The abscisic acid (ABA) signalling core in plants include the cytosolic ABA receptors (PYR/PYL/RCARs), clade-A type 2C protein phosphatases (PP2CAs), and subclass III SNF1-related kinases 2 (SnRK2s). aim of this work was to identify these perception system components sweet orange determine influence endogenous on their transcriptional regulation during fruit development ripening, taking advantage comparative analysis between a wild-type fruit-specific ABA-deficient mutant. Transcriptional...

The expansion of aerial organs in plants is coupled with the synthesis and deposition a hydrophobic cuticle, composed cutin waxes, which critically important limiting water loss. While abiotic stress-related hormone abscisic acid (ABA) known to up-regulate wax accumulation response drought, hormonal regulation cuticle biosynthesis during organ ontogeny poorly understood. To address hypothesis that ABA also mediates formation development, we assessed effect deficiency on three...

Citrus fruit ripening is coupled with the synthesis and deposition of epicuticular waxes, which reduces water loss during postharvest storage. Although abscisic acid (ABA) a major regulator citrus ripening, whether ABA mediates wax formation this process remains poorly understood. We investigated implication in cuticle properties metabolism, composition, morphology by comparing Navelate orange [ sinensis (L.) Osbeck] its biosynthesis-impaired mutant Pinalate four stages. deficiency had minor...

Water stress affects many agronomic traits that may be regulated by the phytohormone abscisic acid (ABA). Within these traits, loss of fruit quality becomes important in citrus cultivars develop peel damage response to dehydration. To study dehydration transcriptional responsiveness harvested and putative role ABA this process, performed a comparative large-scale analysis water-stressed fruits wild-type Navelate orange (Citrus sinesis L. Osbeck) its spontaneous ABA-deficient mutant Pinalate,...

Abstract Citrus fruit ripening is a complex process involving biochemical, physiological and molecular events that differ between the flesh peel of fruit. We characterized sweet orange maturation by means comparative transcriptomic analysis Navelate ( sinensis L. Osbeck) its mutant Pinalate, which presents severe blockage at early steps carotenoid biosynthetic pathway consequently reduced ABA levels. Peel involved decrease photosynthetic activity transmembrane transport processes, as well...

The involvement of jasmonic acid (JA), different JA-related metabolites, salicylic (SA), indole-3-acetic (IAA) and abscisic (ABA) in the heat-induced cross-adaptation to chilling has been investigated citrus fruit. effects heating 'Fortune' mandarins at 37 ºC, storing them a temperature (2 ºC) after being exposed, or not, heat-conditioning treatment (3 d on levels these signalling molecules were examined. Jasmonate metabolism activation ºC was followed by that SA, then rise IAA drop ABA....

ABA is involved in plant responses to non-optimal environmental conditions, including nutrient availability. Since copper (Cu) a very important micronutrient, unraveling how affects Cu uptake and distribution relevant ensure adequate nutrition plants subjected stress conditions. Inversely, knowledge about the nutritional status can interfere with biosynthesis signaling mechanisms necessary optimize tolerance horticultural crops. Here reciprocal influence between content was addressed by...

Abstract Copper (Cu) deficiency affects iron (Fe) homeostasis in several plant processes, including the increased Fe requirements due to cuproprotein substitutions for corresponding counterpart. Loss-of-function mutants from Arabidopsis thaliana high affinity copper transporter COPT5 and transporters NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 3/4 (NRAMP3 NRAMP4) were used study interaction between metals internal pools. A physiological characterisation showed that copt5 mutant is...

Relative humidity (RH) during conservation and the chemical composition of epicuticular wax layer are factors that determine fruit quality weight loss. This study investigates influence RH on metabolism citrus storage, how it is affected by abscisic acid (ABA). Low conditions increased alcohols fatty acids abundance, mainly due to accumulation docosanol lignoceric cerotic acids. also decreased terpenoids nonacosane hentriacontane contents, most abundant alkanes. Consequently,...

Epicuticular waxes are important natural compounds that influence cuticle properties and can protect fruit from factors harm its external quality. We demonstrated that, at a dose reduces postharvest citrus quality loss (4 d 2 µL L-1), ethylene redirected epicuticular wax metabolism towards the synthesis of primary alcohols, mostly behenyl alcohol, by favouring acyl-reduction pathway. This treatment also reduced terpenoids redirecting mevalonate pathway farnesol accumulation to detriment most...

The phytohormone abscisic acid (ABA) is a major regulator of fruit response to water stress, and may influence cuticle properties wax layer composition during ripening. This study investigates the effects ABA on epicuticular metabolism regulation in citrus cultivar with low levels, called Pinalate (Citrus sinensis L. Osbeck), how this relationship influenced by stress after detachment. Harvested ABA-treated were exposed storing them at (30-35%) relative humidity. total load rose detachment,...

Histone acetylation affects several aspects of gene regulation, from chromatin remodelling to expression, by modulating the interplay between and key transcriptional regulators. The exact molecular mechanism underlying patterns crosstalk with other epigenetic modifications requires further investigation. In budding yeast, these markers are produced partly histone acetyltransferase enzymes, which act as multi-protein complexes. Sas3-dependent NuA3 complex has received less attention than...

Copper (Cu) is an essential micronutrient for plants because it functions as a redox-active cofactor in vital processes inside the cells. Arable lands are often deficient contents and require application of enriched fertilisers, whose overuse poses high risk human health, environment food safety. Here, we aimed to decipher effects Cu deficiency during fruit growth on other micronutrients nutritional value quality tomato, most consumed worldwide, throughout maturation process. Changes...

Harvested fruit undergo carbon and energy deprivation. However, the events underlying this energy-related stress in detached their involvement cell damage have not yet been elucidated. We showed that supplementing sweet oranges with additional or sources reduced peel damage, while inhibitors of metabolism increased it. investigated effect an exogenous source (glycerol), (ATP), inhibitor 2-deoxy-D-glucose (DeOGlc) + sodium iodoacetate (IAc), on transcriptome harvested flavedo (outer part)....

We investigated whether phospholipases play a role in citrus fruit susceptibility to be infected by Penicillium digitatum, and connection exists between hormone abscisic acid (ABA) the fruit-P. digitatum interaction. Changes both activity of enzymes PLD, PLC PLA2 expression set genes encoding them response infection Navelate (Citrus sinensis (L.) Osbeck) orange its ABA-deficient mutant Pinalate, which is less resistant infection, were compared. The results showed activation PLD before...