Wnt/β-catenin signaling in development and cancer
- PI3K/AKT/mTOR signaling in cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Metabolism, Diabetes, and Cancer
- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
Shimizu (Japan)
2025
Akita University
2009-2022
Japan Science and Technology Agency
2022
Morpho (United States)
2017
Gunma University
2010-2014
Austrian Academy of Sciences
2009-2014
University of Tsukuba
2005-2011
Institute of Molecular Biotechnology
2009
Tokyo Metropolitan Institute of Medical Science
2000-2006
National Institute of Advanced Industrial Science and Technology
2001-2004
Abstract Inositol polyphosphate 4-phosphatase B (INPP4B) has been identified as a tumor suppressor mutated in human breast, ovary, and prostate cancers. The molecular mechanism underlying INPP4B's tumor-suppressive role is currently unknown. Here, we demonstrate that INPP4B restrains development by dephosphorylating the PtdIns(3,4,5)P3 accumulates situations of PTEN deficiency. In vitro, directly dephosphorylates PtdIns(3,4,5)P3. vivo, neither inactivation Inpp4b (Inpp4bΔ/Δ) nor heterozygous...
Abstract Phosphoinositides are a family of membrane lipids essential for many biological and pathological processes. Due to the existence multiple phosphoinositide regioisomers their low intracellular concentrations, profiling these linking specific acyl variant change in state have been difficult. To enable comprehensive analysis phosphorylation status chain identity, we develop PRMC-MS (Phosphoinositide Regioisomer Measurement by Chiral column chromatography Mass Spectrometry). Using this...
Mammalian phosphatidylcholine-specific phospholipase D1 (PLD1) is a signal transduction-activated enzyme thought to function in multiple cell biological settings including the regulation of membrane vesicular trafficking. PLD1 activated by small G proteins, ADP-ribosylation factor (ARF) and RhoA, protein kinase C-alpha (PKC-alpha). This stimulation has been proposed involve direct interaction take place at distinct site for each activator. In present study, we employed yeast two-hybrid...
In Drosophila, planar cell polarity (PCP) molecules such as Dachsous (Ds) may function global directional cues directing the asymmetrical localization of PCP core proteins Frizzled (Fz). However, relationship between Ds asymmetry and Fz in eye is opposite to that wing, thereby causing controversy regarding how these two systems are connected. Here, we show this determined by ratio Prickle (Pk) isoforms, Pk Spiny-legs (Sple). Sple form different complexes with distinct subcellular...
The metabolism of membrane phosphoinositides is critical for a variety cellular processes. Phosphatidylinositol-3,5-bisphosphate [PtdIns(3,5)P 2 ] controls multiple steps the intracellular trafficking system in both yeast and mammalian cells. However, other than neuronal tissues, little known about physiological functions PtdIns(3,5)P mammals. Here, we provide genetic evidence that type III phosphatidylinositol phosphate kinase (PIPKIII), which produces , essential polarized epithelial...
The equivalent complex permittivity of walls, floors, and ceilings (composite building materials) in a was estimated by the free-space method for 28 GHz, frequency used 5G. R2coefficients 0.85〜0.93 were obtained comparing simulation results ray tracing with radio wave intensity real space, it confirmed that reproduce actual situation very well. Finally, spatial distribution office space simulated, which showed using is useful designing environments buildings.
Eosinophils and their secretory proteins are important in necroinflammatory processes. In this study we surveyed eosinophilic infiltration of the liver 176 biopsy specimens from patients with primary biliary cirrhosis. A considerable number eosinophils were found portal tracts 51 these (29%), whereas finding occurred only 2 50 (4%) viral chronic active hepatitis. Eosinophilic was focal. These immunohistochemically shown to contain secreted form cationic protein. Grades higher those livers...
Phosphatidylinositol 4-phosphate 5-kinase (PIP-5kin) regulates actin cytoskeletal reorganization through its product phosphatidylinositol 4,5-bisphosphate. In the present study we demonstrate that PIP-5kin is essential for neurite remodeling, which regulated by in neuroblastoma N1E-115 cells. Overexpression of wild-type mouse PIP-5kin-alpha inhibits formation normally stimulated serum depletion, whereas a lipid kinase-defective mutant PIP-5kin-alpha, D266A, triggers extension even presence...
The membrane phospholipid phosphatidylinositol 4, 5-bisphosphate [PI(4,5)P2] is a critical signal transducer in eukaryotic cells. However, the physiological roles of type I phosphate kinases (PIPKIs) that synthesize PI(4,5)P2 are largely unknown. Here, we show α isozyme PIPKI (PIPKIα) negatively regulates mast cell functions and anaphylactic responses. In vitro, PIPKIα-deficient cells exhibited increased degranulation cytokine production after Fcε receptor-I cross-linking. vivo, PIPKIα−/−...
Stimulation of the neuronal cell adhesion molecule L1 in cerebellar granule neurons (CGNs) enhances neurite outgrowth and this response is inhibited by primary alcohol ethanol. Because alcohols suppress formation signaling lipid phosphatidic acid (PA) phospholipase D (PLD), observation prompted us to investigate whether PLD plays a role L1-mediated CGNs. In cerebellum postnatal day 8 mice, PLD2 protein was abundantly expressed, while PLD1 expression not detected. The L1-stimulated...
Actin cytoskeletal reorganization and membrane trafficking are important for spine morphogenesis. Here we investigated whether the small GTPase, ADP‐ribosylation factor 6 (ARF6), which regulates actin dynamics peripheral vesicular trafficking, is involved in regulation of formation. The developmental expression pattern ARF6 mouse hippocampus was similar to that post‐synaptic density protein‐95, these molecules colocalized hippocampal neurons. Overexpression a constitutively active mutant...
The signaling pathway that triggers morphological differentiation of PC12 cells is mediated by extracellular signal-regulated kinase (ERK), the classic mitogen-activated protein (MAP) kinase. However, mediators downstream ERK have not been identified. We show here phospholipase D2 (PLD2), which generates pleiotropic lipid phosphatidic acid (PA), links activation to neurite outgrowth in nerve growth factor (NGF)-stimulated cells. Increased expression wild type PLD2 (WT-PLD2) dramatically...
Hypertrophic cardiomyopathy (HCM) is a common heart disease with prevalence of 1 in 500 the general population. Several mutations genes encoding cardiac proteins have been found HCM patients, but these changes do not predict occurrence or prognosis and molecular mechanisms underlying remain largely elusive. Here we show that expression vacuolar protein sorting 34 (Vps34) reduced subset patients. In mouse model, muscle-specific loss Vps34 led to HCM-like manifestations sudden death....
The temperature dependence of thermophysical properties was evaluated for disodium hydrogenphosphate dodecahydrate, which is used in long-term, supercooled thermal energy storage (Super-TES). Super-TES stores at temperatures lower than the melting point phase-change material, reduces heat loss from system. Although degree supercooling depends not only on intensive material properties, such as temperatures, but also extensive total volume, except distilled water and metals there insufficient...
Stimulation of the epidermal growth factor receptor (EGFR) produces membrane ruffles through small G protein Rac1; however, signaling pathway from EGFR to Rac1 has not yet been clarified. Here, we show that autophosphorylation at tyrosine 992 is required for EGF‐induced ruffle formation in CHO cells. Signaling autophosphorylated appears be mediated by phospholipase C (PLC) γ1. Furthermore, activation EGF inhibited a PLC inhibitor. These results, taken together, suggest and subsequent PLCγ1...