Human exposure to genotoxic compounds present in ambient air has been studied using selected biomarkers nonsmoking Danish bus drivers and postal workers. A large interindividual variation biomarker levels was observed. Significantly higher of bulky carcinogen-DNA adducts (75.42 adducts/10(8) nucleotides) 2-amino-apidic semialdehyde (AAS) plasma proteins (56.7 pmol/mg protein) were observed working the central part Copenhagen, Denmark. In contrast, significantly AAS hemoglobin (55.8 protein),...

Interaction between tumour survival tactics and anti-tumour immune response is a major determinant for cancer growth. Regulatory T cells (T-regs) contribute to escape, but their role in basal cell carcinoma (BCC) not understood. The fraction of T-regs among was analysed by immunohistochemistry followed automated image analysis facial BCC, peritumoural skin normal, buttock skin. Quantitative real-time PCR (qRT-PCR) performed Foxp3 cytokines involved T-reg attraction T-cell activation....

The tumour microenvironment in classical Hodgkin's lymphoma (cHL) is characterised by a minor population of neoplastic Hodgkin and Reed-Sternberg cells within heterogeneous background non-neoplastic bystanders cells, including mast cells. number infiltrating cHL has been reported to correlate with poor prognosis. We used immunohistochemistry assess the degree tumour-infiltrating tissue microarrays correlated this clinico-pathological features prognosis cohort homogeneously treated patients...

Distinction between benign and malignant melanocytic lesions may be difficult by today's methods, even for highly skilled dermatopathologists, emphasizing the need improved diagnostic tools. We have studied discriminative abilities of immunohistochemical (IHC) double stains using IHC markers Ki67 combined with MART1, HMB45 MITF. Paraffin-embedded tissue sections from 50 melanomas 78 nevi were stained a simple simultaneous staining technique. Both semiquantitative estimates immunopositivity...

The increased risk of cardiovascular diseases in type 2 diabetes mellitus has been extensively documented, but the origins association remain largely unknown. We sought to determine changes protein expressions arterial tissue from patients with and moreover hypothesized that metformin intake influences composition.We analyzed nonatherosclerotic repair arteries gathered at coronary bypass operations 30 age- sex-matched nondiabetic individuals. Quantitative proteome analysis was performed by...

Post-transplant lymphoproliferative disorders (PTLDs) are potentially fatal, often Epstein-Barr virus (EBV)-driven neoplasias developing in immunocompromised hosts. Initial treatment usually consists of a reduction immunosuppressive therapy and/or rituximab with or without chemotherapy. However, patients who relapse do poorly, and new options warranted. With the introduction immunoconjugate brentuximab vedotin, CD30 antigen has become an effectively targetable molecule. Therefore, we...

Aims: Seafarers aboard oil and chemical tankers may be exposed to many chemicals, including substances like benzene that are known carcinogenic. Other seafarers engine exhaust, different products, chemicals used some years ago asbestos was also extensively in ships. The aim of this study cancer morbidity among Danish relation type ship job title. Methods: A cohort all during 1986–1999 (33 340 men; 11 291 women) registered by the Maritime Authority with an employment history linked nationwide...

The proliferation marker Ki67 is an important diagnostic and prognostic aid in surgical pathology. However, manual quantification a counting frame to accurately establish the rate (Ki67 index) cumbersome time-consuming. Instead, digital image analysis of Ki67/MART1 double stains may provide fast novel index computations for entire tumor sections.To design compare protocols that compute indices stains, automated with previously published indices, total number proliferating cells (mimicking...

Systemic inflammation measured by the acute-phase protein CRP associates with poor outcome across cancer types. In contrast, local tumor-associated inflammation, primarily evaluated T-lymphocytes, correlates favorable prognosis. Yet, little is known whether these two responses are related or opposing processes and why elevated in relation to detrimental for clinical outcome. As proof of concept, we developed a platform combining multiplexed IHC digital imaging, enabling virtual readout both...

Staging of melanoma includes quantification a proliferation index, i.e., presumed melanocytic mitoses H&E stains are counted manually in hot spots. Yet, its reproducibility and prognostic impact increases by immunohistochemical dual staining for phosphohistone H3 (PHH3) MART1, which also may enable fully automated image analysis. To ensure manageable workloads repeatable measurements modern pathology, the study aimed to present an with hot-spot selection PHH3/MART1-stained melanomas....

Immune therapy improves cancer outcomes, yet many patients do not respond. This pre-clinical study investigated whether vascular disrupting agents (VDAs) could convert an immune unresponsive tumor into a responder. CDF1 mice, with 200 mm3 C3H mammary carcinomas in the right rear foot, were intraperitoneally injected combretastatin A-4 phosphate (CA4P), its A-1 analogue OXi4503, and/or checkpoint inhibitors (anti-PD-1, PD-L1, or CTLA-4 antibodies), administered twice weekly for two weeks....

Pathologists often use immunohistochemical staining of the proliferation marker Ki67 in their diagnostic assessment melanocytic lesions. However, interpretation can be challenging. We propose a new workflow to improve utility Ki67-index. In this workflow, is combined with tumour-cell SOX10 Ki67/SOX10 double nuclear stain. The Ki67-index then quantified automatically using digital image analysis (DIA). aim study was optimise and test three different multiplexing methods for staining.

In this proof-of-concept study, we propose a new method for automated digital quantification of PRAME (PReferentially expressed Antigen MElanoma) as diagnostic aid to distinguish between benign and malignant melanocytic lesions. The proposed utilizes immunohistochemical virtual double nuclear staining SOX10 precisely identify the cells interest, which is combined with image analyse quantify PRAME-index.

Background Systemic inflammation, diagnostically ascribed by measuring serum levels of the acute phase reactant C-reactive protein (CRP), has consistently been correlated with poor outcomes across cancer types. CRP exists in two structurally and functionally distinct isoforms, circulating pentameric (pCRP) highly pro-inflammatory monomeric isoform (mCRP). The aim this pilot study was to map pattern mCRP distribution a previously immunologically well-defined colon (CC) cohort explore possible...

Inability to distinguish melanomas from benign nevi is the most frequent reason for malpractice lawsuits in surgical pathology. Reliable diagnostic tools support hematoxylin and eosin (H&E) stains induce vigilance are thus highly needed. Because high performance recently was showed using automated image analysis, immunohistochemical proliferation marker Ki67 seems a potential candidate. This study aimed investigate if this previously presented algorithm could have prevented 10 false-negative...

Deep learning for the analysis of H&E stains requires a large annotated training set. This may form labor-intensive task involving highly skilled pathologists. We aimed to optimize and evaluate computer-assisted annotation based on digital dual same tissue section. primary metastatic melanoma (N = 77) were digitized, re-stained with SOX10, re-scanned. Because images aligned, annotations SOX10 image directly transferred Based 1,221,367 nuclei, convolutional neural network calculating tumor...

Telomerase is reactivated in most cancers and possibly an early driver event melanoma. Our aim was to test a novel situ hybridization technique, RNAscope, for the detection of human telomerase reverse transcriptase (hTERT) mRNA archival formalin-fixed, paraffin-embedded (FFPE) tissue compare expression melanomas benign naevi. Furthermore, we wanted see if hTERT could be diagnostic or prognostic marker In performed on FFPE 17 13 We found significant difference between naevi ( P <0.001)...

Ki-67 immunohistochemical expression is a prognostic and predictive marker in many breast cancer studies. Instead of the conventional time-consuming score single stains associated with low reproducibility, Ki-67/KL1 double may facilitate fast, repeatable quantification by digital image analysis. This study aims to detect difference accuracy precision between manual indices stains, develop an automated explore relation tumor characteristics when quantified different regions: hot spots, global...