A5083994840- Gastric Cancer Management and Outcomes
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Metastasis and carcinoma case studies
- Gastrointestinal Tumor Research and Treatment
- Pancreatic function and diabetes
- Microtubule and mitosis dynamics
- Gastrointestinal disorders and treatments
- Mitochondrial Function and Pathology
- Protist diversity and phylogeny
- Abdominal Trauma and Injuries
- Prenatal Screening and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Geomagnetism and Paleomagnetism Studies
- Genetic and Kidney Cyst Diseases
- Genetic factors in colorectal cancer
- Renal Diseases and Glomerulopathies
- Gallbladder and Bile Duct Disorders
- Photosynthetic Processes and Mechanisms
- Phagocytosis and Immune Regulation
- Pregnancy and preeclampsia studies
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Thyroid Cancer Diagnosis and Treatment
- Pharmacogenetics and Drug Metabolism
Niigata University of Pharmacy and Medical and Life Sciences
2022
Doshisha Women's College of Liberal Arts
2007-2020
Pharmaceutical Biotechnology (Czechia)
2012
Kureha (Japan)
2012
European Federation of Pharmaceutical Industries and Associations
2008
Kumamoto University
2006
The University of Tokyo
2003-2005
Honda (Japan)
2001
Nagano Red Cross Hospital
1995-1998
Ebina General Hospital
1995
The nascent phagosome progressively establishes an acidic milieu by acquiring a proton pump, the vacuolar-type ATPase (V-ATPase). However, origin of phagosomal V-ATPase remains poorly understood. We found that phagosomes were enriched with a3 subunit, which also accumulated in late endosomes and lysosomes. modified mouse Tcirg1 locus encoding subunit a3, to express a3-GFP fusion protein. Live-cell imaging immunofluorescence microscopy revealed received from tubular structures extending...
The metabolism of membrane phosphoinositides is critical for a variety cellular processes. Phosphatidylinositol-3,5-bisphosphate [PtdIns(3,5)P 2 ] controls multiple steps the intracellular trafficking system in both yeast and mammalian cells. However, other than neuronal tissues, little known about physiological functions PtdIns(3,5)P mammals. Here, we provide genetic evidence that type III phosphatidylinositol phosphate kinase (PIPKIII), which produces , essential polarized epithelial...
Endocytosis has been proposed to modulate cell signaling activities. However, the role of endocytosis in embryogenesis, which requires coordination multiple inputs, remained less understood. We previously showed that mouse embryos lacking a small guanosine triphosphate (GTP)-binding protein Rab7 implicated endocytic flow are defective gastrulation. Here, we investigate how subcellular defects associated with deficiency related observed developmental defects. Rab7-deficient fail organize...
Transcription elongation factor S-II/TFIIS promotes readthrough of transcriptional blocks by stimulating nascent RNA cleavage activity polymerase II in vitro. The biologic significance S-II function higher eukaryotes, however, remains unclear. To determine its role mammalian development, we generated S-II-deficient mice through targeted gene disruption. Homozygous null mutants died at midgestation with marked pallor, suggesting severe anemia. S-II(-/-) embryos had a decreased number...
Abstract Background: During transcription elongation, RNA polymerase II is arrested on the template when incorrect ribonucleotides are incorporated into nascent transcripts. Transcription factor S‐II enhances excision of these mis‐incorporated nucleotides by and stimulates elongation in vitro . This mechanism considered to be transcriptional proof‐reading, but its physiological relevance remains unknown. Results: We report that contributes maintenance fidelity vivo employed a genetic...
During early embryogenesis, before the conceptus forms placenta, maternal nutrients as well signaling molecules must reach embryo proper through a tightly sealed epithelial tissue, visceral endoderm (VE). The VE serves center for where exocytic and endocytic processes integrate signal production, perception termination. However, process in this important tissue has not been characterized. We show that delivery to lysosomes occurs via RAB7-dependent microautophagy. This is essential mammalian...
Background Vacuolar-type proton transporting ATPase (V-ATPase) is involved in the proper development of visual function. Mutations Tcirg1 (also known as Atp6V0a3) locus, which encodes a3 subunit V-ATPase, cause severe autosomal recessive osteopetrosis (ARO) humans. ARO often associated with impaired vision most likely because nerve compression at optic canal. We examined ocular phenotype mice deficient Methodology/Principal Findings X-ray microtomography showed narrowed foramina skull,...
The production of the cytotoxic agent tripdiolide employing plant tissue cultures TRIPTERYGIUM WILFORDII is demonstrated. Detailed investigations a developed cell line T. in terms versus variations growth conditions, for example media composition, are presented. Also age and size inoculum incubation period with respect to formation evaluated.
Abstract Vacuolar-type ATPase (V-ATPase) is a primary proton pump with versatile functions in various tissues. In nerve cells, V-ATPase required for accumulation of neurotransmitters into secretory vesicles and subsequent release at the synapse. Neurons express specific isoform ( G 2) subunit constituting catalytic sector enzyme complex. Using gene targeting, we generated mouse lacking functional 2 null), which showed no apparent disorders architecture behavior. 2-null brain, 1 isoform,...
ABSTRACT During excystment of an amoeba, Cochliopodium sp., scale formation was examined with light and electron microscopy. This amoeba covered scales. When the encysted, scales remained on external surface cyst wall. Soon after induction Golgi complex began to develop. Many vesicles were extruded from it changed into vacuoles. Scales observed first in vacuole adjacent later inside excysted had been coated by newly formed It is suggested that dependent activity complex.
A retrospective study of 155 patients with submucosal gastric carcinoma compared the clinicopathologic features mucosal and muscularis proprial carcinoma. Fifty-seven percent presented gastrointestinal symptoms, whereas 36.1% had been detected by mass screening. The incidence curative resection, lymph node metastasis, complications were 96.1, 20.6, 14.8%, respectively. Two died sepsis pulmonary infarction 30 days postoperatively. Five recurrent cancer 1-5 years postresection. overall 5-year...
The role of transcription termination process for gene expression regulation is poorly understood. Either a multicopy supply the rof or bicyclomycin, both which inhibit Rho factor, suppressed increased sensitivity to oxidative stress rifampicin‐resistant rpoB mutation in Escherichia coli . Multi‐copy rnk also sensitivity, coincident with recovery reduced concentration nucleoside triphosphates mutant cells, one factors that affects efficiency vitro Thus, an appropriate, nonexcessive frequency...