A5053170957- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- ATP Synthase and ATPases Research
- Advanced Proteomics Techniques and Applications
- Gene expression and cancer classification
- Protease and Inhibitor Mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Treatments and Mutations
- Cancer-related Molecular Pathways
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Redox biology and oxidative stress
- Nanoparticle-Based Drug Delivery
- Sulfur Compounds in Biology
- Lung Cancer Research Studies
- Lung Cancer Diagnosis and Treatment
- Immune Cell Function and Interaction
LungLife AI (United States)
2023-2024
GIRD Systems (United States)
2017-2019
Arizona State University
2009-2018
Translational Genomics Research Institute
2009-2011
Mayo Clinic in Arizona
2009-2011
Good Samaritan Medical Center
2009
University of Pennsylvania
2007
University of Cincinnati
2007
Purpose Sweat is a relatively unexplored biofluid for diagnosis and monitoring of disease states. In this study, the proteomic profiling immune‐related biomarkers from healthy individuals are presented. Experimental Design Eccrine sweat samples collected 50 individuals. LC‐MS/MS performed on two pools five male female participants. Individual analyzed by antibody isotyping microarrays ( n = 49), human cytokine arrays 30), quantitative ELISAs interleukin‐1α 16), epidermal growth factor 6),...
Blood circulates through nearly every organ including tumors. Therefore, plasma is a logical source to search for tumor-derived proteins and peptides. The challenge with that it complex bodily fluid composed of high concentrations normal host obscure identification molecules. To simplify plasma, we examined low molecular weight (LMW) fraction (plasma peptidome) using liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods. In the peptidome patients ductal adenocarcinoma pancreas...
An effective method of combating infectious diseases is the deployment hand-held devices at point-of-care (POC) for screening or self-monitoring applications. There a need very sensitive, low-cost and quantitative diagnostic devices. In this study, we present low-cost, multiplexed fluorescence detection platform that has high sensitivity wide dynamic range. Our system features inexpensive 3 × mm interference filters with stopband rejection, sharp transition edges, greater than 90%...
Quiescin sulfhydryl oxidase 1 (QSOX1) oxidizes groups to form disulfide bonds in proteins. We previously mapped a peptide plasma from pancreatic ductal adenocarcinoma (PDA) patients back an overexpressed QSOX1 parent protein. In addition overexpression cancer cell lines, 29 of 37 diagnosed with PDA expressed protein tumor cells, but was not detected normal adjacent tissues or transformed, nontumorigenic line. To begin evaluate the advantage might provide tumors, we suppressed expression...
Quiescin sulfhydryl oxidase 1 (QSOX1) oxidizes groups to form disulfide bonds in proteins. Tumor specific expression of QSOX1 has been reported for numerous tumor types. In this study, we investigate as a marker breast progression and evaluate the role it relates growth metastasis. Correlation with grade, subtype estrogen receptor (ER) status was gathered through informatic analysis using "Gene based Outcome Breast cancer Online" (GOBO) web-based tool. Expression protein tumors tissue...
// Paul D. Hanavan 1 , Chad R. Borges 2 Benjamin A. Katchman Douglas O. Faigel 3 Thai H. Ho Chen-Ting Ma 4 Eduard Sergienko Nathalie Meurice Joachim L. Petit F. Lake School of Life Sciences, Mayo Clinic Collaborative Research Building, Arizona State University, Scottsdale, AZ, USA Center for Personalized Diagnostics, Biodesign Institute, Tempe, Arizona, Conrad Prebys Chemical Genomics, Sanford-Burnham Medical La Jolla, CA, Correspondence to: Lake, e-mail: douglas.lake@asu.edu Keywords:...
This paper presents a new concept combining flexible organic light-emitting diode (OLED) display technology with fluorescent biorecognition microarray to fabricate point-of-care immunobiosensors. Our approach is designed leverage commercial OLED reduce pre-functionalized biosensor substrate costs pennies per cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> combined leveraging the industries ability manufacture an immense number of...
Abstract Point-of-care molecular diagnostics can provide efficient and cost-effective medical care they have the potential to fundamentally change our approach global health. However, most existing approaches are not scalable include multiple biomarkers. As a solution, we combined commercial flat panel OLED display technology with protein microarray enable high-density fluorescent, programmable, multiplexed biorecognition in compact disposable configuration clinical-level sensitivity. Our...
Mutations in TP53 induce autoantibody immune responses a subset of cancer patients, which have been proposed as biomarkers for early detection. Here, we investigate the association p53-specific autoantibodies with multiple tumor subtypes and determine p53 mutation status epitope specificity.
Over 600 000 cancers each year are attributed to the human papillomavirus (HPV), including cervical, anogenital and oropharyngeal (OPC). A key challenge in understanding HPV immunobiology is diversity of oncogenic types need for multiplexed display antigens measure antibody responses. We have generated custom protein microarrays displaying 98 proteins as C‐terminal GST fusion proteins, representing eight two low‐risk (HPV6 11) ten high‐risk (HPV16, 18, 31, 33, 35, 39, 45, 51, 52 58)....
Abstract Purpose Computed tomography is the standard method by which pulmonary nodules are detected. Greater than 40% of biopsies not lung cancer and therefore necessary, suggesting that improved diagnostic tools needed. The LungLB™ blood test was developed to aid clinical assessment indeterminate suspicious for cancer. identifies circulating genetically abnormal cells (CGACs) present early in pathogenesis. Methods a 4-color fluorescence in-situ hybridization assay detecting CGACs from...
This work explored the viability of a new miniaturized fluorescence measurement-based sensor architecture using organic light emitting diode (OLED) display and photodiode active matrix array technology for point-of-use diagnosis multiple disease biomarkers in low cost disposable configuration. Sensor feasibility optical performance were evaluated bright 0.3mW/mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> 2 × mm, 455nm blue OLED...
This work details the design and characterization of a low-cost, portable highly sensitive fluorescence detection system intended for use in compact disposable point-of-care (POC) device. The device leverages time integration to improve signal noise ratio (SNR) compared instantaneous measurements. It also eliminates complicated focusing optics electronics typically found bulky expensive laboratory-scale devices. Characterization was performed by measuring series logarithmically scaled...
The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one the key roadblocks limiting transition this technology from clinical laboratory to point-of-care in low-to-middle income countries (LMICs). As a solution, we present low-cost system architecture for fluorescence-based using small volume sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low consumables conventional glass slides, pre-printed biorecognition molecules. We...
The PathogenDx EnviroX-F uses microarray technology to simultaneously detect Listeria species, monocytogenes, and Salmonella species from environmental samples without the need for enrichment.The validation study included a matrix of four matrices (stainless steel, plastic, rubber, sealed concrete) comparing assay US Food Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Chapter 10, "Detection L. monocytogenes in Foods Environmental Samples, Enumeration Foods" (revised March...
Abstract Quiescin-sulfhydryl oxidase (QSOX1) oxidizes sulfhydryl groups to form disulfide bonds in proteins. We previously mapped a peptide plasma from pancreatic ductal adenocarcinoma patients back an overexpressed QSOX1 parent protein tumor cells. It is not known why, or what capacity, tumors overexpress QSOX1. To begin address this question, we first showed through immunohistochemistry that diagnosed with cancer, while it expressed adjacent normal tissue infiltrating lymphocytes....
Abstract Background The PathogenDx EnviroX-Rv uses endpoint PCR + DNA microarray technology to detect SARS-CoV-2, the causative agent of COVID-19, from stainless-steel environmental sample swabs. Objective To validate assay as part Emergency Response Validation (ERV) Performance Tested Method(s)SM (PTM) program. Method was evaluated for specificity using in silico analysis ≥41 000 SARS-CoV-2 sequences and over 50 exclusivity organisms (both near neighbors background organisms). candidate...