A5011471105- Science, Research, and Medicine
- Immunotherapy and Immune Responses
- Salmonella and Campylobacter epidemiology
- Parasitic Infections and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- Cellular transport and secretion
- Virus-based gene therapy research
- Transgenic Plants and Applications
- Viral Infections and Vectors
- DNA Repair Mechanisms
- Viral Infections and Outbreaks Research
- Cell Adhesion Molecules Research
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Bacteriophages and microbial interactions
- Microbial Inactivation Methods
- Carcinogens and Genotoxicity Assessment
- Escherichia coli research studies
- Viral Infectious Diseases and Gene Expression in Insects
- Fire effects on ecosystems
- Skin and Cellular Biology Research
- Antibiotic Resistance in Bacteria
- Cystic Fibrosis Research Advances
Animal and Plant Health Agency
2013-2017
Ealing Hospital
2006
European Molecular Biology Laboratory
2000
University of Cambridge
1998-1999
The Gurdon Institute
1998-1999
Myerscough College
1995
Christ University
1995
Scripps Clinic
1988-1991
Scripps Health
1988-1991
Johns Hopkins Medicine
1988
The cdc25+ gene of fission yeast encodes a phosphotyrosine phosphatase that dephosphorylates tyrosine-15 p34cdc2 and thereby activates p34cdc2/cyclin to bring about entry into M phase. We have recently cloned human homolog, CDC25, which rescues the M-phase initiation defect cdc25 temperature-sensitive mutants. Antibodies raised against CDC25 product specifically recognize proteins approximately 55 52 kDa. Microinjection affinity-purified anti-CDC25 antibodies HeLa cells inhibits mitosis....
A monoclonal antibody that reacts with proteins in the nuclear pore complex of rat liver (Snow, C. M., A. Senior, and L. Gerace. 1987. J. Cell Biol. 104:1143-1156) has been shown to cross react similar components Xenopus oocytes, as determined by immunofluorescence microscopy immunoblotting. We have microinjected into oocytes study possible role these polypeptides nucleocytoplasmic transport. The inhibits import a large protein, nucleoplasmin, time- concentration-dependent manner. It also...
Newly synthesized G protein of vesicular stomatitis virus is not transported to the surface cultured mammalian cells during mitosis (Warren et al., 1983, J. Cell Biol. 97:1623-1628). To determine where intracellular transport inhibited, we have examined post-translational modifications protein, which are indicators specific compartments on pathway. in mitotic had only endo H-sensitive oligosaccharides containing seven or eight mannose residues, but no terminal glucose, and was fatty...
Giardia duodenalis is a common intestinal parasite in humans and wide range of livestock species. It genetically heterogeneous that has been characterized seven distinct genetic assemblages or cryptic species, molecular markers can be used to differentiate both animal-specific potentially zoonotic genotypes. Little known about G. the occurring domestic species UK. Here, we present data on occurrence diversity detected faeces large contents cattle, sheep, pigs, goats camelids from farms...
Indirect immunofluorescence, immunoelectron microscopy, and digestion by protease were used to study intracellular transport of the G protein vesicular stomatitis virus in mitotic interphase cells. Quantitation showed that appearance on surface cells was inhibited at least 10-fold when compared with cells, even though similar amounts viral being synthesized. This dramatic inhibition, taken together simultaneous inhibition endocytosis (Berlin, R. D., J. M. Oliver, 1980, Cell Biol. 85:...
SUMMARY Five cases of STEC O157 phage type (PT) 21/28 reported consumption raw cows' drinking milk (RDM) produced at a dairy farm in the South West England. PT21/28 was isolated from faecal specimens milking cows on implicated farm. Whole genome sequencing (WGS) showed that human and cattle isolates were same strain. Further analysis WGS data confirmed sequences an additional four (who did not report RDM when first questioned) fell within five single nucleotide polymorphism cluster as...
We report the detection of a strain Seoul hantavirus (SEOV) in pet rats England and Wales. The discovery followed an investigation case haemorrhagic fever with renal syndrome Hantavirus RNA was detected via real-time reverse transcription-polymerase chain reaction (RT-PCR) classic RT-PCR belonging to patient. Sequencing phylogenetic analysis confirmed virus be SEOV that is similar, but not identical, previously reported United Kingdom from wild rats.
SUMMARY As part of further investigations into three linked haemorrhagic fever with renal syndrome (HFRS) cases in Wales and England, 21 rats from a breeding colony Cherwell, household Cheltenham were screened for hantavirus. Hantavirus RNA was detected either the lungs and/or kidney 17/21 (81%) Cherwell tested, higher than previously by blood testing alone (7/21, 33%), kidneys all rats. The partial L gene sequences obtained 10 identical to each other reported UK strain. Seoul hantavirus...
Fifteen confirmed cases and 15 possible of Shiga toxin-producing Escherichia coli (STEC) O157 phage type 21/28 were linked to direct contact with lambs at a 'Lambing Live' event in the North West England between 29 March 21 April 2014. Twenty-one (70%) female, 23 (77%) children aged <16 years, whom 14 (46%) 0-5 years age group. Five developed haemolytic uraemic syndrome. Multilocus variable number tandem repeat analysis (MLVA) profiles on human indistinguishable, 6/10 animal isolates had...
Veterinary RecordVolume 163, Issue 20 p. 599-600 Short Communication Human Leptospira interrogans serogroup icterohaemorrhagiae infection (Weil's disease) acquired from pet rats C. M. Gaudie BVMS, MRCVS, MRCVS Laboratories Agency (VLA) — Thirsk, Station Road, North Yorkshire, YO7 1PZSearch for more papers by this authorC. A. Featherstone MA, VetMB, authorW. S. Phillips MB, ChB, MPH, FFPHM, W. FFPHM South Yorkshire Health Production Unit, Protection Agency, Sheffield, S9 1BYSearch authorR....
Following the introduction of a national abattoir-based monitoring programme for Salmonella in pigs, advisory visits were made to pig farms England and Wales with high seroprevalence assessed by muscle tissue fluid (meat juice) ELISA. Samples (n = 15 790), including pooled pen floor faeces 12 136), collected culture from 296 farms, between October 2003 February 2008. was isolated 4489 (28%) all samples collected, 3301 (27%) faecal samples, 270 (91%) visited. Typhimurium S. Derby most...
SUMMARY A cross-sectional study into risk factors for Salmonella was undertaken using data gathered from 252 fattening turkey flocks in the UK. The derived EU baseline survey conducted during 2006 and 2007, addition to a voluntary questionnaire. Multivariate logistic regression models identified significant spp. Typhimurium. decreased of infection associated with history intestinal illness sampled flock (OR 0·17), use wood shavings as litter 0·21), disinfectant cleaning process 0·25),...