Objective— Chronic systemic inflammation accompanies obesity and predicts development of cardiovascular disease. Dietary cholesterol has been shown to increase atherosclerosis in LDL receptor–deficient (LDLR −/− ) mice. This study was undertaken determine whether dietary have additive effects on atherosclerosis. Methods Results— LDLR mice were fed chow, high-fat, high-carbohydrate (diabetogenic) diets without (DD) or with added (DDC) for 24 weeks. Effects adipose tissue, inflammatory...

Obesity is characterized by adipocyte hypertrophy and macrophage accumulation in adipose tissue. Monocyte chemoattractant protein-1 (MCP-1) plays a role recruitment into However, other adipocyte-derived factors, e.g., hyaluronan serum amyloid A (SAA), can facilitate monocyte adhesion chemotaxis, respectively. The objective was to test the potential involvement of these factors recruitment. Differentiated 3T3-L1 adipocytes made hypertrophic growth high glucose conditions were used study SAA...

During inflammation, the serum amyloid A (SAA) content of HDL increases, whereas apolipoprotein A-I (apoA-I) and paraoxonase-1 (PON-1) decrease. It remains unclear whether SAA physically displaces apoA-I or if these changes derive from coordinated but inverse transcriptional regulation genes. Because cytokines stimulate hepatic expression inflammatory markers, we investigated their role in regulating SAA, apoA-I, PON-1 expression.A cytokine mixture (tumor necrosis factor [TNF]-alpha,...

To clarify the prevalence of dietary supplement use among college students, we conducted Internet-based nationwide questionnaire surveys with 157,595 Japanese students aged between 18 to 24 years old who were registrants Macromill Inc. (Tokyo, Japan). Among 9066 respondents (response rate 5.8%), 16.8% currently using supplements. The did not differ significantly males (17.1%) and females (16.7%). However, it increased according their grade (13.1% 20.5%), was higher in medical pharmaceutical...

Recently, people have used dietary supplements not only for nutritional supplementation, but also treatment of their diseases. However, use to treat diseases, especially with medications, may cause health problems in patients. In this study, we investigated patients Japan. This survey was conducted from January December 2012, and completed by 2732 people, including 599 admitted patients, 1154 ambulatory 979 healthy subjects who attended a seminar about supplements. At the time questionnaire,...

Adipose tissue secretes proteins like serum amyloid A (SAA), which plays important roles in local and systemic inflammation. Circulating SAA levels increase obese humans, but the of adipose-derived hyperlipidemia this process are unclear. We took advantage difference inducible isoforms secreted by adipose (SAA3) liver (SAA1 2) mice to evaluate whether contributes circulating pool obesity hyperlipidemia. Genetically (ob/ob) mice, not hyperlipidemic deficient apolipoprotein E (Apoe(-/-)), had...

In Japan, dietary habits have greatly changed since the coronavirus disease (COVID-19) outbreak; we examined factors related to changes. An online cross-sectional questionnaire survey was conducted in November 2020 among 6000 Japanese adults (aged 20–64 years) registered with a research company and gathered data on demographics, socioeconomic factors, medical history, COVID-19 status of respondent’s family neighbors, fear COVID-19, changes lifestyle outbreak. To question “Have you made...

Background It is not clear how hyperbaric oxygen therapy (HBO) affects ischemia-induced pathophysiological responses such as angiogenesis and skeletal muscle regeneration. In the present study effects of HBO on functional morphological recovery ischemic hind limbs, blood perfusion local production angiogenic growth factors were studied in a mouse model. Methods Results Mice placed pure under 3 atm for 1 h/day 14 days after removal segment left femoral artery. HBO-treated mice showed better...

Objective— Levels of serum amyloid A (SAA), an acute-phase protein carried on high-density lipoprotein (HDL), increase in inflammatory states and are associated with increased risk cardiovascular disease. HDL colocalizes vascular proteoglycans atherosclerotic lesions. However, its major apolipoprotein, apolipoprotein A-I, has no proteoglycan-binding domains. Therefore, we investigated whether SAA, which domains, plays a role retention by proteoglycans. Methods Results— from control mice...

We previously reported that some patients used dietary supplements with their medication without consulting physicians. Dietary and medicines may interact each other when concomitantly, resulting in health problems. An Internet survey was conducted on 2109 people who concomitantly took order to address supplement usage regularly take Japan. A total of 1508 (two admitted 1506 ambulatory patients) 601 non-patients, were not physicians, participated this study. Purpose for use different among...

Non-alcoholic steatohepatitis (NASH) is a globally recognized liver disease. A methionine- and choline-deficient diet used to induce NASH in mice; however, this also causes severe body weight loss. To resolve issue, we examined the effects of methionine content high-fat (HFCD) on development mice.C57BL/6J mice (male, 10 weeks age) were fed an L-amino acid rodent (control) diet, (HF) or HFCD containing various amounts (0.1-0.6% (w/w)) for 12 weeks. Plasma lipid levels, hepatic inflammatory...

Short-chain fatty acids, including acetate, propionate, and butyrate are metabolites of dietary fibre produced by microbiota in the large intestine, have been proposed to contribute effects on bone homeostasis. However, it is unclear whether they used osteoblasts directly affect formation. We investigated short-chain acids absorbed osteoblast cells influence early osteoblastic differentiation using MC3T3-E1 cells. Acetate propionate upregulated alkaline phosphatase activity, which an marker,...

To clarify the role of very low density lipoprotein (VLDL) and apolipoprotein E (apoE) in adipogenesis, we studied newly developed hyperlipidemic obese (ob/ob;apoE-/-) mice. Because hydrolysis VLDL is believed to be major source adipogenic free fatty acids, a higher plasma level these mice should exaggerate obesity.When fed high-fat, high-cholesterol diet, ob/ob;apoE-/- did not show increased body weight or an amount adipose tissue spite levels, whereas ob/ob showed tissue, suggesting that...

We have previously reported on the prevalence of dietary supplements among college students; it was deduced that their intake increased according to grade (i.e., 13.1% in first 20.5% sixth grade). also some students had experienced adverse events Japan due these supplements. However, awareness remains limited, even pharmaceutical students. Being appropriately educated about them is important for students, both themselves as well future careers pharmacists.We conducted a lecture-based...

Lifestyle changes during the coronavirus disease (COVID-19) lockdown have been previously examined, but there is limited understanding about after such restrictions were lifted. This study examines in lifestyle habits and body weight among Japanese population with regard to length of at-home hours both (April May) (September) nationwide stay-at-home request compared those before COVID-19 pandemic (January 2020). An online survey was conducted September 2020 involving 10,000 monitors,...

Abstract Trust in messengers is essential for a public agency to ensure effective benefit-risk communication. However, there insufficient research on the difference trust between risk-only or benefit-and-risk messages that deal with negative topics. To clarify these differences, this study used three radiation topics determine best communication design. We conducted randomized comparative November 2020 1100 Japanese individuals (550 men and 550 women), who were allocated either risk message...

Senescence marker protein‐30 (SMP30) was originally identified as a novel protein in the rat liver, expression of which decreases androgen‐independently with aging. We have now characterized unique property SMP30, hydrolysis diisopropyl phosphorofluoridate (DFP), is similar to chemical warfare nerve agents sarine, soman and tabun. Hydrolysis DFP stimulated equally well by 1 mM MgCl 2 , MnCl or CoCl lesser extent CdCl but not at all CaCl . No 45 Ca 2+ ‐binding activity detected for purified...

The prevalence of dietary supplements has increased in Japan, and, as a consequence, the adverse events associated with supplement use have become more prominent. Severe must be reported to Japanese government via public health centers. However, number cases is limited. To clarify this discrepancy, we conducted an internet questionnaire, and surveyed how consumers, physicians pharmacists acted when they or their patients developed due use. This study was completed by 2732 515 physicians,...

Aim: Resveratrol is a popular ingredient in dietary supplements. Some patients concomitantly use supplements and medicines Japan. In the present study, we determined whether trans-resveratrol melinjo (Gnetum gnemon L.) seed extract (MSE), which contains resveratrol dimers, interacted with drugs using mouse model.