A5029157020- Biochemical and Molecular Research
- Fungal and yeast genetics research
- Enzyme Structure and Function
- Influenza Virus Research Studies
- Genetic Neurodegenerative Diseases
- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Cancer Research and Treatments
- HIV/AIDS drug development and treatment
- Bone health and osteoporosis research
- Bone Metabolism and Diseases
- Bone health and treatments
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Virus-based gene therapy research
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- DNA Repair Mechanisms
Mahidol University
2011-2024
Abstract The highly pathogenic avian influenza A (H5N1) virus is a virulent that causes an acute febrile respiratory disease with high mortality in humans. To gain better insight of H5N1 viral distributions infected human tissues, the levels RNA were determined autopsy tissues from two patients who by using real‐time reverse transcription‐polymerase chain reaction. In one patient died on day 6 illness, load lung was extremely high, whereas other organs more than log lower. 17 similar and...
Nevirapine (NVP) is a non-nucleoside reverse transcriptase inhibitor frequently used in combination with other antiretroviral agents for highly active therapy (HAART) of patients infected the human immunodeficiency virus type 1 (HIV-1). However NVP can cause serious, life-threatening complications. Hepatotoxicity one most severe adverse effects, particularly HIV chronic hepatitis C co-infection as these develop liver toxicity after relatively short course treatment. However, mechanism...
Abstract Recent work has found that many metabolic enzymes have the ability to polymerize in response changes or environmental stress. This is well conserved for few enzyme paralogs been studied yeast. Here we describe first set of paralogs, Asn1p and Asn2p, differential assembly behavior. Asn2p both co-assemble into filaments nutrient limitation. However, form strictly dependent on presence Asn1p. Using mutations block activity but effects polymerization, polymers are unlikely acquired a...
Previously, we have developed an extramitochondrial assembly system, where mitochondrial targeting signal (MTS) can be removed from a given enzyme, which could used to characterize the regulatory factors involved in enzyme assembly/disassembly vivo Here, demonstrate that addition of exogenous acetaldehyde quickly induce supramolecular MTS-deleted aldehyde dehydrogenase Ald4p yeast cytoplasm. Also, by using PCR-based modification genome, cytoplasmically targeted cannot polymerize into long...
Recently, human asparagine synthetase has been found to be associated with the mitotic spindle. However, this event cannot seen in yeast because takes a different cell division process via closed mitosis (there is no nuclear envelope breakdown allow association between any cytosolic enzyme and spindle). To find out if can also (but hiddenly) have feature, coding sequences of green fluorescent protein (GFP) localization signal (NLS) were introduced downstream ASN1 ASN2, encoding synthetases...
Previously, we identified yeast strains, namely SWORD, showing more robust Ald4p-GFP filament formation than the typical ALD4::GFP strains. Here, report that in SWORD strains favorably polymerize into gigantic structures cytoplasm, despite enzyme being established as a mitochondrial resident. In addition, have found nocodazole, microtubule destabilizer, has no effect on Ald4p high-order assembly, suggesting direct association between dynamics and structure formation. assembly cannot be...
As part of our studies yeast aldehyde dehydrogenase (Ald4p) assembly, we identified a population transformants (SWORD strain) that show more robust filament formation GFP-tagged Ald4p (Ald4p-GFP) than wild type ALD4::GFP strain. Sequencing the ALD4 gene in SWORD strain showed increased assembly was not due to changes coding sequence, suggesting second mutation site altering assembly. Using short-read whole-genome sequencing, spontaneous mutations FLO9. Introduction allele FLO9 into wild-type...
Iron overload negatively affects bone mass and strength. However, the impact of iron excess on osteocytes-important cells for mechanotransduction remodeling-is poorly understood. Herein, we examined effects exposure osteocytes during their maturation process. We discovered that caused apoptosis in early late stages differentiation. Notably, expression key proteins entry was downregulated differentiation, suggesting mature were less susceptible to toxicity due limited uptake. Furthermore,...
Influenza A virus (IAV) infection in pregnant women is a major public health concern. However, the effect of IAV on human embryogenesis still unclear. Here we show that induced pluripotent stem cells (hiPSCs) and hiPSC-derived ectodermal, mesodermal endodermal are susceptible to infection. These cell types stained positive for α2,6-linked sialic acid, receptor expressed surface. While hiPSCs produced high viral titers up 7 days with increasing infected number suggesting progenies from...