A5072509313- Immune Cell Function and Interaction
- Malaria Research and Control
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Neuropeptides and Animal Physiology
- Complement system in diseases
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Hepatitis B Virus Studies
- Gastrointestinal motility and disorders
- Hepatitis C virus research
- Influenza Virus Research Studies
- vaccines and immunoinformatics approaches
- HIV/AIDS Research and Interventions
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 and Mental Health
- Infection Control and Ventilation
- Helicobacter pylori-related gastroenterology studies
- Extracellular vesicles in disease
- Ion Channels and Receptors
- Long-Term Effects of COVID-19
Walter and Eliza Hall Institute of Medical Research
2015-2025
The University of Melbourne
2015-2025
University of California, San Francisco
2008-2016
James Cook University
2015
Australian Institute of Tropical Health and Medicine
2015
San Francisco General Hospital
2012-2015
Malmö University
2003-2009
Lund University
2003
STING is an innate immune cytosolic adaptor for DNA sensors that engage malaria parasite (Plasmodium falciparum) or other pathogen DNA. As P. falciparum infects red blood cells and not leukocytes, how reaches such host in unclear. Here we show parasites inside can cell receptors from a distance by secreting extracellular vesicles (EV) containing parasitic small RNA genomic Upon internalization of DNA-harboring EVs human monocytes, released within the cytosol, leading to STING-dependent...
Naturally acquired humoral immunity to the malarial parasite Plasmodium falciparum can protect against disease, although precise mechanisms remain unclear. Although antibody levels be measured by ELISA, few studies have investigated functional assays in relation clinical outcomes. In this study we applied a recently developed assay of antibody-mediated opsonisation merozoites, plasma samples from longitudinal cohort conducted malaria endemic region Papua New Guinea (PNG). Phagocytic activity...
Severe malaria (SM) is associated with high levels of cytokines such as tumor necrosis factor (TNF), interleukin 1 (IL-1), and 6 (IL-6). The role chemokines less clear, their cellular source.In a case-control study children SM (n = 200), uncomplicated (UM) 153) healthy community controls (HC) 162) in Papua, New Guinea, we measured cytokine/chemokine production by peripheral blood mononuclear cells (PBMCs) stimulated live Plasmodium falciparum parasitized red (pRBC). Cellular sources were...
Pathogens can release extracellular vesicles (EVs) for cell–cell communication and host modulation. EVs from Plasmodium falciparum, the deadliest malaria parasite species, transfer drug resistance genes between parasites. late-stage parasite-infected RBC (iRBC-EVs) are immunostimulatory affect endothelial cell permeability, but little is known about early stage iRBC. We detected virulence factor PfEMP1, which responsible iRBC adherence a major contributor to disease severity, in EVs, only up...
It is unclear whether naturally acquired immunity to Plasmodium falciparum results from the acquisition of antibodies multiple, diverse antigens or fewer, highly conserved antigens. Moreover, specific antibody functions required for malaria are unknown, and hence informative immunological assays urgently needed address these knowledge gaps guide vaccine development. In this study, we investigated merozoite-opsonizing associated with protection in a strain-specific strain-transcending manner...
γδ T cells are important for both protective immunity and immunopathogenesis during malaria infection. However, the immunological processes determining beneficial or detrimental effects on disease outcome remain elusive. The aim of this study was to examine expression regulatory effect inhibitory receptor T-cell immunoglobulin domain mucin 3 (TIM3) cells. While TIM3 function conventional αβ have been clearly defined, equivalent characterization associations with outcomes is limited. This...
The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In present study, NTHi adherence to epithelial cells various origins was further analyzed. number intraepithelial PE-deficient decreased compared with PE-expressing NTHi. Interestingly, or Escherichia coli transformants, addition soluble recombinant PE22-160 without a lipid moiety, induced proinflammatory cell response. adhesive PE domain defined within...
Toll-Like Receptors (TLRs) are important mediators of the innate immune response to pathogens, including malaria.Of ten human and twelve mouse TLRs, TLR2, TLR4, TLR7 TLR9 known detect malarial antigens induce anti-malarial responses.Multiple cell populations express much has been done elucidate TLR-mediated malaria infections, in particular involvement TLRs severe pathogenesis.Here we review role play parasite detection, response, malaria, with a focus on recent findings.Furthermore, use TLR...
In this article, we discuss a recent article by Jia et al., where high OLAH expression was detected in severe and fatal respiratory disease which associated with number of processes responses. These include abundance oleic acid, excessive cytokine release, viral titres lipid droplets increased presence lung-associated innate cells.
Solid tumor malignancy (STM) patients experience increased risk of breakthrough SARS-CoV-2 infection owing to reduced COVID-19 vaccine immunogenicity. However, the underlying immunological causes impaired neutralization remain poorly characterized. Furthermore, non-neutralizing antibody functions can contribute disease severity but understudied within high-risk populations. We dissected polyfunctional responses in STM and age-matched controls who received adenoviral vector- or mRNA- based...
ABSTRACT Immunity to Plasmodium falciparum malaria is slow develop, and it often asserted that suppresses host immunity, although this poorly understood the molecular basis for such activity remains unknown. P. erythrocyte membrane protein 1 (PfEMP1) a virulence factor plays key role in parasite-host interactions. We investigated immunosuppressive effect of PfEMP1 on monocytes/macrophages, which are central antiparasitic innate response. RAW macrophages human primary monocytes were...
Background Antibodies targeting merozoites are important in protection from malaria. Therefore, merozoite surface proteins attractive vaccine candidates. There is a need for robust functional assays to investigate mechanisms of acquired immunity and efficacy. To date, the study phagocytosis has been confounded by complexity variability vitro assays. Methodology/Principal findings We have developed new flow cytometry-based assay. An optimized preparation technique produced high yields...
Eosinophils are a characteristic component of the inflammatory response seen in several diseases, including allergic asthma and chronic obstructive pulmonary disease. After activation, eosinophil-derived products may exert proinflammatory effects cause considerable tissue damage. In present study, we investigated innate interactions between respiratory tract pathogen nontypeable Haemophilus influenzae (NTHi) human eosinophils. Bacterial binding to eosinophils was dependent on...