A5029559432- Rabies epidemiology and control
- Hepatitis B Virus Studies
- Lymphoma Diagnosis and Treatment
- Viral Infections and Vectors
- Microbial infections and disease research
- Viral-associated cancers and disorders
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Hepatitis C virus research
- Streptococcal Infections and Treatments
- Viral Infections and Outbreaks Research
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Bat Biology and Ecology Studies
- Genetic factors in colorectal cancer
- Chronic Lymphocytic Leukemia Research
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Cancer-related molecular mechanisms research
- CAR-T cell therapy research
- Liver Disease Diagnosis and Treatment
University of Oxford
2023-2025
Medawar Building for Pathogen Research
2023
RIKEN Center for Integrative Medical Sciences
2022
Animal and Plant Health Agency
2016-2020
<ns4:p>Genomic surveillance is an important aspect of contemporary disease management but has yet to be used routinely monitor endemic transmission and control in low- middle-income countries. Rabies almost invariably fatal viral that causes a large public health economic burden Asia Africa, despite being entirely vaccine preventable. With policy efforts now directed towards achieving global goal zero dog-mediated human rabies deaths by 2030, establishing effective tools critical. Genomic...
Epstein-Barr virus (EBV)-positive Burkitt lymphoma (BL) affects children in sub-Saharan Africa, but diagnosis via tissue biopsy is challenging. We explored a liquid approach using targeted next-generation sequencing to detect the The panel included targets for characteristic detected three of four This analysis demonstrates potential improve BL settings with limited pathology resources. Validation our larger data set needed.
Abstract Hepatitis B virus (HBV) whole genome sequencing (WGS) is currently limited as the DNA viral loads (VL) of many clinical samples are below threshold required to generate full genomes using current methods. We developed two pan-genotypic enrichment methods, probe-based capture and tiled amplicon PCR (HEP-TILE) for HBV WGS. demonstrate mock that both methods (genotypes A-J). Using samples, we HEP-TILE amplification successfully amplifies at lowest VL tested (30 IU/ml), products can be...
Genomic surveillance is an important aspect of contemporary disease management but has yet to be used routinely monitor endemic transmission and control in low- middle-income countries. Rabies almost invariably fatal viral that causes a large public health economic burden Asia Africa, despite being entirely vaccine preventable. With policy efforts now directed towards achieving global goal zero dog-mediated human rabies deaths by 2030, establishing effective tools critical. data can provide...
Summary Immune responses to primary COVID‐19 vaccination were investigated in 58 patients with follicular lymphoma (FL) as part of the PETReA trial frontline therapy (EudraCT 2016–004010‐10). vaccines (BNT162b2 or ChAdOx1) administered before, during after cytoreductive treatment comprising rituximab (depletes B cells) and either bendamustine CD4 + T cyclophosphamide‐based chemotherapy. Blood samples obtained vaccine doses 1 2 (V1, V2) analysed for antibodies cells reactive SARS‐CoV‐2 spike...
SUMMARY As part of further investigations into three linked haemorrhagic fever with renal syndrome (HFRS) cases in Wales and England, 21 rats from a breeding colony Cherwell, household Cheltenham were screened for hantavirus. Hantavirus RNA was detected either the lungs and/or kidney 17/21 (81%) Cherwell tested, higher than previously by blood testing alone (7/21, 33%), kidneys all rats. The partial L gene sequences obtained 10 identical to each other reported UK strain. Seoul hantavirus...
In the United Kingdom, there has been an increase in number of hepatitis E virus (HEV) infections people annually since 2010. Most these are thought to be indigenously acquired Orthohepevirus A genotype 3 (HEV G3), which linked pork production and consumption. However, dominant subgroup circulating British pigs differs from that is found people; therefore, alternative, potentially zoonotic, source suspected as a possible cause infections. Rodents, brown rats (Rattus norvegicus) particular,...
The lyssaviruses are an important group of viruses that cause a fatal encephalitis termed rabies. prototypic lyssavirus, rabies virus, is predicted to more than 60 000 human fatalities annually. burden disease for the other undefined. original reports recently described highly divergent Lleida bat lyssavirus were based on detection virus sequence alone. successful isolation live from carcass and in vitro characterization this novel here. In addition, ability vaccine confer protective...
Rabies is a fatal neurologic disease caused by lyssavirus infection. People are infected through contact with animals. The relative increase of human rabies acquired from bats calls for better understanding infections in their natural hosts. So far, there no experimental model that mimics infection the reservoir bat species. Lagos virus endemic straw-colored fruit (Eidolon helvum) Africa. Here we compared susceptibility these to three strains (from Senegal, Nigeria, and Ghana) intracranial...
Molecular assays are rapid, sensitive and specific, have become central to diagnosing rabies. PCR based been utilized for decades confirm rabies diagnosis but only recently accepted by the OIE (World Organisation Animal Health) as a primary method detect infection. Real-time RT-PCR provide real-time data, closed-tube systems, minimizing risk of contamination during setup. DNA intercalating fluorochrome do not require expensive probes, cost per sample, when primers designed in conserved...
ABSTRACT Hepatitis B Virus (HBV) genome sequencing can be used to provide more complete genetic information at the population and individual level shed light on limitations of current interventions, inform new strategies for elimination. HBV is challenging due partially dsDNA genome, high diversity, low viral loads presence large amounts host material in clinical samples. Here we describe design use a pan-genotypic panel 74 specific capture-probes nuclease treatment improving efficiency. We...
Diagnosing and treating chronic hepatitis B virus (HBV) infection are key interventions to support progress towards elimination of viral by 2030. Although nucleos/tide analogue (NA) therapy is typically highly effective, challenges remain for load (VL) suppression, including medication access, incomplete adherence drug resistance. We present a case long-term HBV HIV coinfected adult prescribed with sequential NA regimens, episodes breakthrough viraemia. Multiple factors contribute...
Hepatitis B virus (HBV) whole genome sequencing (WGS) is currently limited as the DNA viral loads (VL) of many clinical samples are below threshold required to generate full genomes using current methods. We developed two pan-genotypic enrichment methods, probe-based capture and tiled amplicon PCR (HEP-TILE) for HBV WGS. demonstrate mock that both methods (genotypes A-J). Using samples, we HEP-TILE amplification successfully amplifies at lowest VL tested (30 IU/ml), products can be sequenced...
Introduction Hantaviruses are maintained by mammalian hosts, such as rodents, and shed in their excretions. Clinical disease can occur humans from spillover infection. Brown rats ( Rattus norvegicus) the globally distributed reservoir host of Seoul virus (SEOV). Human cases SEOV‐associated haemorrhagic fever with renal syndrome (SEOV‐HFRS)have been reported Great Britain (GB) since 1977. Methods (n=68) were trapped a variety peridomestic locations, focus on pig farms. Kidney lung tissues...
Rabies is a fatal neurologic disease caused by lyssavirus infection. Bats are important natural reservoir hosts of various lyssaviruses that can be transmitted to people. The epidemiology and pathogenesis rabies in bats poorly understood, making it difficult prevent zoonotic transmission. To further our understanding bat host, an experimental model using straw-colored fruit (Eidolon helvum) Lagos virus, endemic this species, was developed. determine the lowest viral dose resulting 100%...