A5030197147- Mesenchymal stem cell research
- Viral Infectious Diseases and Gene Expression in Insects
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Optimal Experimental Design Methods
- Cancer Immunotherapy and Biomarkers
- CRISPR and Genetic Engineering
- Protein purification and stability
- Immune cells in cancer
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- CAR-T cell therapy research
- Body Contouring and Surgery
Johns Hopkins University
2017-2022
Johns Hopkins Medicine
2020-2022
GlaxoSmithKline (United States)
2021
Creative Commons
2017
Biologic scaffolds synergize with immune checkpoint blockade to inhibit tumor growth in mice a process requiring T cells and macrophages.
Abstract Soft tissue reconstruction remains an intractable clinical challenge as current surgical options and synthetic implants may produce inadequate outcomes. deficits be surgically reconstructed using autologous adipose tissue, but these procedures can lead to donor site morbidity, require multiple procedures, have highly variable To address this need, we developed “off-the-shelf” extracellular matrix (ECM) biomaterial from allograft human (Acellular Adipose Tissue, AAT). We applied...
In cell culture process development, we rely largely on an iterative, one-factor-at-a-time procedure based experiments that explore a limited space. Design of (DoE) addresses this issue by allowing us to analyze the effects inputs responses systematically and efficiently. However, DoE cannot be applied directly study time-varying unless impractically large number bioreactors is used. Here, adopt methodology design dynamic (DoDE) incorporate feeding profiles efficiently in late-stage...
Chinese hamster ovary (CHO) cells are by far the most important mammalian cell lines used for producing antibodies and other therapeutic proteins. It is critical to fully understand their physiological conditions during a bioprocess in order achieve highest productivity desired product quality. Flow cytometry technology possesses unique advantages measuring multiple cellular attributes given examining changes culture heterogeneity over time that can be as metrics enhanced process...
ABSTRACT Soft tissue reconstruction remains an intractable clinical challenge as current surgical options and synthetic implants may produce inadequate outcomes. deficits be surgically reconstructed using autologous adipose tissue, but these procedures can lead to donor site morbidity, require multiple trips the operating room, have highly variable To address need for soft reconstruction, we developed “off-the-shelf” matrix from allograft human (acellular AAT). We applied physical chemical...
PURPOSE: Adipose tissue is used by surgeons for a variety of applications, including soft reconstruction and wound healing. However, harvesting adipose from patients presents challenges such as donor-site morbidity, outcome variability, difficulty in obtaining adequate volumes to correct large defects. To address this clinical challenge, we aimed create an “off-the-shelf” material using mechanical chemical processing techniques which remove lipids living cells while the into injectable form...