Abstract One of the most promising strategies to treat cancer is use therapeutic antibodies that disrupt cell–cell adhesion mediated by dysregulated cadherins. The principal site where occurs encompasses Trp2 found at N‐terminal region protein. Herein, we employed naturally exposed highly conserved peptide Asp1‐Trp2‐Val3‐Ile4‐Pro5‐Pro6‐Ile7, as epitope prepare molecularly imprinted polymer nanoparticles (MIP‐NPs) recognize Since MIP‐NPs target responsible for adhesion, they were more potent...

Phenotypic variability in isogenic bacterial populations is a remarkable feature that helps them cope with external stresses, yet it incompletely understood. This can stem from gene expression noise and/or the unequal partitioning of low-copy-number freely diffusing proteins during cell division. Some high-copy-number components are transiently associated almost immobile large assemblies (hyperstructures) and may be unequally distributed, contributing to phenotypic variability. We focus on...

Near-field scanning optical microscopy (NSOM) uses the near-field interaction of light from a sharp fiber-optic probe with sample interest to image surfaces at resolution beyond diffraction limit conventional optics. We used NSOM fluorescently labeled plasma membranes fixed human skin fibroblasts, either dried or in buffer. A patchy distribution fluorescent lipid analog suggestive domains was observed fixed, cells. The sizes these patches were consistent implied by fluorescence...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNested Polyhedra of MX2 (M = W, Mo; X S, Se) Probed by High-Resolution Electron Microscopy and Scanning Tunneling MicroscopyM. Hershfinkel, L. A. Gheber, V. Volterra, J. Hutchison, Margulis, R. TenneCite this: Am. Chem. Soc. 1994, 116, 5, 1914–1917Publication Date (Print):March 1, 1994Publication History Published online1 May 2002Published inissue 1 March...

In an effort to improve upon the recently reported cyclam based zinc sensor 1, "click"-generated 1,8-disubstituted analogue 2 has been prepared. The ligand shows a 2-fold increase in its fluorescence emission compared 1 exclusively presence of Zn(II) that is typical switch-on PET fluorescent sensors. Single crystal X-ray diffraction complexes model 10 reveals configuration adopted by macrocyclic framework extremely sensitive metal ion which it coordinates. For Zn(II), Mg(II), and Li(I) ions...

The original approach to calculating diffusion coefficients of a fluorescent probe from Fluorescence Recovery After Photobleaching (FRAP) measurements assumes bleaching with circular laser beam Gaussian intensity profile. This method was used without imaging the bleached cell. An empirical equation for rectangular geometry, created in confocal image, later published, however single allowing calculation arbitrary geometry does not exist. Our simulation allows computation regardless FRAP...

We demonstrate the direct printing of proteins on a surface using cantilevered nanopipette as probe scanned microscope. Protein features small ∼200 nm were directly delivered through ∼100 aperture by simply contacting with any surface. This allows for connection this methodology to standard separation techniques so that multiple can be printed one at different locations in ambient conditions.

The construction of amperometric immunosensors to cholera antitoxin immunoglobulins were shown have improved sensitivity when the toxin B subunit biorecognition entity was linked an electrogenerated biotinylated polypyrrole film copolymerized with pyrrole−lactobionamide monomer. copolymer exhibits greater permeability than polypyrrolic or polyphenolic films for permeation electroactive species. Hence, presence HRP marker immunoassay determined using hydroquinone, production quinone permeate...

The L-type calcium channel (LTCC) has a variety of physiological roles that are critical for the proper function many cell types and organs. Recently, member zinc-regulating family proteins, ZnT-1, was recognized as an endogenous inhibitor LTCC, but its mechanism action not been elucidated. In present study, using two-electrode voltage clamp recordings in Xenopus oocytes, we demonstrate ZnT-1-mediated inhibition LTCC critically depends on presence regulatory β-subunit. Moreover,...

Patches (lateral heterogeneities) of cell surface membrane proteins and lipids have been imaged by a number different microscopy techniques. This patchiness has taken as evidence for the organization membranes into domains whose composition differs from average entire membrane. However, mechanism specificity patch formation are not understood. Here we show how vesicle traffic to can create patches molecules size observed experimentally. Our computer model takes account lateral diffusion,...

Molecularly imprinted polymer (MIP) droplets were printed using a pipet or nano fountain pen on surface-enhanced Raman scattering (SERS)-active surfaces, to directly monitor the uptake and release of template molecule, β-blocking drug propranolol, by SERS. The monitored SERS bands can be related template, allowing for its detection but also identification in MIP. This is an advantage if technique used during development phase MIPs as microstructures, equally readout MIP-based biochips.

We describe the fabrication of polymer nanofibers with entrapped molecularly imprinted (MIP) nanoparticles and study their possible use in a fluorescence-based biosensor application. The MIP was fluorescent amino acid derivative dansyl-l-phenylalanine. Poly(vinyl alcohol) used as support for because it is water-soluble can be spun into very thin fibers. fibers were characterized by atomic force microscopy optical microscopy, fluorescence characterization target binding to MIP. show close...

Biomaterials folded into nanoparticles (NPs) can be utilized as targeted drug delivery systems for cancer therapy. NPs may provide a vehicle the anticancer lonidamine (LND), which inhibits glycolysis but was suspended from use at clinical trial stage because of its hepatotoxicity due to poor solubility and pharmacokinetic properties. The prepared by coassembly anionic polypeptide poly gamma glutamic acid (γ-PGA) designed amphiphilic positively charged peptide (designated mPoP-NPs) delivered...

Zinc transporter-1 (ZnT-1) is a putative zinc transporter that confers cellular resistance from toxicity. In addition, ZnT-1 has important regulatory functions, including inhibition of L-type calcium channels and activation Raf-1 kinase. Here we studied the effects on expression function T-type channels. Xenopus oocytes expressing voltage-gated channel (CaV) 3.1 or CaV3.2, enhanced low-threshold currents (I(caT)) to 182 ± 15 167.95 9.27% control, respectively (P < 0.005 for both channels)....

Abstract Microscope projection photolithography is combined with nanomolding and molecular imprinting for the fast microfabrication of molecularly imprinted polymer (MIP) arrays in form micrometric islands nanofilaments. Dot diameters from 70–90 μm are easily obtained using a 10× objective photomask carrying desired pattern. The dots composed parallel nanofilaments high aspect ratio, 150 nm diameter several micrometers length, which through procedure on porous alumina. small molecule...

Abstract One of the most promising strategies to treat cancer is use therapeutic antibodies that disrupt cell–cell adhesion mediated by dysregulated cadherins. The principal site where occurs encompasses Trp2 found at N‐terminal region protein. Herein, we employed naturally exposed highly conserved peptide Asp1‐Trp2‐Val3‐Ile4‐Pro5‐Pro6‐Ile7, as epitope prepare molecularly imprinted polymer nanoparticles (MIP‐NPs) recognize Since MIP‐NPs target responsible for adhesion, they were more potent...

Molecularly imprinted polymer nanogels (MIPs) as synthetic antibody mimics against the HAVDI epitope of N-cadherin specifically inhibit cell–cell aggregation and tumor cell invasiveness by blocking cadherin-mediated interactions.